Prevalence of<i>Entamoeba histolytica</i>and other intestinal parasites in a community from Maracaibo, Venezuela

Chacín-Bonilla, Leonor; Bonilla, Ernesto; Parra, Alexander; Estévez, Jesús; Morales, Liliana; Suárez, Héctor

doi:10.1080/00034983.1992.11812680

Cited by 12 publications

(9 citation statements)

References 14 publications

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“…histolytica considerably contributes to diarrheal burden and childhood mortality [62]. Whereas some studies report little variation in prevalence of Entamoeba with age [63], others find the contrary [59, 64, 65] and thus the results of these studies may not be directly comparable with ours. However, with few exceptions [52, 54], the reported prevalence is usually much lower (< 25%, [59, 61, 63–66]) than what we have found.…”

Section: Discussioncontrasting

confidence: 64%

“…However, with few exceptions [52, 54], the reported prevalence is usually much lower (< 25%, [59, 61, 63–66]) than what we have found. Although the discrepancy could be explained by lower sensitivity of microscopy in some cases [63, 65], Ouattara et al [54] found E . coli in 62% of examined children in Cote d'Ivoire using this method, possibly reflecting high parasite burden.…”

Section: Discussionmentioning

confidence: 99%

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Use of shotgun metagenomics for the identification of protozoa in the gut microbiota of healthy individuals from worldwide populations with various industrialization levels

et al. 2019

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Protozoa have long been considered undesirable residents of the human gut, but recent findings suggest that some of them may positively affect the gut ecosystem. To better understand the role and ecological dynamics of these commensal and potentially beneficial protozoan symbionts, we need efficient methods to detect them, as well as accurate estimates of their prevalence across human populations. Metagenomics provides such an opportunity, allowing simultaneous detection of multiple symbionts in a single analytical procedure. In this study, we collected fecal samples of 68 individuals from three Cameroonian populations with different subsistence modes and compared metagenomics-based and targeted methods of detection for two common protozoan genera: Blastocystis and Entamoeba. In addition, we analyzed our data along with publicly available fecal metagenomes from various worldwide populations to explore the prevalence and association patterns of ten protozoan genera. Regarding the detection method, microscopy was much less sensitive than metagenomics for Entamoeba, whereas qPCR was at least as sensitive as metagenomics for Blastocystis sp. However, metagenomics was more likely to detect co-colonizations by multiple subtypes. Out of the ten examined genera in 127 individuals from Cameroon, Tanzania, Peru, Italy or USA, only three (Blastocystis, Entamoeba and Enteromonas) had an overall prevalence exceeding 10%. All three genera were more common in less industrialized populations and their prevalence differed between continents and subsistence modes, albeit not in a straightforward manner. The majority (72.5%) of colonized individuals carried at least two protozoan species, indicating that mixed-species colonizations are common. In addition, we detected only positive and no negative association patterns between different protozoa. Despite the pitfalls of the metagenomic approach, ranging from the availability of good-quality sequencing data to the lack of standard analytical procedures, we demonstrated its utility in simultaneous detection of multiple protozoan genera, and especially its ability to efficiently detect mixed-species colonizations. Our study corroborates and expands prevalence results previously obtained for Blastocystis sp. and provides novel data for Entamoeba spp. and several other protozoan genera. Furthermore, it indicates that multiple protozoa are common residents of the healthy human gut worldwide.

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Section: Discussioncontrasting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Use of shotgun metagenomics for the identification of protozoa in the gut microbiota of healthy individuals from worldwide populations with various industrialization levels

et al. 2019

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“…En el caso de T. trichiura, que fue uno de los geohelmintos de mayor prevalencia (74,1%), los resultados coinciden con los de otros autores que lo señalan como el enteroparásito más común en Venezuela (11,28,34,(38)(39)(40) y el Estado Bolívar (31, 41) principalmente en comunidades rurales.…”

Section: Discussionunclassified

Parasitosis intestinales en escolares: relación entre su prevalencia en heces y en el lecho subungueal.

Rumhein¹,

Sánchez²,

Requena³

et al. 2005

Rev Biomed

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Introducción. Los parásitos intestinales de forma general son transmitidos a través de la ingestión de agua y/o alimentos contaminados con formas infectantes. También se ha llamado la atención acerca del papel del lecho subungueal como posible diseminador de enteropatógenos en personas con hábitos de higiene inadecuados. Objetivo. Determinar la prevalencia de parásitos intestinales en las heces y en el lecho subungueal en escolares de la U.E.E. Teresa de la Parra del Barrio Buen Retiro en San Félix, Estado Bolívar, Venezuela. Material y Métodos. De mayo a julio de 2003 fueron evaluados 344 niños, entre 6 y 15 años. De cada uno fue obtenida una muestra de heces y analizada mediante la técnica de examen directo y los métodos de concentración de Willis y formol-éter. También fueron colectadas muestras de uñas y material subungueal siendo analizadas mediante la técnica de formol-éter. Resultados. La prevalencia de parásitos en heces fue de 97.4% (335/344). No hubo predilección en cuanto a la edad (χ2 = 6.2; g.l. = 4) o sexo de los parasitados (p › 0,05). Los protozoarios fueron más prevalentes, destacando Blastocystis hominis con 76.2%. Entre los helmintos el más común fue Trichuris trichiura con 74.1%. La prevalencia de estadios parasitarios en el deposito subungueal fue de 3.6% (11/307), siendo A. lumbricoides y E. coli los más comunes. No hubo relación entre la presencia de parásitos en el lecho subungueal y en las heces. Conclusiones. Se determinó una elevada prevalencia de parasitosis intestinales en los escolares evaluados. Aunque se demostró la importancia del lecho subungueal como diseminador de enteroparásitos, este mecanismo no parece ser el principal implicado en la cadena epidemiológica de estas parasitosis en los niños estudiados.

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“…Human cases of infection with the uninucleated cyst-producing Entamoeba species referred to as Entamoeba polecki are considered to be rare (2,4), except in Papua New Guinea, where prevalence rates as high as 30% are reported (1,5), and are often associated with contact with pigs. However, eight cases of human infection with a uninucleated cyst-producing Entamoeba species have been reported; these cases resulted from contact with monkeys, and the agent was identified as E. chattoni (6).…”

mentioning

confidence: 99%

Genetic Variation among Human Isolates of Uninucleated Cyst-Producing Entamoeba Species

2001

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Twelve human infections with Entamoeba spp. producing uninucleated cysts were studied. DNA was extracted from infected feces and used to amplify part of the ameba small-subunit rRNA gene. Sequence analysis identified four distinct types of Entamoeba, all of which are related to Entamoeba polecki and E. chattoni and two of which have not been reported previously. Whether these genetic types represent different species is unclear. We propose that the agent of all human infections with uninucleated cyst-producing Entamoeba species be reported as "E. polecki-like."

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Prevalence ofEntamoeba histolyticaand other intestinal parasites in a community from Maracaibo, Venezuela

Cited by 12 publications

References 14 publications

Use of shotgun metagenomics for the identification of protozoa in the gut microbiota of healthy individuals from worldwide populations with various industrialization levels

Use of shotgun metagenomics for the identification of protozoa in the gut microbiota of healthy individuals from worldwide populations with various industrialization levels

Parasitosis intestinales en escolares: relación entre su prevalencia en heces y en el lecho subungueal.

Genetic Variation among Human Isolates of Uninucleated Cyst-Producing Entamoeba Species

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