Prevalence of extended-spectrum cephalosporinase (ESC)-producing Escherichia coli in Danish slaughter pigs and retail meat identified by selective enrichment and association with cephalosporin usage

Agersø, Yvonne; Aarestrup, Frank Møller; Pedersen, Karl; Seyfarth, Anne Mette; Struve, Tina; Hasman, Henrik

doi:10.1093/jac/dkr507

Cited by 93 publications

(76 citation statements)

References 25 publications

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“…The use of broad-spectrum veterinary cephalosporins (especially "third-generation" and "fourth-generation" cephalosporins, such as ceftiofur and cefquinome) has been proposed as an important reason for the occurrence of these bacteria among food-producing animals and meat products (6). In Denmark, the consumption of cephalosporins in pigs increased from 2001 through July 2010, when the pig industry decided to ban their use (26).…”

Section: Discussionmentioning

confidence: 99%

“…CTX-M-1 is the most frequently reported ESBL type in food animals in Europe, and this was also the first type discovered in Danish pigs in 2005 (5). The same type was also predominant in a recent study of pigs at slaughter in Denmark, followed by CTX-M-14, CTX-M-2, and CTX-M-15 (6). In Europe, CTX-M-1 and CTX-M-14 have also been detected among pigs in Spain, France, the United Kingdom, and Switzerland (7)(8)(9)(10)(11)).…”

mentioning

confidence: 86%

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Carriage and Fecal Counts of Cefotaxime M-Producing Escherichia coli in Pigs: a Longitudinal Study

Hansen

Damborg

Andreasen

et al. 2013

Appl Environ Microbiol

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c Current knowledge on extended-spectrum beta-lactamases (ESBLs) in animals is based largely on cross-sectional studies and qualitative data. The aim of this longitudinal study was to elucidate carriage proportions and fecal counts of ESBL-producing Escherichia coli in pigs during the production cycle. At each of three ESBL-positive single-sited farrow-to-finisher pig farms (farms A, B, and C) included in the study, individual fecal samples were taken from 17 to 20 sows 1 week before farrowing and from 2 piglets of each sow's litter four times from birth to slaughter (as piglets, weaners, and finishers). Cefotaxime (CTX)-resistant coliforms in feces were counted on MacConkey agar containing 2 g/ml CTX and characterized for the presence of ESBLencoding genes by PCR and sequencing. CTX-M-positive pigs were detected in all age groups at farms A (bla CTX-M-9 group, compatible with bla CTX-M-14/17 ) and B (bla CTX-M-1 group, compatible with bla CTX-M-1/61 ), whereas only three weaners were positive at farm C (bla CTX-M-1 group, compatible with bla CTX-M-1/61 ). A significant decrease in carriage was detected during the production cycle, with on average 50% carriage immediately after birth, 58% just before weaning, 29% during weaning, and 12% during finishing. The observed reduction in numbers of CTX-M-positive pigs was accompanied by a significant reduction in mean fecal counts of CTX-resistant coliforms from ϳ10 7 CFU/g in piglets to ϳ10 3 CFU/g in finishers (P < 0.001). These findings provide novel information about the epidemiology of ESBLs at the farm level and have important implications for assessments of risks of meat contamination during slaughter.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 86%

Carriage and Fecal Counts of Cefotaxime M-Producing Escherichia coli in Pigs: a Longitudinal Study

Hansen

Damborg

Andreasen

et al. 2013

Appl Environ Microbiol

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“…In addition, the results also showed that the overall prevalence of ESBL-E. coli is higher in Thai Binh than in Nha Trang. A study conducted in Denmark reported that 36 % of samples from imported broiler chicken were contaminated with extendedspectrum cephalosporinase-producing E. coli (Agersø et al 2012), while a similar study conducted in the Netherlands showed that 76.8-94 % of poultry products were contaminated with ESBL-E. coli (Leverstein-van Hall et al 2011; Overdevest et al 2011). Thus, the prevalence of ESBLproducing bacteria in food products varies in different geographical areas.…”

Section: Discussionmentioning

confidence: 99%

Widespread dissemination of extended-spectrum β-lactamase-producing, multidrug-resistant Escherichia coli in livestock and fishery products in Vietnam

