Prevalence of human papilloma virus (HPV) and its genotypes in cervical specimens of Egyptian women by linear array HPV genotyping test

Youssef, Mohamed Ahmed; Abdelsalam, Lobna; Harfoush, Reem Abdelhameed; Talaat, Iman M.; Elkattan, Eman; Mohey, Abeer; Abdella, Rana; Salah, Mona; Foad, Hany Ahmed; Elsayed, Ahmed; Elkinaai, Naglaa; Ghaith, Doaa; Rashed, Mohamed E.; Ghafar, Mohamed Abd-El; Khamis, Y.; Hosni, Ahmed Naguib

doi:10.1186/s13027-016-0053-1

Cited by 24 publications

(23 citation statements)

References 36 publications

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“…In Turkey, three studies showed an overall HPV prevalence ranging from 13.7% to 52% in women with abnormal cytology [42–44], with HR-HPV types detected in 27% and 20% of L-SIL and H-SIL cases [42]. In contrast, other studies in the surrounding region reported higher HPV prevalence rates in women with abnormal cytology, such as 84% in Jordan [45] and 100% in Egypt [46]. In the Jordanian study, HR-HPV prevalence was 72.2% and 78.6% of L-SIL and H-SIL cases, respectively [45].…”

Section: Discussionmentioning

confidence: 99%

HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities

2017

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BackgroundHuman papillomavirus (HPV) is the most common sexually transmitted agent, and it can cause cervical lesions and cancer in females. Currently, information regarding the prevalence of HPV in Cyprus is lacking. The aim of this study was to evaluate the HPV type-specific prevalence in 596 women, aged 19–65 years, with cytological abnormalities. Additionally, in a subset of 348 women for whom cytology results of the Pap test were available, the association between HPV infection and cervical disease was investigated.MethodsHPV detection and typing was carried out using PCR and restriction fragment length polymorphism analysis, respectively.ResultsOverall, the HPV prevalence was 72.8%, and it was shown to be age dependent, with a decreasing prevalence until the age of 45 years (p = 0.0018, χ2). Two hundred and fifty-eight women (59.4%) were infected with high-risk HPV, 151 (34.8%) with low-risk HPV, and 25 (5.8%) with HPV types of unknown risk. The most common high-risk HPV type was HPV16 (17.7%), followed by HPV31 (12.9%), HPV58 (7.1%), HPV68 (4.6%), HPV18 (4.1%), and HPV56 (3.7%). Among the women for whom cytology results were available, 268 (77%) were HPV positive, with a sample distribution as follows: 188 (74%) had atypical squamous cells of undetermined significance (ASCUS), 61 (85.9%) had low-grade squamous intraepithelial lesion (L-SIL), and 19 (82.6%) had high-grade squamous intraepithelial lesion (H-SIL). HPV16 was the most common type among women affected by L-SIL (19.7%) and H-SIL (15.8%), with HPV31 being the most common type in women affected by ASCUS (16.5%).ConclusionsThe present study provides the first epidemiological data related to HPV prevalence and type distribution in Cypriot women with cytological abnormalities.

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Section: Discussionmentioning

confidence: 99%

HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities

2017

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“…There was no HPV62 detection in samples without cervical lesions in both populations. Among LR-HPV types detected in the Northern Indian population, HPV62 was the most common (10.5% of HPV positive samples) and in Egypt HPV62 was also the most prevalent LR-HPV among HPV positive women (17.4 and 9.7% in two different research works) [26–28]. In Thai women, HPV62 is also among the most frequent LR-HPV (11.3% of HPV positive samples) [29].…”

Section: Discussionmentioning

confidence: 99%

Genetic variability in E6, E7 and L1 genes of Human Papillomavirus 62 and its prevalence in Mexico

