Prevalence of Human Papillomavirus Infection in a Kashmiri Ethnic Female Population

Asiaf, Asia; Ahmad, S.; Zargar, Mohammad Afzal; Mufti, Shahida Masarat; Mir, Syed

doi:10.1089/gtmb.2011.0354

Cited by 10 publications

(5 citation statements)

References 29 publications

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“…PCR. Both the samples were screened for the presence of HPV infection by PCR to amplify a 450-bp fragment from the conserved region in all HPV variants with MY09/MY11 primers: forward, 5'-CGTCCMARRGGAWACTGATC-3' and reverse, 5'-GCMCAGGGWCATAAYAATGG-3' as the reverse primer (34). PCR was performed using 200 ng of DNA along with dNTPs (0.2 mM each), Taq polymerase (cat.…”

Section: Characterizationmentioning

confidence: 99%

Establishment and characterization of novel autologous pair cell lines from two Indian non‑habitual tongue carcinoma patients

et al. 2022

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Oral tongue squamous cell carcinoma (OTSCC) is one of the major causes of fatality in India due to very high percentage of patients with habits of smoking and chewing tobacco and associated products. Being highly heterogeneous in nature, every patient poses a different challenge clinically. To understand disease progression in an improved way, knowledge of cross-talk between tumor stroma and the tumor cells becomes indispensable. Patient-derived in vitro cell line models are helpful to understand the complexity of diseases. However, they have very low efficiency of establishment from the tumor samples, particularly the cancer-associated fibroblasts (CAFs). In the present study, two novel autologous pairs were immortalized spontaneously from non-habitual, HPV-positive patients, who presented with OTSCC. The epithelial and fibroblast cell lines had typical polygonal and spindle-shaped morphology, respectively. Positive staining with epithelial specific Pan-cytokeratin (PanCK) and fibroblast specific protein (FSP-1) further confirmed their epithelial and fibroblast origin. Unique Short Tandem Repeat (STR) profile of the cultures confirmed their novelty, while the similarity of the STR profiles between the epithelial and fibroblast cells from the same patient, confirmed their autologous nature. DNA analysis revealed aneuploidy of the established cultures. An increase in the tumorigenic potential of the established epithelial cultures upon treatment with CAF-conditioned medium proved the 'CAF-ness' of the established fibroblast cells. The established cultures are the first of their kind which would serve as a useful platform in understanding the tumor-stroma cross-talk in tongue cancer progression.

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Section: Characterizationmentioning

confidence: 99%

Establishment and characterization of novel autologous pair cell lines from two Indian non‑habitual tongue carcinoma patients

et al. 2022

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“…In some countries, cross-sectional and cohort studies have shown an interesting trend in the female age-specific distribution of HPV, whereby there is a first peak at a younger age (r 25 years) and a clear second peak among individuals aged 45 years or older (Munoz et al, 2004;Herrero et al, 2005;Franceschi et al, 2006;De Sanjose et al, 2007;Smith et al, 2008;Asiaf et al, 2012). The rationale behind the first peak, which comes soon after the onset of sexual relations, is higher levels of sexual activity with multiple partners and a low viral immunity.…”

Section: Epidemiology Of Human Papillomavirus Infectionsmentioning

confidence: 99%

Review of the current knowledge on the epidemiology, pathogenesis, and prevention of human papillomavirus infection

