Prevalence of G class antibodies to antigens of lyme disease causes in dogs in Vojvodina, Serbia

Abstract

Lyme disease is a multisystemic disease, zoonotic in nature, caused by the Borrelia burgdorferi sensu lato complex. In the continent of Europe, these spirochetes are predominantly transmitted by ticks of the genus Ixodes. Small mammals and birds have particular significance as reservoirs of the cause of lyme disease. The objective of these epidemiological investigations was to determine the value of IgG seroprevalence to Borrelia burgdorferi and to secure the geographic distribution of seropositive dogs in Vojvodina. The investigations covered 135 dogs that were not vaccinated against lyme disease. The indirect ELISA test was used to determine IgG prevalence to Borrelia burgdorferi antigens. Reactive blood serums of dogs were tested again using the rapid immunochromatographic and immunoblot test. A seroprevalence of G class antibodies to antigens of lyme disease causes of 8.1% (11/135) was established in the examined dog population of Vojvodina. The biggest number of positive results was recorded for the South Bačka District. The presented value for the seroprevalence of anti-Borrelia burgdorferi antibodies in the dog population indicates the exhistence of a significant risk of humans becoming infected with the cause of lyme disease in Vojvodina

Tick-borne infections of dogs in Serbia: A review of research

“…IgG antibodies by ELISA (recomWell Borrelia canis IgG, Mikrogen, Germany) and Western blot assay (recomBlot Borrelia Canis IgG, Mikrogen, Germany). In that study, most of the positive findings were in South Bačka district area (Potkonjak et al, 2013b). In accordance with Potkonjak et al (2013b), Kovačević Berić et al reported lower average seroprevalence (0.07%) in dogs from North Bačka district (Berić .…”

Wild canids as hosts for ticks and tick-borne zoonotic pathogens in Serbia

“…In Europe, I. ricinus is the main vector, but the roles of many vertebrates as potential reservoir hosts are still unknown (Franke et al, 2013). Previous seroepidemiological studies in Serbia confirmed that domestic dogs are exposed to B. burgdorferi s.l (Obrenović et al, 2015;Potkonjak et al, 2013), while a high diversity of Borrelia species (B. burgdorferi s.s., Borrelia afzelii, B. lusitaniae, B. garinii, Borrelia bavariensis, and Borrelia valaisiana) were confirmed in I. ricinus ticks collected from vegetation (Ćakić et al, 2019;Tomanović et al, 2008).…”

Ixodid ticks and zoonotic tick-borne pathogens of the Western Balkans

“… I. ricinus Ticks 2.8 19.35 PCR-Seq PCR [ 165 ] [ 166 ] A. phagocytophilum I. ricinus , D. reticulatus , Hae. concinna , dog, golden jackal 0.9–15.5 IFAT, PCR-Seq, mf qPCR [ 158 , 161 , 163 , 165 , 166 , 174 , 176 , 178 , 179 ] N. mikurensis I. ricinus 4.2 PCR-Seq [ 12 , 165 , 166 ] E. canis Dog Case report, 16% IFAT, PCR-Seq [ 152 , 180 ] R. monacensis I. ricinus 14.8–22.5 PCR-Seq, mf qPCR [ 165 , 166 , 172 – 175 ] R. helvetica I. ricinus 7.4–54.0 PCR-Seq, mf qPCR [ 172 – 175 ] R. raoultii D. reticulatus 5.6 PCR-Seq, mf qPCR [ 165 , 172 ] …”

“…concinna , Rh. Sanguineus 3.8–62.9 PCR-Seq [ 174 , 183 ] B. burgdorferi s.l I. ricinus , dog, rodent 8.1–54.2 DF, IFAT, ELISA, WB, PCR [ 146 , 147 , 150 – 152 , 154 – 156 , 174 , 176 ] B. afzelii I. ricinus 7.0–75.0 Isolation, PCR-Seq, PCR/RFLP, RT-PCR [ 151 , 152 , 158 – 160 , 178 ] B. garinii I. ricinus , D. reticulatus , red fox 0.8–4.9 Isolation, PCR-Seq, PCR/RPLF [ 12 , 152 , 158 – 162 , 178 ] B. burgdorferi s.s I. ricinus , red fox 0.8–22.2 PCR-Seq, PCR/RFLP [ 12 , 152 , 159 , 178 ] B. valaisiana I. ricinus 1.4–3.8 Isolation, PCR-Seq, PCR/RFLP, mf qPCR [ 158 , 160 – 163 , 178 ] B. lusitaniae …”