Artaza‐Irigaray

Flores-Miramontes

Olszewski

et al. 2017

Infect Agents Cancer

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BackgroundHuman papillomavirus (HPV) is the main etiological agent of cervical cancer, the third most common cancer among women globally and the second most frequent in Mexico. Persistent infection with high-risk HPV genotypes is associated with premalignant lesions and cervical cancer development. HPVs considered as low risk or not yet classified, are often found in coinfection with different HPV genotypes. Indeed, HPV62 is one of the most prevalent HPV detected in some countries, but there is limited information about its prevalence in other regions and there are no HPV62 variants currently described. The aim of this study was to determine the prevalence of HPV62 in cervical samples from Mexican women and to identify mutations in the L1, E6 and E7 genes, which have never been reported in our population.MethodsHPV screening was performed by Cobas HPV Test in women who attended prevention health programs and dysplasia clinics. All HPV positive samples (n = 491) and 87 additional cervical cancer samples were then genotyped with Linear Array HPV Genotyping test. Some samples were selected to corroborate genotyping by Next-Generation sequencing. On the other hand, nucleotide changes in L1, E6 and E7 genes were determined using PCR, Sanger sequencing and analysis with the CLC-MainWorkbench 7.6.1 software. L1 protein structure was predicted with the I-TASSER server.ResultsUsing Linear Array, HPV62 prevalence was 7.6% in general population, 8% in Cervical Intraepithelial Neoplasia grade 1 (CIN1) samples and 4.6% in cervical samples. The presence of HPV62 was confirmed with Next-Generation sequencing. Regarding L1 gene, novel sequence variations were detected, but they did not alter the tertiary structure of the protein. Moreover, several nucleotide substitutions were found in E6 and E7 genes compared to reference HPV62 genomic sequence. Specifically, three non-synonymous sequence variations were detected, two in E6 and one in E7.ConclusionsHPV62 is a frequent HPV genotype found mainly in general population and in women with CIN1, and in 90.5% of the cases it was found in coinfection with other HPVs. Novel nucleotide changes in its L1, E6 and E7 genes were detected, some of them lead to changes in the protein sequence.

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“…HPV, according to the type, is responsible for the formation of benign lesions (warts or papillomas) and intraepithelial neoplasia, which in combination with specific and close-related risk factors, similar to those of cervical cancer, such as life style factors, multiple sexual partners, and infectious agents may lead to carcinogenesis [ 4 , 5 ]. HPV 16, 18, and 31 genotypes were the most prevalent high risk (HR) HPV types, while HPV 6 and 62 were the most prevalent low risk (LR) types in a group of 152 women who participated in a study investigating the relationship between HPV and cervical cancer in Egypt [ 6 ]. The HPV 52 and HPV 16, 58, 39, and 51 demonstrated the HP-HPV infections, while LR-HPV was HPV 81 followed by HPVs 6, 11, and 44 in a study investigating the correlation between HPV genotypes and cervical carcinoma [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Human Papilloma Virus (HPV) and Fertilization: A Mini Review

et al. 2018

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Human papilloma virus (HPV) is one of the most prevalent viral sexually transmitted diseases. The ability of HPV to induce malignancy in the anogenital tract and stomato-pharyngeal cavity is well documented. Moreover, HPV infection may also affect reproductive health and fertility. Although, the impact of HPV on female fertility has not been thoroughly studied it has been found also to have an impact on semen parameters. Relative information can be obtained from studies investigating the relationship between HPV and pregnancy success. Furthermore, there is an ongoing debate whether HPV alters the efficacy of assisted reproductive technologies. An association between HPV and assisted reproductive technologies (ART) programs has been reported. Nevertheless, due to conflicting data and the small number of existing studies further research is required. It remains to be clarified whether HPV detection and genotyping could be included in the diagnostic procedures in couples undergoing in vitro fertilization (IVF)/intrauterine insemination (IUI) treatments. Vaccination of both genders against HPV can reduce the prevalence of HPV infection and eliminate its implications on human fertility. The aim of the present mini-review is to reiterate the association between HPV and human fertility through a systematic literature review.

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Prevalence of human papilloma virus (HPV) and its genotypes in cervical specimens of Egyptian women by linear array HPV genotyping test

Cited by 24 publications

References 36 publications

HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities

HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities

Genetic variability in E6, E7 and L1 genes of Human Papillomavirus 62 and its prevalence in Mexico

Human Papilloma Virus (HPV) and Fertilization: A Mini Review

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