Prevalence of Escherichia coli O157:H7 among Diarrhoeic HIV/AIDS 
 Patients in the Eastern Cape Province-South Africa

Abong'o, B. O.; Momba, Maggy Ndombo Benteke; Malakate, V K; Mwambakana, Jeanine

doi:10.3923/pjbs.2008.1066.1075

Cited by 15 publications

(7 citation statements)

References 21 publications

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“…lower prevalence rates of E. coli O157 in milk and dairy products (2% and 4%) in Egypt than that we detected in this study(El-Atty & Meshref, 2008) Abong'o et al (2008). in South Africa found E. coli O157 in 10.3% (4 out of 39) of vegetable samples, which was similar to the contamination rate detected in this study(Abong'o et al, 2008).…”

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confidence: 85%

A quantitative prevalence of Escherichia coliO157 in different food samples using real‐time qPCR method

Pakbin

Brück

et al. 2022

Food Science & Nutrition

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Escherichia coli serogroup O157 is the main causative agent of several intestinal and extra‐intestinal foodborne diseases in humans through consumption of low‐dose contaminated foods such as milk, beef, and vegetables. To date, studies regarding the quantitative prevalence of E. coli O157 in foods are so limited. Therefore, this study aimed to evaluate the quantitative prevalence rate of E. coli serogroup O157 in raw milk (n = 144), vegetable salad (n = 174), and minced beef samples (n = 108) using the real‐time qPCR SYBR green melting curve method targeting the rfbA gene. First, we evaluated the method and found a sensitive and specific qPCR assay with 1 log of CFU/ml detection limit to detect E. coli O157 (Tm = 80.3 ± 0.1°C). About 2.77%, 10.18%, and 9.19% of raw milk, minced beef, and vegetable salad samples, respectively, were contaminated with E. coli O157. Minced beef and vegetable salad samples were significantly more contaminated than raw milk samples. Population average of E. coli O157 in raw milk, minced beef, and vegetable salad samples were 2.22 ± 0.57, 3.30 ± 0.40, and 1.65 ± 0.44 log CFU/ml or gr, respectively. Significantly higher levels of population of E. coli O157 were observed in minced beef samples. Minced beef can be regarded as the main food in the transmission of this foodborne pathogen. Routine quantitative rapid monitoring is strongly suggested to be carried out to prevent foodborne diseases caused by E. coli O157.

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confidence: 85%

A quantitative prevalence of Escherichia coliO157 in different food samples using real‐time qPCR method

Pakbin

Brück

et al. 2022

Food Science & Nutrition

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“…It has been also demonstrated that this pathogen was involved in diarrhea in HIV pediatric patients [11, 12]. Escherichia coli remains the predominant Gram negative uropathogen isolated in acute community uncomplicated infections, followed by Klebsiella, Enterobacter and Proteus species [13].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility patterns of enterobacteriaceae isolated from HIV-infected patients in Kinshasa

Iyamba¹,

Wambale²,

Takaisi-Kikuni³

2014

Pan Afr Med J

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IntroductionPeople infected by Human Immunodeficiency Virus (HIV) are susceptible to develop severe bacterial infections. We set out to determine the frequency and the sensitivity to antibiotics of enterobaceriaceae isolated from urine and feces of HIV-infected persons.MethodsUrine and feces samples were collected from HIV-infected patients of the Centre de Traitement Ambulatoire de Kabinda (CTA/Kabinda, Kinshasa) and analyzed at the Reference National Laboratory for HIV/AIDS and Sexually Transmitted Infections. The isolated enterobacteriaceae strains were identified by conventional microbiological methods. Antibiotic sensitivity pattern was carried out by disc diffusion method.ResultsThe following bacteria pathogens were isolated: Escherichia coli, Klebsiella, Enterobacter, Proteus, and Providencia. Most species were sensitive to cefotaxim, ceftriaxon, and gentamicin and resistant to chloramphenicol, cotrimoxazole, tetracycline, and norfloxacin.ConclusionThe results of the present study show that the most frequently bacteria isolated were Esherichia coli and cefotaxim, ceftriaxon, and gentamicin were the most active antibiotics.

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“…31 Further studies reported a prevalence of 56.5% and 43.5% from stool of confirmed and non-confirmed HIV/AIDS patients, respectively, in the Eastern Cape province. 32 The STEC O157:H7 isolates from meat products (7.8%), water (8.6%), vegetables (10.3%), confirmed HIV/AIDS patients (56.5%) and non-confirmed HIV/ AIDS patients (43.5%) were genetically related; 30 and, hence, provided evidence on the possible transfer of the pathogens between different study components. In the neighbouring country of Botswana, the prevalence of STEC O157:H7 in meat cubes, minced meat and fresh sausages in Gaborone were reported to be 5.22, 3.76 and 2.26%, respectively.…”

Section: Reports Of Stec O157:h7 Occurrence In Africamentioning

confidence: 99%

“…56,57 Some studies have employed both sorbitol MacConkey agar and immunomagnetic separation to maximise the chances of isolating the pathogen. 32 STEC O157:H7 strains should be distinguished from Non-O157:H7 strains, which also do not ferment sorbitol. 18 Either of these E. coli isolation options can be accomplished by performing an agglutination test using antibodies against a somatic antigen for O157:H7 and a flagella antigen for H7.…”

Section: Isolation and Characterisation Of Stec O157:h7mentioning

confidence: 99%

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Epidemiology of Shiga toxin-producing Escherichia coli O157:H7 in Africa in review

Lupindu

2018

Southern African Journal of Infectious Diseases

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Shiga toxin-producing Escherichia coli (STEC) O157:H7 is responsible for intestinal and extra-intestinal disease syndromes in human. Isolation of the pathogen from animals, food, clinical samples and environment has been reported from all continents. A review of STEC O157:H7 in Africa from a structured literature search of the PubMed electronic database is presented. It describes the epidemiological status of the pathogen on the aspects of source, transmission, pathogenesis, disease syndromes, diagnosis, disease burden and the challenges in treatment and control strategies. About a quarter of African countries have reported isolation of STEC O157:H7 either from humans, animals, food or the environment. Different methods have been used in detection of the pathogen. Most reported human infections do not show temporal relationships with reports of isolation of the pathogen from other sources such as animals, water or food. Lack of a direct link between isolates from humans and other sources makes it difficult to point out incident specific determinants and direction of transmission. The aim of this review is to give an insight into the features of STEC O157:H7 infection in Africa and draw the attention of various stakeholders to the public health threat of the pathogen for possible interdisciplinary and multi-sectoral joint efforts in the control strategies.

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Prevalence of Escherichia coli O157:H7 among Diarrhoeic HIV/AIDS Patients in the Eastern Cape Province-South Africa

Cited by 15 publications

References 21 publications

A quantitative prevalence of Escherichia coliO157 in different food samples using real‐time qPCR method

A quantitative prevalence of Escherichia coliO157 in different food samples using real‐time qPCR method

Antimicrobial susceptibility patterns of enterobacteriaceae isolated from HIV-infected patients in Kinshasa

Epidemiology of Shiga toxin-producing Escherichia coli O157:H7 in Africa in review

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