Prevalence of Escherichia coli Pathotypes Among Children With Diarrhea in Babol, Northern Iran

Abstract

Background: Diarrheagenic Escherichia coli (DEC) are major causes of diarrhea in the world particularly among infants and young children. Objectives: The aim of this study was to determine the prevalence of DEC strains in stool samples from children under 5 years old. Patients and Methods: Stool specimens were collected from 200 children under 5 years visiting hospital due to gastroenteritis. E. coli pathotypes were detected by using conventional culture techniques and polymerase chain reaction (PCR). Results: Sixty-eight (34%) out of 200 specimens were positive for DEC. Different pathotypes would show the following profiles: 43 (21.5%) for enteropathogenic E. coli (EPEC); 18 (9%) for enterotoxigenic E. coli (ETEC) including 10 (55.5%) st positive, 6 (33.3%) lt positive and 2 (11.1%) st and lt both positive; 6 (3%) for enteroaggregative E. coli (EAEC) and 1 (0.5%) for enteroinvasive E. coli (EIEC). Enterohemorrhagic E. coli (EHEC) was not isolated from any of the E. coli strains tested. Conclusions: This study shows that DEC is a common cause of childhood diarrhea in Babol. EPEC and ETEC were the most frequent pathotypes in the population under study.

Molecular Characterization and Antimicrobial Resistance of Enteropathogenic Escherichia coli in Children from Ahvaz, Iran

“…Moreover, severe malabsorption of nutrients might be caused by EPEC, helping the nutritional aggravation and the persistence of diarrhea (5). In the present study, the results of PCR detection of eaeA gene were indicative of 6.78% frequency of EPEC in the E. coli isolates, which was higher, compared to the prevalence rate mentioned in two reports by Nakhjavani (5.6%), Asadi Karam (5.3%) and lower than report by Moshtagian (21.5%) in Iran (30)(31)(32), and also higher from some neighboring countries, including Iraq (3.4%) and Turkey (2.05%) (33,34). This rate was also lower, compared to the reports from Kuwait (8.4%) and Pakistan (35,36).…”

Diarrhoea and Associated Clinical Features in Different Pathotypes of Diarrheagenic E. coliIsolated in Children: A Case-Control Study in a Tertiary Care Hospital

“…who reported ETEC as the most common pathotype. 38,42 The result of the present study differs from the studies done by Hegde A et al Moshtagian F, et al, Dutta S, et al who reported that EAEC (50%), EPEC (63.2%) and EAEC (48.7%) respectively as the commonest pathotype. 36,37,43 In this study, we did not detect any EAEC, EIEC or DAEC strain from cases.…”

<p>Distribution of Genes Encoding Virulence Factors and the Genetic Diversity of Enteroinvasive <em>Escherichia coli</em> (EIEC) Isolates from Patients with Diarrhea in Ahvaz, Iran</p>

“…This was in agreement with the findings in the previous study from Shiraz (5.5%). 17 Compared with our findings, lower prevalence rates of EIEC have been reported from Northern Iran (0.5%), 18 Nigeria and Vietnam (0.8%). 19,20 The high frequencies of the ipaH gene could explain frequencies of fever, vomiting, and dehydration in infected children.…”

“…Of 13 strains of EIEC, 84.6% (n=11) isolated from children under the age of 2 years (P<0.05). Children were categorized into five different groups according to their age: (0-12), (13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24), (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36), (35-48), and (49-60) months. Distribution of EIEC strains according to age, gender, and clinical symptoms are shown in Table 3.…”