Prevalence of Genotypes for Fimbriae and Enterotoxins and of O Serogroups in <i>Escherichia Coli</i> Isolated from Diarrheic Piglets in Korea

Kwon, Donghyok; Kim, Okjin; Chae, Chanhee

doi:10.1177/104063879901100207

Cited by 41 publications

(32 citation statements)

References 28 publications

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“…These data are consistent with those previously reported by Wilson & Francis (1986) and Kwon et al (1999). However, we also found this adhesin in weaned piglets with diarrhoea (Table 3).…”

Section: Haemolytic Activity In Strains Of E Colisupporting

confidence: 83%

Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico

Toledo

Gómez

Cruz

et al. 2012

Journal of Medical Microbiology

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Faecal Escherichia coli isolates from suckling (n5503) and weaning (n5450) piglets with and without diarrhoea from 10 farms in Mexico were examined for identification and prevalence of virulence genes. E. coli isolates were tested further for enterotoxin (LT, STa, STb, Stx1, Stx2 and EAST1), fimbrial (F4, F5, F6, F17, F18 and F41) and eae adhesin genes by multiplex PCR. Of the 953 isolates of E. coli examined by multiplex PCR, 650 (68.2 %) isolates were positive for at least one adhesin gene. Among the isolates from diarrhoeic piglets, F41 (72 %) was the most prevalent adhesin followed by eae adhesin (27 %), F6 (12 %), F17 (9 %), F18 (9 %), F5 (8 %) and F4 (3 %). Enterotoxin genes were detected in 424 (44.5 %) of the isolates, of which EAST1 (38 %) and STa (30 %) were the most common, followed by STb (17 %), Stx2 (6 %) and LT (5 %). Twenty-three per cent of isolates from suckling piglets and 43 % of isolates from weaned piglets carried both enterotoxin and adhesin genes, the most common virotypes being F41 : STa, F41 : EAST1, EAST1 : eae, F41 : F6, F41 : STa : STb, F41 : eae and F17 : eae. The present study examined for the first time, to our knowledge, the prevalence of 13 virulence genes among E. coli strains isolated from piglets with and without diarrhoea in Mexico. The results suggested that there are a wide variety of virulence genes associated with diarrhoea in piglets. This study provides baseline information on the significance of specific virotypes associated with suckling and weaning periods in piglets in Mexico.

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“…These data are consistent with those previously reported by Wilson & Francis (1986) and Kwon et al (1999). However, we also found this adhesin in weaned piglets with diarrhoea (Table 3).…”

Section: Haemolytic Activity In Strains Of E Colisupporting

confidence: 83%

Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico

Toledo

Gómez

Cruz

et al. 2012

Journal of Medical Microbiology

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“…The PCR for fimbriae and enterotoxins was carried out as previously described with slight modification. 4,7,13 Bacterial DNA amplification was performed using 5 l of the supernatant of lysed bacteria, 90 ng of oligonucleotide primers, 0.2 mM each of dATP, dGTP, dCTP, and dTTP, 10 mM Tris HCl (pH 8.8), 1.5 mM MgCl 2 , 50 mM KCl, and 1 unit of Taq polymerase, a all in 50 l of distilled water. The PCR profile used included a denaturing step at 94 C for 1 min, followed by annealing of the primers at T1 for 2 min, with an extension step at 72 C for 1 min.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of the Enteroaggregative Escherichia Coli Heat-Stable Enterotoxin 1 Gene and Its Relationship with Fimbrial and Enterotoxin Genes in E. Coli Isolated from Diarrheic Piglets

2001

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Abstract.A total of 720 Escherichia coli strains isolated from diarrheic piglets on 756 swine farms were screened for the presence of the enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1) gene by polymerase chain reaction (PCR). Escherichia coli strains that carried EAST1 genes were also tested by PCR for the presence of 4 fimbriae (F4, F5, F6, F41), 2 heat-stable enterotoxins (STa and STb), and 1 heat-labile enterotoxin (LT) gene. One hundred sixty-four (22.7%) of the 720 E. coli isolates carried genes for EAST1. Of these 164 isolates, 62 (37.8%) carried EAST1 genes only, 11 (6.7%) carried genes for at least 1 of the fimbrial adhesins, 51 (31.1%) carried genes for at least 1 of the enterotoxins, and 40 (23.8%) carried genes for at least 1 of the fimbrial adhesins and enterotoxins. Forty-six percent of strains that carried EAST1 genes carried STa genes, and 16% of strains that carried EAST1 genes carried F4. The isolation rate of enterotoxigenic E. coli strains carrying genes for EAST1 gene was 63%. The 6 major genotypes observed in this study (in decreasing order) were EAST1ϩ, EAST1ϩSTaϩ, EAST1ϩSTaϩSTbϩ, EAST1ϩSTaϩF5ϩ, EAST1ϩSTaϩF4ϩ, and EAST1ϩSTbϩF4ϩ. EAST1 is widely prevalent among diarrheagenic strains of E. coli and may represent an important virulence determinant in the pathogenesis of enteric colibacillosis of preweaned pigs.Escherichia coli is a major cause of diarrhea in human beings and animals. The 4 major groups are enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC), and enterohemorrhagic E. coli (EHEC). 5 Recently, evidence has accumulated to implicate a fifth distinct diarrheagenic category, enteroaggregative E. coli (EAggEC). The term enteroaggregative is derived from the pattern of adherence of bacteria to HEp-2 cells in culture. 6 EAggEC strains are a major cause of persistent diarrhea in children in the developing world. The aggregative adherence phenotype is associated with a bundle-forming pilus referred to as AAF/1. The genes for AAF/1 and a heat-stable enterotoxin 1 (EAST1) are carried on a 60-MD plasmid. EAST1 is genetically and immunologically distinct from heat-stable enterotoxin a (STa) produced by ETEC strains. 14,15 In addition to EAggEC, the EAST1 gene is also carried by human, porcine, and bovine ETEC strains. 1,10,15 The EAST1 gene sequence of F4ϩ ETEC strains is slightly different from that of human-colonizing ETEC strains. [14][15][16] The gene encoding EAST1 is highly prevalent among other groups of diarrheagenic E. coli isolated from humans, e.g., EHEC, ETEC, and EAggEC. 10 Although enteric colibacillosis is common in piglets, little is known concerning the prevalence of EAST1-positive E. coli and, thus, its potential significance in isolates from this species. The purpose of this study was to investigate the prevalence of the EAST1 gene in E. coli isolated from diarrheic piglets and to determine its relationship with other fimbrial (F4, F5, F6, and F41) and enterotoxin (STa, STb, and LT) genes by using polymerase chain reac...

