Prevalence of <i>Anaplasmataceae</i> and <i>Filariidae</i> species in unowned and military dogs in New Caledonia

Dahmani, Mustapha; Tahir, Djamel; Cabre, Olivier; Raoult, Didier; Fénollar, Florence; Davoust, Bernard; Mediannikov, Oleg

doi:10.1002/vms3.97

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…Five different haplotypes of D. immitis were detected, with the haplotype 1 being the most common worldwide, and haplotype 2 described causing human ocular dirofilariasis in South America 32 , 33 . The three other haplotypes have been described by only one DNA sequence each, obtained during routine epidemiological monitoring 34 – 36 . The presence of genetic diversity within D. immitis was confirmed by previous results based on the complete NADH dehydrogenase 1 (ND1) and 16S rRNA gene sequences of isolates from China, which highlighted two distinct phylotypes 37 .…”

Section: Discussionmentioning

confidence: 99%

Genetic and geographical delineation of zoonotic vector-borne helminths of canids

Laidoudi

Bedjaoui

Latrofa

et al. 2022

Sci Rep

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Several zoonotic vector-borne helminths (VBHs) infesting canids cause serious veterinary and medical diseases worldwide. Increasing the knowledge about their genetic structures is pivotal to identify them and therefore to settle effective surveillance and control measures. To overcome the limitation due to the heterogeneity of large DNA sequence-datasets used for their genetic characterization, available cytochrome c oxidase subunit 1 (cox1) (n = 546) and the 12S rRNA (n = 280) sequences were examined using combined bioinformatic approach (i.e., distance-clustering, maximum likelihood phylogeny and phylogenetic evolutionary placement). Out of the 826 DNA available sequences from GenBank, 94.7% were characterized at the haplotype level regardless sequence size, completeness and/or their position. A total of 89 different haplotypes were delineated either by cox1 (n = 35), 12S rRNA (n = 21) or by both genes (n = 33), for 14 VBHs (e.g., Acanthocheilonema reconditum, Brugia spp., Dirofilaria immitis, Dirofilaria repens, Onchocerca lupi and Thelazia spp.). Overall, the present approach could be useful for studying global genetic diversity and phylogeography of VBHs. However, as barcoding sequences were restricted to two mitochondrial loci (cox1 and 12S rRNA), the haplotype delineation proposed herein should be confirmed by the characterization of other nuclear loci also to overcome potential limitations caused by the heteroplasmy phenomenon within the mitogenome of VBHs.

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Section: Discussionmentioning

confidence: 99%

Genetic and geographical delineation of zoonotic vector-borne helminths of canids

Laidoudi

Bedjaoui

Latrofa

et al. 2022

Sci Rep

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The global status of Dirofilaria immitis in dogs: a systematic review and meta-analysis based on published articles

Anvari

Narouei

Daryani

et al. 2020

Research in Veterinary Science

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Assessment of a metabarcoding approach for the characterisation of vector-borne bacteria in canines from Bangkok, Thailand

et al. 2019

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Background Globally, bacterial vector-borne disease (VBD) exerts a large toll on dogs in terms of morbidity and mortality but nowhere is this more pronounced than in the tropics. Tropical environments permit a burgeoning diversity and abundance of ectoparasites some of which can transmit an extensive range of infectious agents, including bacteria, amongst others. Although some of these vector-borne bacteria are responsible for both animal and human diseases in the tropics, there is a scarcity of epidemiological investigation into these pathogens’ prevalence. The situation is further exacerbated by frequent canine co-infection, complicating symptomatology that regular diagnostic techniques may miss or be unable to fully characterise. Such limitations draw attention to the need to develop screening tools capable of detecting a wide range of pathogens from a host simultaneously. Results Here, we detail the employment of a next-generation sequencing (NGS) metabarcoding methodology to screen for the spectrum of bacterial VBD that are infecting semi-domesticated dogs across temple communities in Bangkok, Thailand. Our NGS detection protocol was able to find high levels of Ehrlichia canis , Mycoplasma haemocanis and Anaplasma platys infection rates as well as less common pathogens, such as “ Candidatus Mycoplasma haematoparvum”, Mycoplasma turicensis and Bartonella spp. We also compared our high-throughput approach to conventional endpoint PCR methods, demonstrating an improved detection ability for some bacterial infections, such as A. platys but a reduced ability to detect Rickettsia . Conclusions Our methodology demonstrated great strength at detecting coinfections of vector-borne bacteria and rare pathogens that are seldom screened for in canines in the tropics, highlighting its advantages over traditional diagnostics to better characterise bacterial pathogens in environments where there is a dearth of research. Electronic supplementary material The online version of this article (10.1186/s13071-019-3651-0) contains supplementary material, which is available to authorized users.

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Prevalence of Anaplasmataceae and Filariidae species in unowned and military dogs in New Caledonia

Cited by 3 publications

References 31 publications

Genetic and geographical delineation of zoonotic vector-borne helminths of canids

Genetic and geographical delineation of zoonotic vector-borne helminths of canids

The global status of Dirofilaria immitis in dogs: a systematic review and meta-analysis based on published articles

Assessment of a metabarcoding approach for the characterisation of vector-borne bacteria in canines from Bangkok, Thailand

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