Prevalence of extended-spectrum- -lactamase-producing Escherichia coli from pigs at slaughter in the UK in 2013

Randall, Luke; Lemma, Fabrizio; Rogers, Jon; Cheney, Tanya; Powell, L. F.; Teale, Christopher

doi:10.1093/jac/dku258

Cited by 41 publications

(27 citation statements)

References 22 publications

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“…For samples from the older pigs (samplings 2 and 3), faecal and slurry samples were first enriched (Randall et al . ) in buffered peptone water (BPW) for 18–24 h at 37°C, before plating onto MacConkey agar (McC) and MacConkey agar + 2 mg l −1 colistin (McC‐Col2) and incubating for 18–24 h at 37°C for isolation of E. coli. Salmonella were isolated by enrichment in BPW for 16–20 h at 37°C, then inoculated into modified semi‐solid Rappaport Vassiliadis (MSRV), as previously described (Anonymous, ), before plating onto other selective agars (Table , sampling 2 only).…”

Section: Methodsmentioning

confidence: 99%

Longitudinal study on the occurrence in pigs of colistin‐resistant Escherichia coli carrying mcr‐1 following the cessation of use of colistin

et al. 2018

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Section: Methodsmentioning

confidence: 99%

Longitudinal study on the occurrence in pigs of colistin‐resistant Escherichia coli carrying mcr‐1 following the cessation of use of colistin

et al. 2018

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“…Most ESBL derive from the first enzymes discovered, TEM (named after the patient (Temoneira) providing the first sample) and SHV (sulfhydryl reagent variable), which confer resistance to beta-lactams such as ampicillin or amoxicillin. The most recent ESBLs are derived from the CTX-M (active on cefotaxime-Munich) enzymes, which confer resistance to third-and fourth-generation cephalosporins [10,11]. Cephalosporins constitute one of the largest families of antimicrobials widely used in both human and veterinary medicine [12].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms

Vidal

Aguirre

Seminati

et al. 2020

Veterinary Sciences

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Escherichia coli is considered one of the most common agents associated with neonatal diarrhea in piglets. The aim of this work was to characterize the pathogenic and antimicrobial resistance (AMR) profiles of 122 E. coli strains isolated from pigs suffering diarrhea (n = 94) and pigs without diarrhea (n = 28) of 24 farms in Spain. Virulence factors, toxins and AMR (ESBL and colistin) genes and AMR phenotypes of E. coli isolates were analyzed. Low prevalence of pathogenic E. coli strains (26%) was found in both groups. However, ETEC and VTEC strains were more frequently isolated from diarrheic piglets. Irrespectively of diarrhea occurrence, 97.5% of the strains showed a multidrug-resistance (MDR) profile to aminopenicillins, sulfonamides and tetracyclines. It was found that 22% of E. coli was CTX-M+, with CTX-M-14 being the principal allelic variant. Remarkably, 81.5% of CTX-M+ strains were isolated from diarrheic animals and presented an extended MDR profile to aminopenicillins, quinolones and aminoglycosides. Finally, low frequencies of colistin resistance genes mcr-1 (4/122) and mcr-4 (1/122) were found. MDR E. coli strains are circulating in pig farms of Spain, representing a serious threat to animal and public health. More appropriate diagnostic approaches (genetic and AMR phenotypic analysis) should be implemented in animal health to optimize antibiotic treatments.

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“…One of the most important antimicrobial agents used for the treatment of serious infections in human and veterinary medicine are extended-spectrum cephalosporins, especially from third and fourth generations. In livestock, third-generation cephalosporins are used for respiratory and systemic infections caused by Streptococcus suis, including arthritis or mastitis in suckling and weaned piglets (Randall et al 2014). The fourth-generation cephalosporin cefquinome is licenced for the therapy of bacterial septicaemia in piglets and mastitis-metritis-agalactia syndrome in sows (Burch 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The occurrence of antimicrobial-resistant commensal bacteria has been studied extensively in food-producing animals worldwide (Dolejska et al 2011a;Dolejska et al 2011b;Agersø and Aarestrup 2012;Wu et al 2012;Randall et al 2014;Andersen et al 2015;Lucas et al 2018). Increasing occurence of ESBL-producing E. coli in livestock is of special concern for public health due to the potential risk of transfer of antimicrobial resistance determinants to human microbiome via the food chain, including pathogenic bacteria causing severe infections (Hansen et al 2013;Lucas et al 2018).…”

Section: Introductionmentioning

confidence: 99%

CTX‐M‐producing Escherichia coli in pigs from a Czech farm during production cycle

Zelendova

Dolejská

Masaříková

et al. 2020

Lett Appl Microbiol

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We evaluated the prevalence and epidemiology of extended‐spectrum beta‐lactamase (ESBL)‐producing Escherichia coli isolates in pigs during production cycle on a Czech farm with the history of previous use of ceftiofur. ESBL‐producing E. coli isolates were obtained from rectal swabs from pigs of different age groups (suckling piglets, weaned piglets, growers and sows). Collected samples were directly cultivated on MacConkey agar with cefotaxime (2 mg l−1), whereas intestinal swabs of slaughtered pigs and surface swabs from pig carcasses were also pre‐enriched in buffered peptone water without antimicrobials before the cultivation. Clonal relationship of selected isolates was determined by XbaI pulse‐field gel electrophoresis and multi‐locus sequence typing. The transferability of plasmids carrying blaCTX‐M genes was tested by conjugation experiments. From all examined samples, 141 (43·7%, n = 323) were positive for ESBL‐producing E. coli. All ESBL‐producing isolates showed resistance to multiple antimicrobials and were positive for blaCTX‐M genes. The blaCTX‐M‐1 was carried by conjugative IncN/ST1 plasmids (c. 40–45 kb) while the blaCTX‐M‐15 was located on conjugative F plasmids with F:18:A5:B1 formula (c. 165 kb). This study demonstrated the persistence of CTX‐M‐positive E. coli isolates 2 months after banner of ceftiofur usage and indicated possible risk of transmission of these isolates to humans via the food chain.

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Prevalence of extended-spectrum- -lactamase-producing Escherichia coli from pigs at slaughter in the UK in 2013

Abstract: In this UK study, 23.4% of pigs were found to be positive for ESBL-producing E. coli using selective culture media. The use of two different commercially available ESBL isolation media was found to improve the detection of ESBL-producing E. coli.

Cited by 41 publications

References 22 publications

Longitudinal study on the occurrence in pigs of colistin‐resistant Escherichia coli carrying mcr‐1 following the cessation of use of colistin

Longitudinal study on the occurrence in pigs of colistin‐resistant Escherichia coli carrying mcr‐1 following the cessation of use of colistin

Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms

CTX‐M‐producing Escherichia coli in pigs from a Czech farm during production cycle

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