Prevalence of HCV-RNA-positive blood donors and correlation to ELISA and RIBA status

Cardoso, M. da Silva; Koerner, K.; Epple, S.; Kubanek, B

doi:10.1007/bf01697626

Cited by 20 publications

(9 citation statements)

References 23 publications

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“…Our data confirmed the rather low predictive value of positive second-generation EIAs previously reported by several investigators [7,9,10,19], Indeed, 27^10% of blood donor samples selected with Ab bott 2.0 EIA were found negative with Ortho, Sanofi and/or Murex 'third-generation EIAs. The conflicting results con cerned sera possessing weaker EIA ratio (< 3.0) in the sec ond-generation Abbott system.…”

Section: Discussionsupporting

confidence: 78%

“…The RT-PCR method was applied to improve the quality of HCV diagnosis especially in low-risk populations [9,16,23]. Our data indicate that the selected blood donors constitute a het erogeneous group in regard to HCV viremia: 31.9% of them had detectable amounts of viral RNA.…”

Section: Discussionmentioning

confidence: 90%

“…Although an immunoblot indeter minate result in blood donors was strongly associated with the absence of circulating HCV RNA, some samples have been shown by others [8,9,19,25,26] to be infectious and should be excluded from blood donation. In our study, such profile was found in 3/15 PCR-positive sera: 2 were indeter minate (core antigen) in the 3 tests, 1 was indeterminate (NS3 antigen) with 2 assays but positive with Riba 3 assay (NS3, NS4 antigens).…”

Section: Hcv Core Igm In Blood Donorsmentioning

confidence: 99%

“…The first-generation EIAs, based on a 'nonstructural' vi ral antigen showed moderate sensitivity and specificity, par ticularly in low-risk populations such as volunteer blood do nors [4][5][6][7], The introduction of structural and additional nonstructural antigens in second-generation EIAs improved the sensitivity of detection [7,8] although the question of specificity remains open [7,9,10]. Supplemental immuno blot assays were shown to predict HCV infection better than KARGER.…”

Section: Introductionmentioning

confidence: 99%

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HCV-lnfection in Blood Donors: Association between Anti-HCV Core IgM Antibodies and Serum HCV RNA

Ounanian-Paraz¹,

Morel-Baccard²,

Barlet³

et al. 1996

Vox Sanguinis

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Among 47 blood donors tested positive with HCV EIA 2.0 Abbott, 27 (57.4%) also reacted with four ‘third-generation’ EIAs. The presence of anti-HCV antibodies was confirmed with 3 different immunoblot assays in 16 of 27 sera (34.0%) while 10 samples (21.3%) had indeterminate profile with antibodies usually directed against structural core antigen. Anti-HCV core IgM response was found in 12 of 47 sera (25.5%) and HCV viremia detected by the polymerase chain reaction (PCR) procedure was observed in 15 samples (31.9%). A comparative study of the different markers confirmed a good correlation between a strong antibody response in EIAs and immunoblot assays and the presence of HCV RNA in the serum; only 2 immunoblot indeterminate samples were PCR positive. An association was observed between IgM antibodies against ‘core’ epitopes and HCV RNA carriage: all IgM-positive sera were found positive by PCR. However, the direct detection of viral genome remains the best method for identifying HCV carriers in the blood donor population.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 90%

Section: Hcv Core Igm In Blood Donorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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HCV-lnfection in Blood Donors: Association between Anti-HCV Core IgM Antibodies and Serum HCV RNA

Ounanian-Paraz¹,

Morel-Baccard²,

Barlet³

et al. 1996

Vox Sanguinis

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“…The prevalence of antibodies to the hepatitis C virus (HCV) worldwide is relatively high, ranging from 0.4% in Central Europe to over 10% in many countries in Africa [1,2]. HCV infection is mostly parentally acquired, and in the great majority of cases infection proceeds to chronicity.…”

Section: Introductionmentioning

confidence: 99%

Identification of Infection Routes for HCV in Blood Donors from Germany

Cardoso¹,

Körner²,

Koerner³

et al. 1998

Transfus Med Hemother

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Background: The objective of this study was to assess the relevant routes of infection for hepatitis C virus in blood donors from Baden-Württemberg (Germany). Participants and Methods: For this purpose an epidemiological questionnaire was elaborated and sent to 528 blood donors. One half of these donors had an anti-HCV-positive status confirmed by supplemental test (case group); the other half comprised blood donors who were seronegative for all infectious markers (control group). 72% of the enrolled donors (n = 380) sent back the answered questionnaire for evaluation. Results: From this investigation we could identify three major routes of HCV infection: a previous history of blood transfusion (p < 0.05), drug abuse (p < 0.05) and, in male participants, tatooing (p < 0.05). Seropositivity to HCV was significantly related to episodes of hepatitis in adult age (p < 0.05). Conclusion: Additional approaches to the usual preprinted donor’s questionnaire are needed, particularly to inquire about drug abuse and tatooing, since the established procedure seems to be quite inefficient.

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Hepatitis C virus RNA and liver histology in blood donors reactive to a single antigen by second-generation recombinant immunoblot assay

et al. 1995

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The clinical significance of single band reactivity (indeterminate pattern) at anti-hepatitis C virus (HCV) second-generation recombinant immunoblot assay (RIBA-2) was investigated in symptomless subjects with normal liver function tests to obtain data for their counseling and clinical management. Serum and hepatic HCV RNA were determined by the nested polymerase chain reaction, and liver histology was evaluated in 40 symptomless blood donors with stable indeterminate RIBA-2 pattern, including 38 reactive to c22-3. All but one had normal alanine aminotransferase (ALT) levels. Two new immunoblot tests, RIBA-3 and INNO-LIA HCV Ab III (LIA-III), which incorporate additional HCV antigens, were also done to assess whether they could identify the viremic subjects. Ten cases (25%, confidence interval 12 to 38) were HCV RNA positive. Three of the HCV RNA-positive and none of the HCV RNA-negative subjects had chronic hepatitis. RIBA-2 strong intensity of reaction (score > 2+) was observed in all the HCV RNA-positive and in 12 HCV RNA-negative subjects. RIBA-3 and LIA-III gave positive results in 9 of 10 and 10 of 10 HCV RNA-positive, but also in 8 of 30 and 24 of 30 HCV RNA-negative subjects. A c-22-3 reactivity score of 4+ by RIBA-3 and E2/NS1 reactivity by LIA-III were both strongly associated with HCV RNA (P < .001). Based on relatively high prevalence of chronic hepatitis in our series (30%), apparently healthy subjects with stable indeterminate RIBA-2 pattern and HCV RNA positivity should be considered for liver biopsy independently of ALT profile.(ABSTRACT TRUNCATED AT 250 WORDS)

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Prevalence of HCV-RNA-positive blood donors and correlation to ELISA and RIBA status

Cited by 20 publications

References 23 publications

HCV-lnfection in Blood Donors: Association between Anti-HCV Core IgM Antibodies and Serum HCV RNA

HCV-lnfection in Blood Donors: Association between Anti-HCV Core IgM Antibodies and Serum HCV RNA

Identification of Infection Routes for HCV in Blood Donors from Germany

Hepatitis C virus RNA and liver histology in blood donors reactive to a single antigen by second-generation recombinant immunoblot assay

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