Prevalence of <i>Cyclospora cayetanensis</i> among symptomatic and asymptomatic immune-competent children less than five years of age in Alexandria, Egypt

Massoud, Naguib M.; Said, Doaa E; El-Salamouny, Ahmed R.

doi:10.1016/j.ajme.2012.02.008

Cited by 8 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several species of Microsporidia have emerged as opportunistic agents in immunocompromised patients and have also been reported in immunocompetent patients (Tremoulet et al, 2004). The detected prevalence rate of Microsporidia in this study was nearly equal to previous studies in Assuit (4.2%) (Zaghlool, 2003) and Egypt (4.8%, 5%) (El-Mahallawy et al, 2011;Massoud et al, 2012). During the present study, E. coli, Cryptosporidium spp.…”

Section: G Lamblia Followed Bysupporting

confidence: 74%

Prevalence of Intestinal Parasites among Children Attending Assiut University Children’s Hospital, Assiut, Egypt

Monib¹,

Hassan²,

Attia³

et al. 2016

J. Adv. Parasitol.

View full text Add to dashboard Cite

Section: G Lamblia Followed Bysupporting

confidence: 74%

Prevalence of Intestinal Parasites among Children Attending Assiut University Children’s Hospital, Assiut, Egypt

Monib¹,

Hassan²,

Attia³

et al. 2016

J. Adv. Parasitol.

View full text Add to dashboard Cite

“…In contrast, all of the cases having profile IV were GIT asymptomatic suggesting that this genotype is less virulent than are the others. In agreement with these results, prevalence of Cyclospora infection in asymptomatic children was 6 % compared to 17 % in symptom atic ones [Massoud et al 2012]. In conclusion, PCR/HRM was proved a wide variation on C. cayetanensis genotypes that could be related to its virulence degree.…”

Section: Discussionsupporting

confidence: 71%

“…The infection rate ranged from 11-29.8 % among patients with gastroenteritis (Alakpa and Fagbenro-Beyioku 2002). In Egypt, there was a significant difference between prevalence of Cyclosporainfected children in symptomatic (17 %) and asymptomatic (6 %) groups in Alexandria (Massoud et al 2012). Other study in Ismailia detected that Cyclospora oocysts were 19.6 % of 230 diarrheal children with immunocompetent conditions and 34.6 % of 230 diarrheal children with immunocompromised conditions (Abdel-Wahab et al 2008).…”

Section: Introductionmentioning

confidence: 98%

Wide genetic variations at 18S ribosomal RNA locus of Cyclospora cayetanensis isolated from Egyptian patients using high resolution melting curve

et al. 2016

View full text Add to dashboard Cite

A variable clinical picture of cyclosporiasis including gastrointestinal tract (GIT) symptomatic or asymptomatic beside extraintestinal consequences suggests a possibility of heterogenicity of Cyclospora cayetanensis. The present work aimed to explore the possibility of genetic variation of C. cayetanensis using high-resolution melting (HRM) curve of polymerase chain reaction (PCR) amplified 18S rRNA genes. DNAs extracted from the stool samples of 70 cyclosporiasis patients were amplified and scanned by PCR/HRM curve. The results showed that there are four different genotypic profiles of C. cayetanensis with presence of mixed ones. Although Tm of all profiles was within the same range, they were discerned by plotting of the temperature-shifted florescence difference between normalized melting curves (dF/dT). Genotypic profile I was found alone in 40 % of patients and mixed with genotypic profile II and/or III in 25.7 % of patients, followed by genotypic profile II in 14.3 % then genotypic profile III and IV (10 % each). A significant relation was found between genotypic profiles and GIT symptomatic status as profile I and profile II were mostly detected in patients with acute GIT symptoms without or with chronic illness, respectively, while profile IV cases only were GIT asymptomatic. Statistical significance relations between genotypic profiles and age, gender, residence and oocyst shape index were determined. In conclusion, PCR/HRM proved a wide variation on C. cayetanensis genes that could be reflected on its pathogenic effects and explaining the variability of the clinical manifestations presented by cyclosporiasis patients.

show abstract

“…Naguib et al included the children less than 5 years of age group and they observed that infection is most commonly seen in the age group less than 2 years as compared to more than 2 years. 13 Our study, for example, have reported parasitic infestation to be greater in 5 to 12 year age group than the below 5 year group. This could be due to the reason that children in this age are most active; they remain outdoors constantly playing in the soil with poor hygiene conditions and eat with unwashed hands.…”

Section: Discussionmentioning

confidence: 45%

An analytical study of intestinal parasitosis in children

Bhattacharya

Barik

Bhattacharya

et al. 2017

Int J Community Med Public Health

View full text Add to dashboard Cite

Background: Intestinal parasitosis (IP) is an important public health problem worldwide, most commonly seen in school age children and lead to nutritional deficiency, anemia and impaired cognition. Dearth of data regarding parasitic prevalence in the pediatric population triggered such a retrospective study planned by the Departments of Pediatrics and Microbiology, of two Medical Colleges whereby all the symptomatic children (>1 year and <12 years, divided into less than 5 year and 5-12 year groups) were examined for IP.Methods: Total 1000 parasite positive stool samples were collected for a period of two years and included for the study. Routine macroscopic and microscopic (saline and Lugol’s iodine wet mounts) examinations were carried out for the presence of ova, cysts and parasites.Results: Out of the 1000 positive samples, Enterobius vermicularis was predominant with 287 (28.7%) of all cases. The next in order were Giardia lamblia 243 (24.3%), Entamoeba histolytica 219 (21.9%), Ascaris lumbricoides 143 (14.3%), Ancylostoma duodenale 39 (3.9%), Taenia 27 (2.7%) and others 42 (4.2%). The distribution of parasites were heavier among the 5 to 12 year group (74.6%) than the below 5 year one (25.4%), with single parasitic isolate. Lack of hygienic practices like open field defecation, faecal contamination of water and improper hand washing aggravate infestation.Conclusions: Thus our study revealed that steps are to be taken by both the government and society to promote healthcare awareness in mothers, and mass scale deworming through school campaigns in order to lower the parasitic burden in children.

show abstract

Prevalence of Cyclospora cayetanensis among symptomatic and asymptomatic immune-competent children less than five years of age in Alexandria, Egypt

Cited by 8 publications

References 33 publications

Prevalence of Intestinal Parasites among Children Attending Assiut University Children’s Hospital, Assiut, Egypt

Prevalence of Intestinal Parasites among Children Attending Assiut University Children’s Hospital, Assiut, Egypt

Wide genetic variations at 18S ribosomal RNA locus of Cyclospora cayetanensis isolated from Egyptian patients using high resolution melting curve

An analytical study of intestinal parasitosis in children

Contact Info

Product

Resources

About