Prevalence of human papillomavirus in squamous cell carcinomas of the soft palate

Frandsen, Vibe Lyng; Larsen, Christian Grónhøj

doi:10.1136/jclinpath-2015-203081

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…Of note, all three soft palate tumors were HPV-related. The prevalence of HPV-related OPSCC located in the soft palate has not been well studied, however rates of 0-67% have been reported in the literature [11]. While it is established that HPVpositivity in the soft palate is generally lower than in other subsites, concomitant tobacco smoking likely increased those patients' risk of OPSCC.…”

Section: Discussionmentioning

confidence: 99%

“…DNA aliquot was then tested using EUROarray HPV kit (EuroImmun). The test is designed for molecular diagnostic in vitro detection and typing of 30 human anogenital high-risk and low-risk papilloma viruses (HPV 6,11,16,18,26,31,33,35,39,40,42,43,44,45,51,52,53,54,56,58,59,61,66,68,70,72,73,81,82,89) from DNA preparations. The test system is based on detection of the viral oncogenes E6/E7.…”

Section: Human Papillomavirus Testingmentioning

confidence: 99%

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HPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCR

Maroun

Feghali

Traboulsi

et al. 2020

Infect Agents Cancer

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Background: Given the paucity of data and widely variable rates that have been reported, the main objective of this study was to examine the prevalence of HPV-positivity in oropharyngeal squamous cell carcinoma (OPSCC) in Middle Eastern patients presenting to one of the region's largest tertiary care centers using polymerase chain reaction (PCR) amplification of the HPV E6/E7 oncogenes, a highly sensitive and specific method of detection. Methods: Medical charts and archived pathological specimens were obtained for patients diagnosed with biopsy proven oropharyngeal cancer who presented to the American University of Beirut Medical Center between 1972 and 2017. DNA was extracted from paraffin-embedded specimens and tested for 30 high-risk and low-risk papilloma viruses using the PCR-based EUROarray HPV kit (EuroImmun). Results: A total of 57 patients with oropharyngeal cancer were initially identified; only 34 met inclusion/exclusion criteria and were included in the present study. Most patients were males (73.5%) from Lebanon (79.4%). The most common primary tumor site was in the base of tongue (50%), followed by the tonsil (41.2%). The majority of patients (85.3%) tested positive for HPV DNA. Conclusion: The prevalence of HPV-positivity amongst Middle Eastern OPSCC patients, specifically those from Lebanon, may be far greater than previously thought. The Lebanese population and other neighboring Middle Eastern countries may require a more vigilant approach towards HPV detection and awareness. On an international level, further research is required to better elucidate non-classical mechanisms of HPV exposure and transmission.

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Section: Discussionmentioning

confidence: 99%

Section: Human Papillomavirus Testingmentioning

confidence: 99%

HPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCR

Maroun

Feghali

Traboulsi

et al. 2020

Infect Agents Cancer

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“…Misclassification of tumor site could account for the expression of CK7 within tumors that were designated clinically as non-tonsillar but were within the oropharynx and could have actually arisen from a tonsillar crypt source. Issues related to misclassification of oropharyngeal SCC are well-described in the literature (27,(48)(49)(50)(51)(52), and misclassification has even been described as a limitation in relation to site and CK7 expression before (53). Future investigation of CK7 expression in non-oropharyngeal tumors may help further characterize specificity to the tonsillar region.…”

Section: Discussionmentioning

confidence: 99%

Cytokeratin 7 in Oropharyngeal Squamous Cell Carcinoma: A Junctional Biomarker for Human Papillomavirus–Related Tumors

