Prevalence of <i>Borrelia Burgdorferi</i> Sensu Lato Genomospecies and of the Human Granulocytic Ehrlichiosis (HGE) Agent in <i>Ixodes Ricinus</i> Ticks Collected in the Area of Monti Lepini, Italy

Santino, Iolanda; Iori, A; Nicoletti, Mauro; Valletta, S; Cimmino, Carmen; Scoarughi, Gian Luca; Santapaola, Daniela; Sessa, Rosa; M, Del Piano

doi:10.1177/039463200301600203

Cited by 13 publications

(8 citation statements)

References 10 publications

(12 reference statements)

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“…burgdorferi s.l. is currently considered the most common tick-borne pathogen in the northern hemisphere and it has been frequently detected in ticks and wild animals in Italy [17][18][19][20][21][22]. Several vertebrates are involved in the cycle of this agent, but the role of wild boars is currently not defined [7].…”

Section: Resultsmentioning

confidence: 99%

Zoonotic tick-borne bacteria among wild boars (Sus scrofa) in Central Italy

Ebani¹,

Ebani²,

Bertelloni³

et al. 2017

APJTD

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Section: Resultsmentioning

confidence: 99%

Zoonotic tick-borne bacteria among wild boars (Sus scrofa) in Central Italy

Ebani¹,

Ebani²,

Bertelloni³

et al. 2017

APJTD

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“…Many investigations in Italy have been conducted in mountain and wild habitats (Cacciapuoti et al., 1995; Cinco et al., 1997, 1998; Ciceroni et al., 1998; Mannelli et al., 1999, 2003; Favia et al., 2001; Rizzoli et al., 2002; Santino et al., 2002, 2003; Bertolotti et al., 2006; Mantelli et al., 2006; Piccolin et al., 2006; Pecchioli et al., 2007). However, there is a lack of studies performed in periurban parks.…”

Section: Discussionmentioning

confidence: 99%

Study on Ticks and Tick‐Borne Zoonoses in Public Parks in Italy

Corrain

Drigo

Fenati

et al. 2012

Zoonoses and Public Health

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A survey on tick density and on tick-borne zoonoses was carried out in four public parks in the outskirts of Imola (northern Italy) from June to October 2006. All stages of Ixodes ricinus and only larvae of Riphicephalus sanguineus were recovered by dragging, performed on 100-m transects. Almost all ticks (99%) were harvested in one park. I. ricinus density (nymphs/100 m(2) ) ranged from 0 in park L to 6.3 in park F. Nymphs and adults of I. ricinus were subjected to PCR for Anaplasma phagocytophilum, Bartonella spp., Borrelia burgdorferi s. l. and Rickettsia spp. The observed prevalences were 38.3% for Bartonella henselae, 5.2% for Bartonella clarridgeiae, 10.4% for B. burgdorferi s. l., 2.6% for Rickettsia helvetica and 13% for Rickettsia monacensis, respectively. No DNA of A. phagocytophilum was found. Acarological risks (AR) were calculated as probabilities of collecting at least one infected nymph per transect. The AR values calculated for the various zoonotic agents were 11.4% for R. helvetica, 27.7% for B. clarridgeiae, 49.7% for B. burgdorferi s. l., 57.2% for R. monacensis and 90.4% for B. henselae, respectively. In this study, B. clarridgeiae was for the first time identified in I. ricinus ticks.

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“…Mixed infections with two or more Borrelia species are frequent in ticks (Santino et al , 1998; Santino et al , 2003) and have been reported also in human patients (Ruzic‐Sablijic et al , 2005). The present study indicates that human patients with Lyme borreliosis can simultaneously harbor different B. burgdorferi s.l .…”

Section: Discussionmentioning

confidence: 99%

Detection of Borrelia burgdorferi sensu lato DNA by PCR in serum of patients with clinical symptoms of Lyme borreliosis

Santino

Berlutti

Pantanella

et al. 2008

FEMS Microbiology Letters

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Lyme borreliosis is a disease caused by spirochaetes belonging to the genospecies complex Borrelia burgdorferi sensu lato (s.l.) transmitted by Ixodes ticks. At present, serology remains the main diagnostic tool for laboratory diagnosis of Lyme borreliosis. Recently, the PCR technique has been applied for diagnosis of B. burgdorferi s.l., but, until now, a reliable, easy-to-perform and sensitive method has not been described. Here we present a new PCR-based method for the detection of both B. burgdorferi s.l. and Borrelia genospecies DNAs in serum samples collected from patients showing Lyme disease symptoms. Of 265 serum samples of patients included in this study, 7.5% were positive, 1.9% was borderline and 90.6% were negative for antibodies against B. burgdorferi by enzyme-linked immunosorbent assay and Western blotting. The B. burgdorferi s.l. 16S rRNA gene was detected by PCR in all serum-positive and in two borderline samples. None of the serum-negative samples nor serum samples collected from healthy subjects gave positive PCR reactions. Of PCR-positive serum samples, 50% gave a positive reaction for Borrelia afzelii, 18% for Borrelia garinii and 23% for two Borrelia species. Two samples (9%) were not identified to species level. The new protocol could be considered to be reliable as neither false-positive nor false-negative reactions were recorded, and to be sensitive as it detects DNA from one bacterial cell.

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Prevalence of Borrelia Burgdorferi Sensu Lato Genomospecies and of the Human Granulocytic Ehrlichiosis (HGE) Agent in Ixodes Ricinus Ticks Collected in the Area of Monti Lepini, Italy

Cited by 13 publications

References 10 publications

Zoonotic tick-borne bacteria among wild boars (Sus scrofa) in Central Italy

Zoonotic tick-borne bacteria among wild boars (Sus scrofa) in Central Italy

Study on Ticks and Tick‐Borne Zoonoses in Public Parks in Italy

Detection of Borrelia burgdorferi sensu lato DNA by PCR in serum of patients with clinical symptoms of Lyme borreliosis

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