Prevalence of human papilloma virus in marginal periodontium and its association with periodontitis: A cross sectional study

Abstract

Context:Bacterial pathogens in dental plaque are necessary for the development of periodontitis but this etiology alone does not explain all its clinicopathologic features. Researchers have proven the role of certain viruses like herpes virus in periodontal disease which implies that other viral agents like human papilloma virus may also be involved.Aims:This cross-sectional study was conducted to determine the proportion of patients with human papilloma virus (HPV-16) in marginal periodontium by analyzing DNA from the gingival tissue sample and to understand its association with periodontitis.Settings and Design:102 systemically healthy patients between the age group of 15 and 70 years reporting to the Department of Periodontology who required surgical intervention (flap surgery for patients with periodontitis and crown lengthening for healthy patients) with internal bevel gingivectomy were selected.Materials and Methods:After scaling and root planning, gingival tissue was collected during the respective surgical procedure. DNA was isolated and amplified using specific primers for HPV-16 by polymerase chain reaction (PCR). The amplified products were checked by agarose gel electrophoresis.Results:No HPV DNA was detected in the 102 samples analyzed.Conclusion:Marginal periodontium does not contain HPV in this study population and hence there was no association between HPV and periodontitis.

A Pilot Study into the Association between Oral Health Status and Human Papillomavirus—16 Infection

“…The oral cavity and oropharynx are lined with various types of specialised epithelia (i.e., mucosal squamous epithelium) [22]. Due to HPV’s known basal cell tropism, it is logical to examine the epithelial components within these regions when studying HPV infections [22].…”

“…Due to HPV’s known basal cell tropism, it is logical to examine the epithelial components within these regions when studying HPV infections [22]. Previous studies have shown that self-reported poor oral hygiene is an independent risk factor for high-risk HPV infections [15,23].…”

High risk human papillomavirus in the periodontium : A case control study

“…In this study, of the total of 90 samples, 38 samples (42.3%) of the samples were found to contain HPV E6/E7 mRNA (0%–2% or >2%), this is in contrast to study by Jacob et al .,[ 18 ] the results obtained showed no HPV was detected in the 102 samples analyzed, where the method of analysis used was PCR.…”

“…Previously, HPV DNA has been detected using polymerase chain reaction among other methods. [ 8 17 18 ] However, there is evidence that E6/E7mRNA testing is more specific than DNA testing for HPV detection. [ 19 ] (The HPV genome is composed of three major regions: the long control region, the early genes E1–E8 and late genes L1–L2.…”

Association of Epstein-Barr Virus (Ebv) but Not Human Papillomavirus (Hpv) With Gingivitis and/or Periodontitis in Transplanted Individuals

“…Jacob et al . 13 studying healthy individuals with and without periodontal disease did not find any positive gingival tissue for HPV-16. These results were similar to those obtained by Horewicz et al 14 , with no detection of HPV-16 in either periodontitis or gingivitis.…”