Prevalence of Hepatitis B Virus Genotype D in Precore Mutants Among Chronic Liver Disease Patients from New Delhi, India

Kar, Premashis; Polipalli, Sunil Kumar; Chattopadhyay, Saket; Hussain, Zahid; Malik, Abdul; Husain, Saddam; Medhi, Subhash; Begum, Naheeda

doi:10.1007/s10620-006-9625-z

Cited by 6 publications

(6 citation statements)

References 29 publications

(29 reference statements)

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“…However, some recent studies have suggested that acqui- sition of 1896A might contribute to the inactivation of the liver disease [40,46] , which is also supported by the findings of the present study. A low frequency of the 1896A mutation was detected in this part, in accordance with previous studies [29,[46][47][48] . Nevertheless, considering the prevalence of 1762T/1764A double mutations, this study suggests the potential of genotypes HBV/A and HBV/C in causing HBV-related disease severity in this part of India.…”

Section: Discussionsupporting

confidence: 92%

Molecular Epidemiology and Clinical Significance of Hepatitis B Virus Genotypes, Core Promoter and Precore Mutations in Eastern India

Datta

Biswas²,

Chandra³

et al. 2008

Intervirology

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Objectives: This unmatched case-control study aimed at determining the molecular epidemiology and clinical significance of HBV genotypes, core promoter (CP) and precore (PC) mutations in Eastern India. Methods: Serological, biochemical and molecular assays were used to examine antigens, ALT, genotypes, mutations and viremia among 106 inactive carriers and 183 chronic liver disease (CLD) patients. Results: Male gender (p < 0.001), HBeAg positivity (p = 0.050), high ALT (p < 0.001), high viremia (p < 0.001), CP mutations (p < 0.001), and genotypes A (p < 0.001) and C (p = 0.027) were significantly associated with CLD. Subjects infected with genotypes A and C had significantly higher prevalence of BCP mutations (p < 0.001), and low incidence of PC mutation (p < 0.001 and p = 0.047, respectively). Prevalence of genotype D was significantly higher among subjects with history of familial/childhood jaundice, while genotypes A and C were frequent among subjects with possible percutaneous exposure. Conclusions: Significant differences in risk factors and disease manifestation do exist among patients infected with different HBV genotypes. Genotypes A and C are frequently found among chronic liver disease patients, while genotype D is associated with inactive HBeAg-negative infections. This evaluation of clinical relevance of HBV genotypes, mutations and risk factors may be useful in disease prognosis, management and prevention strategies.

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Section: Discussionsupporting

confidence: 92%

Molecular Epidemiology and Clinical Significance of Hepatitis B Virus Genotypes, Core Promoter and Precore Mutations in Eastern India

Datta

Biswas²,

Chandra³

et al. 2008

Intervirology

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“…But, we did not observe genotype E in our population as the study of Singh et al, 23 in accordance with the study of Kar et al 24 Chattopadhyay et al 25 reported predominance of genotype D followed by genotype A among patients with chronic liver disease from New Delhi.…”

Section: Conflicts Of Interestsupporting

confidence: 86%

Prevalence of Genotype D and Precore/Core Promoter Mutations in Hepatitis B Virus-infected Population of North India

Kumar¹,

Pahal²,

Singh³

2011

Journal of Clinical and Experimental Hepatology

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Hepatitis B virus (HBV) isolates (21) from Punjab (North India) were studied for genotype distribution and precore/core promoter mutations. Assays of alanine aminotransaminase (ALT) and HBeAg were performed in all isolates. Genotypes were determined in all the samples by restriction fragment length polymorphism and the precore/core promoter mutations were studied by amplification and by direct sequencing of precore/core promoter region. Sixty-two percent of the isolates had higher ALT levels and 57% of the isolates were HBeAg negative. It was observed that 90% of the isolates were HBV D genotype (subgenotype D1 and D2) and 10% of the isolates were HBV A genotype (subgenotype A1). Amplification and sequencing of the precore/core promoter region showed

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“…Genotypes B and C are highly prevalent in Asia [7][8][9][10]. Genotype D has been found worldwide [11], but is predominant in the Mediterranean region, the Middle East, and South Asia [12][13][14][15]. Genotype E is restricted almost entirely to West Africa [16,17], and genotype F is found in Central and South America [18,19].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Hepatitis B virus genotype D in females in Karachi, Pakistan

Noorali

Hakim

McLean

et al. 2008

J Infect Dev Ctries

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Background: Hepatitis B virus (HBV) is an etiological agent of acute and chronic liver disease existing throughout the world. The high genetic variability of HBV genome is reflected by eight genotypes (A to H), and each genotype has characteristic geographical distribution, which is important epidemiologically. Previous studies from the province of Sindh, Pakistan, have reported that genotypes A and D as prevalent HBV genotypes. The aim of the study was to investigate the prevalence of HBV genotypes in physically healthy females at two universities in Karachi, Sindh, Pakistan. Methodology: Blood was collected from a total of 4,000 healthy female volunteer students and serum samples obtained were screened for Hepatitis B surface antigen (HBsAg), and anti-HBs antibodies by immunochromatography and ELISA. Genotyping was conducted for 6 HBV genotypes (A through F). Both genotyping and sequencing data of HBV positive females are described. Results: Out of 4,000 volunteers, 180 (4.5%) tested positive for HBsAg and 20 (0.5%) were positive for HBs antibodies. All 180 serum samples were genotyped by PCR and sequencing analyses was conducted for 21 samples. Out of 180 HBsAg positive samples, 150 showed a single HBV D genotype infection; 29 showed co-infection of genotypes B and D; and 1 exhibited co-infection of genotypes C and D. Twenty-one representative samples were selected randomly from genotypes B, D, and C for sequencing and each isolate clustered with respective reference genotype sequence, thus validating the genotyping strategy. Conclusion: Genotype D appears to be the dominant genotype prevalent in Karachi's otherwise healthy female population.

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Prevalence of Hepatitis B Virus Genotype D in Precore Mutants Among Chronic Liver Disease Patients from New Delhi, India

Cited by 6 publications

References 29 publications

Molecular Epidemiology and Clinical Significance of Hepatitis B Virus Genotypes, Core Promoter and Precore Mutations in Eastern India

Molecular Epidemiology and Clinical Significance of Hepatitis B Virus Genotypes, Core Promoter and Precore Mutations in Eastern India

Prevalence of Genotype D and Precore/Core Promoter Mutations in Hepatitis B Virus-infected Population of North India

Prevalence of Hepatitis B virus genotype D in females in Karachi, Pakistan

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