Kawahara

Khong

et al. 2015

FoodContamination

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Background: Widespread dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria is a threat to public health. Since livestock products are possible reservoirs of ESBL-producing bacteria, food-borne dissemination of ESBL-producing bacteria and the characteristics of such organisms in food products should be assessed to evaluate potential sources of contamination. Results: A total of 253 food samples from poultry, pork, shrimp, and fish were collected from local markets in a rural area of Vietnam from 2013 to 2014. ESBL-producing Escherichia coli were isolated from these samples, and their ESBL genotypes, phylogenetic groups, and antibiotic resistance profiles were assessed. Overall, a high percentage (68.4 %) of the food samples was contaminated with ESBL-producing E. coli, and samples from livestock and fishery products showed similar levels of contamination. The ESBL-producing E. coli isolated from the food samples harbored genes of the CTX-M-1, CTX-M-9, and TEM groups. Analysis of the antibiotic resistance profiles of the ESBL-producing E. coli isolates from the food samples showed a high degree of multidrug resistance. The prevalence of extensively multidrug-resistant ESBL-producing E. coli that were resistant to at least five antibiotic drug classes in poultry, pork, shrimp, and fish samples, was 92.1, 69, 56.5, and 62.5 %, respectively. Conclusions: The results of this study confirmed the widespread dissemination of ESBL-producing E. coli in both livestock and fishery products from a rural area in Vietnam. The high prevalence of extensively multidrug-resistant ESBL-producing E. coli in food products highlights the importance of continuous monitoring of food products for the presence of these bacteria, particularly in underdeveloped countries.

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“…Similarly, in Asia and Europe, ESC resistance in E. coli isolated from livestock has been attributed to the emergence and spread of plasmidmediated bla CTX-M genes and bla CMY-2 [6][7][8]. In several countries in these regions, extensive use of FQs in some food-animal species has been linked to the emergence of FQ-resistant E. coli and Salmonella [9,10].…”

Section: Plasmid-mediated Esc Resistance (Mediated By Bla Cmy-2 ) Wasmentioning

confidence: 99%

First detection of extended-spectrum cephalosporin- and fluoroquinolone-resistant Escherichia coli in Australian food-producing animals

Abraham

Jordan

Wong

et al. 2015

Journal of Global Antimicrobial Resistance

100

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ampicillin (9.4-71.1%), trimethoprim/sulfamethoxazole (11.1-67.5%) and streptomycin (21.9-69.3%), whereas none were resistant to imipenem or amikacin.Resistance was detected, albeit at low frequency, to ESCs (bovine isolates, 1%; porcine isolates, 3%) and FQs (porcine isolates, 1%). Most ESC-and FQ-resistant isolates represented globally disseminated E. coli lineages (ST117, ST744, ST10 and ST1). Only a single porcine E. coli isolate (ST100) was identified as a classic porcine enterotoxigenic E. coli strain (non-zoonotic animal pathogen) that exhibited ESC resistance via acquisition of bla CMY-2 . This study uniquely establishes the presence of resistance to CIAs among clinical E. coli isolates from Australian foodproducing animals, largely attributed to globally disseminated FQ-and ESC-resistant E. coli lineages.

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Prevalence of extended-spectrum cephalosporinase (ESC)-producing Escherichia coli in Danish slaughter pigs and retail meat identified by selective enrichment and association with cephalosporin usage

Abstract: The usage of cephalosporins for slaughter pigs may increase the prevalence of ESC E. coli in slaughter pigs. Meat may be a source of ESCs in humans, especially imported broiler meat. Selective enrichment should be considered as a supplementary surveillance method.

Cited by 93 publications

References 25 publications

Carriage and Fecal Counts of Cefotaxime M-Producing Escherichia coli in Pigs: a Longitudinal Study

Carriage and Fecal Counts of Cefotaxime M-Producing Escherichia coli in Pigs: a Longitudinal Study

Widespread dissemination of extended-spectrum β-lactamase-producing, multidrug-resistant Escherichia coli in livestock and fishery products in Vietnam

First detection of extended-spectrum cephalosporin- and fluoroquinolone-resistant Escherichia coli in Australian food-producing animals

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