Prevalence of <i>Giardia duodenalis</i> Among Dogs in China from 2001 to 2021: A Systematic Review and Meta-Analysis

Zhao, Ziyu; Li, Ming-Han; Lyu, Chuang; Meng, Xiang-Zhu; Qin, Yifeng; Yang, Xin-Bo; Ma, Ning; Zhao, Quan; Zhang, Yuan; Jiang, Jing

doi:10.1089/fpd.2021.0073

Cited by 7 publications

(10 citation statements)

References 47 publications

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“…However, other genotypes/assemblages show host-speci city. For example, assemblage E mostly infects cattle, while assemblages C and D infect dogs, and assemblage F infects cats [12,32]. However, our study did not report any positive cases of Giardia in cats or assemblages D and C in dogs.…”

Section: Full Textcontrasting

confidence: 76%

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Assessing the Public Health and Zoonotic Impacts of Giardia duodenalis Assemblages in Domestic Animals of Southwestern Iran

Asghari,

Motazedian,

Asgari

et al. 2024

Preprint

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Domestic animals play a vital role in the spread and transmission of various zoonotic agents to humans. Giardia duodenalis, an important enteric zoonotic parasite, is widespread globally. This research aimed to assess the presence and genetic diversity of G. duodenalis assemblages in domestic animals in Shiraz, southern Iran. 245 fresh fecal samples were collected from 87 dogs, 63 cats, and 95 cattle in Shiraz, the capital city of Fars province, between July 2021 and August 2022. None of the animals showed gastrointestinal signs such as diarrhea, and all fecal samples had normal consistency. Upon parasite observation using light microscopy, a DNA fragment of G. duodenalis tpi gene was amplified using nested-PCR. Using direct wet mount and Wheatley’s trichrome staining examination, 9 out of 245 samples (3.7%; 1 from dogs and 8 from cattle) tested positive for G. duodenalis. Molecular methods confirmed 1.1% (1/87) of dogs and 8.4% (8/95) of cattle to be positive. Giardiasis was not detected in cats. Phylogenetic analysis revealed that Giardia isolates infecting dogs and cattle in Shiraz belonged to three genotypes/assemblages: A, B (zoonotic), and E (non-zoonotic). In cattle, assemblages E (75%; 6/8), A (12.5%; 1/8), and B (12.5%; 1/8) were identified, while dogs were infected with assemblage A (100%; 1/1). In Shiraz, southwestern Iran, domestic animals like cattle and dogs could potentially serve as reservoirs for zoonotic infections caused by G. duodenalis.

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Section: Full Textcontrasting

confidence: 76%

“…Domestic animals play a crucial role in the food chain for the human population. Therefore, they are raised and kept close to humans, especially in rural areas [9,12,13]. The proximity between animals and humans can potentially lead to an increased risk of transmitting zoonotic agents like G. duodenalis.…”

Section: Full Textmentioning

confidence: 99%

Assessing the Public Health and Zoonotic Impacts of Giardia duodenalis Assemblages in Domestic Animals of Southwestern Iran

Asghari,

Motazedian,

Asgari

et al. 2024

Preprint

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“…G. duodenalis is widely detected in humans, non-human primates (NHPs), ruminants, companion animals, livestock, and wild animals and even in the environment in China [ 17 ]. The estimated prevalence of G. duodenalis among dogs in China was 9.3% and 14.3% by microscopy and serology, respectively [ 18 ]. Molecular analysis revealed that G. duodenalis was detected in approximately 12.3% of dogs in China, including aggregates A, B, C, D, E and F [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…The estimated prevalence of G. duodenalis among dogs in China was 9.3% and 14.3% by microscopy and serology, respectively [ 18 ]. Molecular analysis revealed that G. duodenalis was detected in approximately 12.3% of dogs in China, including aggregates A, B, C, D, E and F [ 18 ]. However, limited information about pet dogs infected with G. duodenalis is available in central China [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and genetic diversity of Giardia duodenalis in pet dogs from Zhengzhou, central China and the association between gut microbiota and fecal characteristics during infection

et al. 2022

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“…Several host (such as age and health status) and parasite factors (such as the virulence of the parasite and the number of cysts in hosts) may substantially impact the evolution and severity of G. duodenalis infection [10]. Consumption of contaminated food / water sources and direct contact with infected animals are the primary transmission routes for G. duodenalis infection [11].…”

Section: Introductionmentioning

confidence: 99%

Clinical Manifestations and Molecular Identification of Giardia duodenalis in Pediatric Cancer Patients in Shiraz, Southwestern Iran

Mahdavi,

Mohammadi,

Shamsi

et al. 2024

Preprint

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Purpose Understanding the prevalence of intestinal parasitic infections in the general population is crucial, especially in individuals with cancer. This study aimed to investigate the clinical and molecular characteristics of Giardia duodenalis (G. duodenalis) infection and identify potential risk factors in children and teenagers with malignancies in Shiraz, southwestern Iran. Methods 200 fresh fecal samples were collected from children and adolescents suffering from 32 different cancer types at Amir, Nemazee, and Saadi hospitals affiliated with Shiraz University of Medical Sciences between October 2021 to May 2023. Direct microscopy using saline and iodine wet mount was conducted, and all fecal samples were rechecked by SSU-PCR. Subsequently, a DNA fragment from the tpi gene was amplified on SSU-PCR positive samples for sequencing and assemblage identification. Results Our study found a 4% (8/200) prevalence of G. duodenalis using microscopy and PCR. The molecular findings were consistent with the microscopic results. All eight positive samples with SSU-rRNA gene were also detected as positive with tpi gene and were correctly sequenced. Among the examined cancer patients, two assemblages were identified: A [sub-assemblage AI (2/8, 25%) and sub-assemblage AII (3/8, 37.5%)] and B [sub-assemblage BIV (3/8, 37.5%)]. Notably, patients were more vulnerable to G. duodenalis infection after receiving at least 8 treatment episodes (P < 0.05) and displaying gastrointestinal symptoms (P > 0.05). The demographic characteristics of cancer patients with Giardia infection and the statistical conclusions were separately detailed. Conclusions The small sample size and low prevalence rate in this study hindered precise epidemiological conclusions. Nonetheless, the results suggest that G. duodenalis infection among cancer patients in Shiraz city originates from humans, without any specific animal groups (C-H) involved. Additional molecular-based analyses are required to verify and build upon these findings.

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Prevalence of Giardia duodenalis Among Dogs in China from 2001 to 2021: A Systematic Review and Meta-Analysis

Cited by 7 publications

References 47 publications

Assessing the Public Health and Zoonotic Impacts of Giardia duodenalis Assemblages in Domestic Animals of Southwestern Iran

Assessing the Public Health and Zoonotic Impacts of Giardia duodenalis Assemblages in Domestic Animals of Southwestern Iran

Prevalence and genetic diversity of Giardia duodenalis in pet dogs from Zhengzhou, central China and the association between gut microbiota and fecal characteristics during infection

Clinical Manifestations and Molecular Identification of Giardia duodenalis in Pediatric Cancer Patients in Shiraz, Southwestern Iran

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