Woods

Keegan

White

et al. 2017

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Human papillomavirus (HPV)-related oropharyngeal squamous cell carcinoma (SCC) represents a distinct subgroup of head and neck tumors. We analyze the expression of cytokeratin 7, a junctional biomarker with a SEQIKA fragment, which stabilizes HPV-16 E7 transcripts, in oropharyngeal SCCs. Archived tumor specimens and epidemiologic data were collected from patients with oropharyngeal SCCs over 10 years. Briefly, DNA was extracted from tissue blocks, and HPV testing was carried out using SPF10 HPV PCR and INNO-LiPA HPV Genotyping. Immunohistochemical staining for CK7 and p16ink4a was performed on the Ventana BenchMark Ultra Immunostainer. Analysis was by light microscopy using the -score. CK7 expression was correlated with epidemiologic data, p16ink4a positivity, and HPV status using SPSS. CK7 expression was observed specifically and uniformly in the tonsillar crypt epithelium of normal tonsils and tumor specimens. There were 226 cases of oropharyngeal SCCs, with 70 demonstrating both HPV and p16 positivity. Of 216 cases evaluated for CK7, 106 demonstrated some positivity, whereas -score> 60 was seen in 55 of these. CK7 -score> 60 was significantly associated with tonsillar subsite and HPV and p16 positivity. An association between CK7 and HPV has been demonstrated. CK7-expressing tonsillar crypt cells potentially represent an oropharyngeal subsite susceptible to HPV-related SCC. Along with the cervix and anorectum, specific oropharyngeal expression of CK7 in a site predisposed to HPV-related tumors may suggest a role for CK7 in the pathogenesis of this subgroup of tumors. Further research is warranted to characterize the association between CK7 and HPV-related head and neck SCC. .

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“…La prevalencia de infección por VPH en el carcinoma de orofaringe varía desde el 4% al 93%. El biomarcador más utilizado para detectar presencia de VPH es el P16 y tinciones >70% se consideran positivas y muestran actividad del VPH [8]. La prevalencia media en el sur de Europa es del 17% aunque en España es discretamente inferior [9].…”

Section: Descripciónunclassified

Carcinoma de paladar blando. Descripción de una serie de casos

Zabaleta-López

Ramos-Casademont

Gómez-Tomé

et al. 2020

ORL

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Conflicto de intereses: Los autores declaran no tener conflictos de intereses Imágenes: Los autores declaran haber obtenido las imágenes con el permiso de los pacientes Política de derechos y autoarchivo: se permite el autoarchivo de la versión post-print (SHERPA/RoMEO) Licencia CC BY-NC-ND. Licencia Creative Commons Atribución-NoComercial-SinDerivar 4.0 Internacional Universidad de Salamanca. Su comercialización está sujeta al permiso del editor RESUMEN Introducción y objetivo: Describir las características clínicas, anatómicas y el tratamiento quirúrgico, incluyendo las distintas opciones de reconstrucción de los tumores de paladar blando. Descripción: Estudio retrospectivo de 6 pacientes diagnosticados y tratados en el servicio de Otorrinolaringología del hospital San Pedro de Logroño entre los años 2018 y 2019. Discusión: Exponemos los resultados de nuestros pacientes y realizamos una revisión de los tumores de orofaringe localizados en el paladar blando, los detalles anatómicos y técnicas quirúrgicas reconstructivas más utilizadas en el tratamiento quirúrgico de los tumores de paladar blando. Conclusiones: La prevalencia de los tumores de orofaringe ha aumentado en los últimos años a expensas de la infección por el virus del papiloma humano (VPH). Sin embargo, los carcinomas de paladar blando son mayoritariamente P16 negativos. Estos tumores son poco sintomáticos en fases iniciales y es necesario un alto índice de sospecha para evitar diagnósticos tardíos. El desarrollo de la cirugía transoral ha impulsado el tratamiento quirúrgico de los tumores de paladar blando y puede reducir la necesidad de tratamientos complementarios hasta en un 40% de los casos. La reconstrucción se hace necesaria en resecciones mayores del 50%. PALABRAS CLAVE Anatomía; carcinoma; orofaringe; paladar blando; procedimientos quirúrgicos reconstructivos SUMMARY Introduction and objective: To describe clinical and anatomical features, and surgical treatment including several techniques of reconstructive surgery of soft palate carcinomas. Description: We conducted a retrospective study with 5 patients. All of them were diagnosed and treated in our department during 2018 and 2019. Discussion: We present our results and make a review on soft palate tumors pointing out anatomical details and most employed reconstructive surgical techniques for soft palate carcinoma´s removal. Conclusions: The incidence of oropharyngeal carcinoma is increasing worldwide essentially due to increasing prevalence of human

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Prevalence of human papillomavirus in squamous cell carcinomas of the soft palate

Cited by 8 publications

References 22 publications

HPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCR

HPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCR

Cytokeratin 7 in Oropharyngeal Squamous Cell Carcinoma: A Junctional Biomarker for Human Papillomavirus–Related Tumors

Carcinoma de paladar blando. Descripción de una serie de casos

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