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“…The genotypic analysis of the 285 isolates of E. coli tested in this study has been published elsewhere [2][3][4][8][9][10]. Among 199 isolates, 177 were ETEC and 22 were AEEC.…”

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confidence: 99%

Antimicrobial Susceptibility of Pathogenic Escherichia coli Isolated from Pigs in Korea.

Choi

Ham

Kwon

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. The in vitro susceptibilities of 285 isolates of Escherichia coli from preweaned and postweaned pigs with diarrhea and edema disease were tested with the 15 commonly used antimicrobial drugs by an agar dilution minimal inhibitory concentration procedure according to National Committee for Clinical Laboratory Standards (NCCLS) guidelines. All E. coli isolates tested in this study belonged to enterotoxigenic E. coli, attaching and effacing E. coli, or Shiga toxin-producing E. coli. Field isolates had low MIC 90 for ceftiofur (1 µg/ml). No correlation in antimicrobial resistance was found in three types of E. coli. Escherichia coli strains such as enterotoxigenic E. coli (ETEC), attaching and effacing E. coli (AEEC), and Shiga toxin-producing E. coli (STEC) are associated with diarrhea and edema disease in pigs. ETEC has 2 types of virulence factors, fimbrial adhesins and enterotoxins [5,14]. The production of attaching and effacing lesions by AEEC strains is associated with a chromosomal determinant, eaeA [7]. Shiga toxin 2e (Stx2e) produced by STEC plays an important role in the pathogenesis of edema disease in postweaned pigs [6,12]. AEEC have been identified as a cause of diarrhea in pigs [7]. The characteristic intestinal lesion resulting from AEEC is the attaching and effacing (A/E) histopathology, characterized by intimate bacterial adherence to enterocytes and disruption of the underlying cytoskeleton [15].Antimicrobial resistance is one of the main concerns for health professionals dealing with bacterial disease. The current trend worldwide is to reduce antibiotic use in animal production in favor of good husbandry practices and vaccination, as means to raise healthier animals. Acknowledging the susceptibility profiles of a given bacterium can help to select appropriate treatment for the infections caused by it, which reduces failure rates, and saves time and money. Isolation of such a pathogenic E. coli may be necessary for efficient treatment since numerous non-pathogenic E. coli mainly inhabit the intestinal tracts of pigs. This is a key point since mis-isolation of the causal strain could lead to treatment failure and an increase in antimicrobial resistance, but most of antimicrobial susceptibility studies did not focus on pathogenic E. coli prior to antimicrobial susceptibility procedures. The objective of this study was to select the pathogenic E. coli among E. coli isolated from pigs with diarrhea and edema disease in Korea, and determine the antimicrobial susceptibility of these pathogenic isolates by means of new standardized procedures for the antimicrobial susceptibility test for animal pathogens issued by the National Committee for Clinical Laboratory Standards (NCCLS) [16].Between 1995 and 1998, 853 isolates of E. coli were isolated from 957 diarrheic piglets younger than 14 days; and 250 isolates of E. coli were isolated from 294 postweaned pigs with diarrhea and edema disease submitted from all over Korea to the Department of Veterinary Pathology, Seoul National University. Specime...

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Prevalence of Genotypes for Fimbriae and Enterotoxins and of O Serogroups in Escherichia Coli Isolated from Diarrheic Piglets in Korea

Cited by 41 publications

References 28 publications

Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico

Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico

Prevalence of the Enteroaggregative Escherichia Coli Heat-Stable Enterotoxin 1 Gene and Its Relationship with Fimbrial and Enterotoxin Genes in E. Coli Isolated from Diarrheic Piglets

Antimicrobial Susceptibility of Pathogenic Escherichia coli Isolated from Pigs in Korea.

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