Prevalence of Escherichia coli O157 from Cattle and Foods in the Mekong Delta, Vietnam

Khai, Lý Thị Liên; Phan, Tran Thi; Tam, Nguyen Thu; 剛敏, 岩田; 秀樹, 小林; 友三アレシャンドレ, 岡谷; 隆秀, 谷口; Toan, Ha Thanh; 秀樹, 林谷

doi:10.2743/jve.13.107

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…From each positive sample, ten suspicious E. coli colonies were selected for subculture on nutrient agar (NA, Merck, Germany). After incubation for 24 hours at 37°C, those isolates were individually examined for biochemical tests as previously described [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Prevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam

Nguyen

Thuan

Tam

et al. 2021

Veterinary Medicine International

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Avian pathogenic Escherichia coli (APEC) is the main causative agent of avian colibacillosis, which is an important systemic disease of profound economic and clinical consequences for the poultry industry worldwide. In this study, 975 E. coli strains were isolated from 2,169 samples collected from cloacal swabs of chickens, in-farm wild animals (ants, geckos, flies, and rats), and environment. The highest proportion of E. coli isolation was obtained from chicken cloacal swabs with 71.05% (95% confidence interval (CI) 66.69–75.05%) followed by the proportions of 38.15% (95% CI 35.41–40.97%) and 38.11% (95% CI 34.15–42.24%) from wild animals or environment, respectively. Distribution of O-antigen serotypes of the E. coli isolates, including O1, O2, O18, and O78, was determined by PCR. The most predominant serotype was O18 (10.56%) followed by O2 (9.44%), O1 (7.79%), and O78 (6.56%). Of note, serotype O18 was more likely distributed in the examined wild animals, especially in geckos. Polymorphic DNA fingerprints, generated by ERIC-PCR, of representative E. coli strains of each serotype revealed genetic heterogeneity of the examined E. coli, and O18 was more divergent with 63 clusters formed from 66 isolates. Furthermore, several E. coli strains from different sample sources shared high DNA fingerprint relatedness, suggesting that there exists complex transmission of E. coli from chickens to wild animals and environment and vice versa in poultry husbandry settings. Although pathotypes of the examined E. coli were not determined in this study, our results provided important findings of epidemiological and genetic characteristics of E. coli in the Mekong Delta and highlighted the prerequisite of stricter biocontainment to reduce the prevalence and consequences of APEC in poultry production.

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Section: Methodsmentioning

confidence: 99%

Prevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam

Nguyen

Thuan

Tam

et al. 2021

Veterinary Medicine International

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“…In most resistance patterns, ampicillin (Am) was present the most frequent in this study (Table 2). Previous reports in the Mekong Delta recorded that E. coli isolated from farm animals demonstrated high resistance to ampicillin, and this antibiotic was usually detected in the resistance patterns (Ly et al, 2009;Bui et al, 2018). In Nigeria, Olatoye (2010) indicated that the misuse of antibiotics in beef cattle could cause an increase of multiple resistance antibiotics of E. coli, especially EHEC O157:H7.…”

Section: Discussionmentioning

confidence: 97%

Prevalence of antibiotic resistance genes and genetic relationship of Escherichia coli serotype O45, O113, O121, and O157 isolated from cattle in the Mekong Delta, Vietnam

Nguyen

Nguyễn

Nguyen

et al. 2022

VIS

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A total of 39 Escherichia coli strains serotype O45, O113, O121, and O157 isolated from cattle in the Mekong Delta were examined the antimicrobial susceptibility to 13 antibiotics by the disc-diffusion method. Those strains were also analyzed for the presence of antibiotic resistance genes by PCR assay, and their genetic relationship by ERIC-PCR assay. The results of antimicrobial susceptibility testing showed that those strains were sensitive to most of the examined antibiotics, but were relatively high resistance to ampicillin (64.10%), and colistin (53.85%). Those E. coli strains could be resistant against one to eight antibiotics with 22 resistance patterns obtained. Moreover, those E. coli strains harbored one to seven antibiotic resistance genes. Gene tetA (51.28%) and blaampC (48.72%) were detected frequently while gene tetB, blaCMY, and cat1 were not found in those E. coli strains. A total of 21 combined patterns of antibiotic resistance genes were recorded, and the most frequent combined pattern was blaampC+tetA (12.82%). ERIC-PCR analysis revealed that each E. coli serotype exhibited various genetic patterns with 40%-100% of similarity. The most elevated number of patterns were in E. coli O157 (nine patterns), followed by E. coli O121 (six patterns). The prevalence of antibiotic resistance genes and diverse genetic characteristics in those E. coli strains originated from cattle constitute potential risks to local health in the Mekong Delta.

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Sự hiện diện của gene độc lực và tính đề kháng kháng sinh của vi khuẩn Escherichia coli O157:H7/H- phân lập từ bò tại Đồng bằng sông Cửu Long

Thuan¹,

Khai²

2020

CTU Journal

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Prevalence of Escherichia coli O157 from Cattle and Foods in the Mekong Delta, Vietnam

Cited by 5 publications

References 35 publications

Prevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam

Prevalence and Genetic Relationship of Predominant Escherichia coli Serotypes Isolated from Poultry, Wild Animals, and Environment in the Mekong Delta, Vietnam

Prevalence of antibiotic resistance genes and genetic relationship of Escherichia coli serotype O45, O113, O121, and O157 isolated from cattle in the Mekong Delta, Vietnam

Sự hiện diện của gene độc lực và tính đề kháng kháng sinh của vi khuẩn Escherichia coli O157:H7/H- phân lập từ bò tại Đồng bằng sông Cửu Long

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