Asiaf

Ahmad

Mohammad

et al. 2014

European Journal of Cancer Prevention

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Human papillomavirus (HPV) infection is a central and necessary, although not sufficient, cause of cervical cancer. Besides HPV, the additional multiple risk factors related with the onset of cervical cancer are early-age sexual activities; high number of sexual partners, which is the most salient risk factor; suppression and alteration of the immune status; long-term use of oral contraceptives; and other hormonal influences. The tumor-suppressor proteins p53 and pRb are degraded and destabilized through ubiquitination by viral oncoproteins E6 and E7. Over 95% of cervical cancer cases worldwide test positive for oncogenic HPV DNA. Although cervical screening procedures have been successful in reducing the disease burden associated with HPV infection because of lack of resources or inadequate infrastructure many countries have failed to reduce cervical cancer mortality. Therefore, prevention may be a valuable strategy for reducing the economic and disease burden of HPV infection. At present, two successful prophylactic HPV vaccines are available, quadrivalent (HPV16/18/6/11) 'Gardasil' and bivalent (HPV16/18) 'Cervarix' for vaccinating young adolescent girls at or before the onset of puberty. Recent data indicate that vaccination prevents the development of cervical lesions in women who have not already acquired the vaccine-specific HPV types. Moreover, several therapeutic vaccines that are protein/peptide-based, DNA-based, or cell-based are in clinical trials but are yet to establish their efficacy; these vaccines are likely to provide important future health benefits. The therapeutic vaccination mode of prevention is a promising area of research, as revealed in preclinical trials; however, clinical trials based on large populations are warranted before reaching a valid conclusion. This review summarizes the studies on the epidemiology of HPV infection, the pathogenesis of viral oncoproteins in the oncogenesis of cervical cancer, the economic and health burden of HPV-related diseases, and, finally, focuses on the results of recent clinical vaccination trials.

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“…In many studies, younger age was found to be associated with HPV infection. Asiaf A et al 8 found the highest prevalence of HPV infection in women below 30 years. Our study showed 57% positivity in the age group of <30 years.…”

Section: Discussionmentioning

confidence: 97%

Prevalence of Human Papilloma Virus and Its Associated Risk Factors in Women With Abnormal Cytology at a Tertiary Care Hospital of North India

Bhawna¹,

Singh²,

Oberoi³

et al. 2017

jemds

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BACKGROUNDCervical cancer is the fourth most common cancer worldwide. High-risk Human Papilloma Virus (HPV) infection is an important event in the progression of cervical cancer. The most common HPV types associated with pre-invasive and invasive cervical lesions are HPV 16, 18, 31, 33 and 45. As anogenital HPV infection is transmitted by sexual intercourse, sexual behaviour patterns among women and their partners often dictate women's risk for HPV infection and acquisition.Aim of this study was to know the prevalence of HPV and its associated risk factors in women with abnormal cytology. MATERIALS AND METHODSThe samples were processed for DNA extraction and finally amplification of this DNA was done with the help of PCR system using specific primers for the detection of HPV DNA. Study Design-We performed a prospective observational study on 50 clinical samples of cervical scrapings from women with abnormal cytology. RESULTSIn our study, prevalence of HPV came out to be 28 percentage. Early age at marriage, multiple sexual partners, use of OCPs, multiparity were found to be significant risk factors for HPV infection. CONCLUSIONOur study concluded that younger age at marriage, multiple sexual partners, multiparity, use of OCPs are the most important risk factors for the acquisition of HPV infection. -10-2017, Peer Review 01-11-2017, Acceptance 06-11-2017, Published 13-11-2017. Corresponding Author: Kanwardeep Singh, Associate Professor, Department of Microbiology, GMC, Amritsar. E-mail: kdmicrogmcasr@gmail.com DOI: 10.14260/jemds/2017/1341 This study provided an opportunity to investigate the determinants of HPV DNA along with its prevalence in women with abnormal cytology in relation to demographic, sexual and reproductive factors. KEYWORDS

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Prevalence of Human Papillomavirus Infection in a Kashmiri Ethnic Female Population

Cited by 10 publications

References 29 publications

Establishment and characterization of novel autologous pair cell lines from two Indian non‑habitual tongue carcinoma patients

Establishment and characterization of novel autologous pair cell lines from two Indian non‑habitual tongue carcinoma patients

Review of the current knowledge on the epidemiology, pathogenesis, and prevention of human papillomavirus infection

Prevalence of Human Papilloma Virus and Its Associated Risk Factors in Women With Abnormal Cytology at a Tertiary Care Hospital of North India

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