Prevalence of Human Papillomavirus (HPV) Infection and the Association with Survival in Saudi Patients with Head and Neck Squamous Cell Carcinoma

Al-Harbi, Najla; Judia, Sara Bin; Al-Qahtani, Wejdan; Khoja, Hatim; El-Sebaie, Medhat; Tulbah, Asma

doi:10.3390/cancers11060820

Cited by 37 publications

(40 citation statements)

References 48 publications

(65 reference statements)

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“…Our data are in line with above observations and further confirm the roles of AKT and ERK/MAPK signaling pathways in regulating TROP2 expression in HNSCC. According to previous studies, tumor stage is an important prognostic factor for overall survival of head and neck cancers [34,35], however, in our study, the prognostic values of T category for both OS and DFS did not show the significant differences. It is possible that the difference may be due to our relative small sample size, and clearly, further studies are required to make this observation more robust.…”

Section: Discussioncontrasting

confidence: 89%

miR-488-3p sponged by circ-0000495 and mediated upregulation of TROP2 in head and neck squamous cell carcinoma development

Yu¹,

Zhang²,

Guo³

et al. 2020

J. Cancer

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TROP2 (trophoblast cell surface antigen 2) overexpression has been reported in many human cancers. The correlation between TROP2 and tumor aggressiveness has implied it could be a prognostic indicator. However, the roles of TROP2 and their underlying mechanisms remain of great interest in head and neck squamous cell carcinoma (HNSCC) biology. In the current study, the prognostic significance of TROP2 in HNSCC archival samples was determined using immunohistochemistry. Quantitative reverse transcriptase PCR (qRT-PCR) was used to measure the phenotypic effects of TROP2 knockdown, miR-488-3p re-expression, and circRNAs expression. Cell viability, migration/invasion as well as in vivo tumor formation assays were accessed. The interactions of miRNAs-TROP2 or circRNAs-miRNAs were determined by qRT-PCR, western blot analysis and luciferase assays. TROP2 was demonstrated overexpression in HNSCC patients and cancer cell lines. High expression of TROP2 was significantly associated with patient relapse. TROP2 promoted tumor cell proliferation, migration, invasion, and tumor growth, through AKT and MAPK pathways. Further investigation revealed that TROP2 is a direct target of miR-488-3p, while circ-0000495 bounds to miR-488-3p. Our study unraveled a novel mechanism by which down-regulation of miR-488-3p sponged by circ-0000495 releases its epigenetic silencing to TROP2. The increased TROP2 promotes tumor proliferation, therefore, providing evidence in support of targeting the circ-0000495/miR-488-3p/TROP2 axis in contributing to HNSCC therapy and preventing tumor metastasis.

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Section: Discussioncontrasting

confidence: 89%

miR-488-3p sponged by circ-0000495 and mediated upregulation of TROP2 in head and neck squamous cell carcinoma development

Yu¹,

Zhang²,

Guo³

et al. 2020

J. Cancer

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“…prevalence of HPV DNA among the studied cases (6/28 oropharyngeal and 4/257 Oral cavity cancers) [15].…”

Section: Discussionmentioning

confidence: 90%

Prevalence of HPV Infection in Head and Neck Cancer Patients in Egypt: National Cancer Institute Experience.

Salem¹,

Bahaa

Rouby

et al. 2020

Preprint

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Background: The new classification of Head and Neck Squamous Cell Carcinoma (HNSCC) according to their association with human papillomavirus (HPV) infection into HPV positive tumors with good prognosis and HPV negative tumors with poor prognosis has changed the treatment strategy of HNSCCs dramatically worldwide. However, data concerning HPV infection in HNSCCs patients is still limited and there are no studies regarding HPV genotypes in HNSCC among Egyptians. Therefore, the aim of the current study is to investigate the frequency of HPV and to detect the most prevalent HPV genotypes in the patients with oral cavity and oropharyngeal carcinoma from EgyptMethods: This cross sectional study was conducted from January 2016 to November 2017. Ninety-two oral brushing samples (76 oral cavity and 16 oropharynx) were collected by cytobrushing in CYTO-fast solution. HPV-DNA was subjected to two methods which are the: (1) HPV Direct Flow CHIP system for automatic detection and genotyping (2) conventional PCR for detection of HPV using consensus primers (MYO9/MYO11). SPSS software was used for statistical analysis.Results: Out of the 92 cases assessed, HPV DNA was present in three samples only (3/92; 3.3%) and the HPV66 genotype was the only genotype detected by HPV Direct Flow CHIP System.Conclusion: The Results of this study may (1) indicate that the HPV-associated HNSCC among Egyptian patients is very low and (2) highlight the presence of HPV66 genotype in these malignancies. Other studies from other countries in general and from different governorates in Egypt are still needed to determine the exact frequency of HPV infections in head and neck cancer.

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“…Around 600,000 people worldwide become afflicted with a head and neck carcinoma (HNC) per year [1], with squamous cell carcinoma (HNSCC) constituting over 90% of these cases [2]. HNSCC has been characteristically linked to risk determinants, e.g., alcohol intake and smoking.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic and prognostic impact of E6/E7 mRNA compared to HPV DNA and p16 expression in head and neck cancers: an Egyptian study

Dwedar

Omar

Eissa

et al. 2020

Alexandria Journal of Medicine

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Introduction: Human papillomavirus (HPV) is identified as a culprit in a subset of head and neck squamous cell carcinomas (HNSCCs). The clinicopathologic profile displayed by this subset diverges from that of HPV-negative HNSCCs. Despite a variety of available tests, there is no consensus on which technique is the best for detection of HPV in HNSCCs. Although this field has received substantial interest within different continents, African and Egyptian populations are not yet well studied within the literature. Methods: This cross-sectional study was carried out to detect HPV prevalence in HNSSC and to correlate the viral prevalence with different clinicopathologic parameters as well as with the patients' outcome. For 51 patients with HNSCC, HPV-16 DNA was determined via PCR, while E6/ E7 mRNA was detected employing real-time PCR. Immunohistochemistry (IHC) was performed to assess p16 status. Results: P16 was overexpressed in 49% of cases, while HPV-16 DNA was detected in 52.9% of cases, and likewise, E6/E7 mRNA was found in 52.9% of cases. There was a very good agreement between HPV16 DNA and RNA results (κ = 0.843, P-value <0.001). Meanwhile, a good agreement was revealed between HPV16 DNA and p16 IHC results (κ = 0.608, P-value <0.001). Similarly, there was a good agreement between HPV RNA results and p16 IHC results (κ = 0.608, P-value <0.001). By the end of the study period, 13.7% of the enrolled patients died, with the overall survival of the studied patients being 17.29 months. Of note, there was no statistically significant correlation between the overall survival and HPV status. Conclusion: The present study highlights the significant role played by HPV in HNSCC. Furthermore, it reveals that although p16 has been a marker of HPV existence in HNSCC, it should not be the sole determinant of HPV role in tumorigenesis.

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Prevalence of Human Papillomavirus (HPV) Infection and the Association with Survival in Saudi Patients with Head and Neck Squamous Cell Carcinoma

Cited by 37 publications

References 48 publications

miR-488-3p sponged by circ-0000495 and mediated upregulation of TROP2 in head and neck squamous cell carcinoma development

miR-488-3p sponged by circ-0000495 and mediated upregulation of TROP2 in head and neck squamous cell carcinoma development

Prevalence of HPV Infection in Head and Neck Cancer Patients in Egypt: National Cancer Institute Experience.

Diagnostic and prognostic impact of E6/E7 mRNA compared to HPV DNA and p16 expression in head and neck cancers: an Egyptian study

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