Prevalence of High risk Human Papillomavirus in cervical dysplasia and cancer samples from twin cities in Pakistan

Gul, Sana; Murad, Sheeba; Javed, Aneela

doi:10.1016/j.ijid.2015.02.018

Cited by 35 publications

(26 citation statements)

References 28 publications

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“…These also agree with the findings of Bosch et al, 18 Clifford et al, 19 Dienne et al, 20 and Szostek et al 21 It has also been observed that 88.3% of abnormal cytology test results were positive for the viral DNA. This conforms to reports of Gargiulo et al, 22 Gul et al 23 and slightly lower than that of Chen et al 24 who reported 92.9% in patients with CIN2+ lesions. But in contrast with what was found by Piras et al, 25 Thomas et al 13 and Zhang et al 26 However, the differences could be explained by larger samples and methodologies employed by the three researchers.…”

Section: Discussionsupporting

confidence: 92%

Molecular detection of HPV 16/18 E6 genes from cervical cells

Auwal

Rogo

2015

Int J Res Med Sci

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INTRODUCTIONHuman papillomaviruses (especially types 16 and 18) are the causative agents of cervical cancer, 1 the second leading female cancer in the world; the most frequently reported cancer in most developing countries and the principal cause of mortality from cancer in general. The virus encodes for 2 oncoproteins E6 and E7 which directly contribute towards initiation and maintenance of tumour development.2 Protein products of these genes interfere with the normal function of tumour suppressor genes. E7 binds and inactivate retinoblastoma protein RB while E6 binds P 53 protein and directs its degradation leading to resistance to apoptosis, uncensored cell growth and malignant transformation. 3 E6 is of particular interest because of its multiple roles in the cell and in interacting with many other proteins. It reacts with E6-AP and form an ubiquitin-protein ligase a process leading to degradation of the P 53 gene. 4 It also targets NFX1-9 cellular protein which normally represses reduction in telomerase (a protein that make cells to divide unlimited times) and consequently keep cell growth uncheck.5 E6 acts as a translational co-factor, specifically transcription activator when interacting with cellular transcription factor E2F/DP1. The interaction of E6 with membrane associated granulocytes kinase family (MAGUK) protein ABSTRACT Background: Epidemiological, clinical and molecular studies have established the link between genital infection with high risk human papillomavirus (HPV) and cervical cancer but there is great challenge in establishing early infection by both clinicians and the laboratories. The virus cannot be grown in conventional cell cultures and serology cannot different between active and past infection. Molecular studies remain the goal standard as it detects viral nucleic acid or cellular antigens indicative of oncogenic potential in cytology or biopsy specimen. The study was aimed to molecularly determine the presence of HPV 16/18 and expression of E6 gene in squamous intraepithelial lesions. Methods: Cervical cells were collected from 18 women with positive cytology test results and 32 controls in gynaecology clinic of Murtala Muhammad Specialist Hospital, Kano Nigeria and HPV 16/18 were detected with E6 gene specific PCR primers. Results: Overall, HPV E6 gene was found in 76% of the women, 88.3% of positive cytology specimens and 71.2% controls. Conclusions: There is very high prevalence of HPV infection. The presence of HPV 16/18 E6 genes in cervical intraepithelial lesions may serve as a useful predictor of diagnosis and possible clinical outcome of the disease.

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Section: Discussionsupporting

confidence: 92%

Molecular detection of HPV 16/18 E6 genes from cervical cells

Auwal

Rogo

2015

Int J Res Med Sci

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“…HPV is small DNA virus commonly infecting mucosa and cutaneous keratinocytes. Up to now, at least 200 HPV genotypes are known [2]. HPV was assumed to be responsible of more than 99% of the cases of cervical cancer worldwide [3].…”

Section: Introductionmentioning

confidence: 99%

“…HPV subtypes have been divided into high-and low-risk on the basis of their oncogenic potential. HR-HPV subtypes are considered to be the leading etiological factors for cervical cancer [2][3][4]. According to the International Agency for Research on Cancer (IARC), the HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 66, regarded as HR-HPV.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2017

JDHODT

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“…HPV as a DNA tumor virus frequently causes epithelial proliferation and establishment of infections in the keratinocytes of the skin and mucosal surfaces (1,2). Currently, HPV is known as one of the most common causes of sexually transmitted infections worldwide (3). So far, more than 100 HPV types have been identified, of which approximately 30 -40 viral types are commonly associated with genital tract infections (4).…”

Section: Introductionmentioning

confidence: 99%

“…High-risk HPV types, such as HPV 16, 18, 31, 33, and 35, are mostly associated with cancer (5). HPV 16 and 18 are the most prevalent types reported worldwide, contributing to approximately 70% of cervical cancers, while other high-risk HPV genotypes are associated with more than 20% of cervical cancers globally (3,6). These epidemiologic findings indicate the role of certain HPV types in cervical cancer development (7).…”

Section: Introductionmentioning

confidence: 99%

Seroepidemiological Evaluation of High-Risk Human Papillomavirus Types Among Married and Unmarried Iranian Women in Tehran, Iran

Abedini

Karimi

Shamsy

et al. 2016

Arch Pediatr Infect Dis

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Background: Human papillomavirus (HPV) is a DNA virus that establishes productive infections only in keratinocytes of the skin or mucous membranes. Objectives: This study aimed to determine the frequency of two high-risk genotypes of HPV among married and unmarried Iranian women. Materials and Methods:This cross-sectional population-based study consisted of two groups of women: non-married girls referred for premarital counseling, and married women referred for pregnancy diagnosis. Blood samples were evaluated serologically with the ELISA method for HPV types 16 and 18. Results: A total of 177 women (89 single and 88 married) were tested for HPV strains. The frequency of HPV type 16 in single women was significantly higher than in married women (66.3% vs. 40.9%, P < 0.001). The relative frequency of HPV type 18 was also significantly higher in single women than in married women (62.9% vs. 47.7%, P < 0.001). Moreover, HPV type 16 and 18 positivity was significantly associated with higher education levels in single women compared to married women (P < 0.001). Classification of HPV-infected women according to age revealed that the frequency of HPV type 16 was significantly higher in single women aged 25 -35 years compared to married women (P < 0.05). Conclusions:The results showed remarkable rates of high-risk HPV types (16 and 18) in the studied population, which can be a serious alert for public health. This result highlights the potential impact of prophylactic vaccines for future protection against high-risk HPV types in Iranian women.

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Prevalence of High risk Human Papillomavirus in cervical dysplasia and cancer samples from twin cities in Pakistan

Cited by 35 publications

References 28 publications

Molecular detection of HPV 16/18 E6 genes from cervical cells

Molecular detection of HPV 16/18 E6 genes from cervical cells

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Seroepidemiological Evaluation of High-Risk Human Papillomavirus Types Among Married and Unmarried Iranian Women in Tehran, Iran

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