Prevalence of Escherichia coli carrying extended-spectrum  -lactamases (CTX-M and TEM-52) from broiler chickens and turkeys in Great Britain between 2006 and 2009

Randall, Luke; Clouting, C.; Horton, Robert A.; Coldham, N. G.; Wu, Guanghui; Clifton-Hadley, F. A.; Davies, Rob; Teale, C. J.

doi:10.1093/jac/dkq396

Cited by 154 publications

(157 citation statements)

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“…Similar results have been reported by Randall et al [13] , Overdevest et al [3] , Morris et al [26] , and Leverstein-van Hall et al [27] . Also, the majority of those ESBL genes were identified as bla CTX-M-1 (81.8%) in this study.…”

Section: Discussionsupporting

confidence: 90%

“…The prevalence of ESBL producing E. coli has been reported between 6.7% and 60.7%. Our results were relatively low according to some studies [9,13] while showed similarities to others [11,12] .…”

Section: Discussionsupporting

confidence: 70%

“…The use of cephalosporins in food-producing animals and in veterinary medicine could be a selective factor for the appearance of ESBL producing bacteria in animals [7] . Recent studies in Spain [8] , France [9] , Tunisia [10] , Belgium [11] , China [12] , Great Britain [13] , Netherlands [6] , and England [14] show that CTX-M E. coli isolates are likely to be present in chickens globally. In Turkey, the prevalence has not been extensively investigated in poultry.…”

Section: Introductionmentioning

confidence: 99%

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Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz Üreten Escherichia coli İzolatlarının Saptanması

Kahraman¹,

Sığırcı²,

Çelik³

et al. 2016

Kafkas Univ Vet Fak Derg

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The aim of this study was to investigate the presence of Extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Escherichia coli strains isolated from meat and faecal samples of chicken. Faecal samples (n: 384) were collected from broiler and egg-type healthy chickens in 43 flocks and 384 fresh chicken meat samples from local markets, supermarkets and slaughterhouses. E. coli isolates were tested phenotypically according to the guidelines of CLSI (2013) for ESBL production. Phenotypic detection of AmpC production was carried out by determination of resistance to cefoxitin and susceptibility to cefepime. Also, presence of genes encoding different types of β-lactamases (blaCTX-M, blaTEM, blaSHV and the mutation in the promoter of AmpC) in all phenotypically ESBL or AmpC producing E. coli was investigated by PCR assays. In this study, blaCTX-M (95.4%), blaCTX-M-1 (81.8%), blaCTX-M-8 (4.5%), blaTEM (45.4%) and blaSHV (2.2%) genes were detected from faecal samples and blaCTX-M (100%), blaCTX-M-1 (28.5%) and blaTEM (7.1%) from meat samples. The only plasmidic AmpC β-lactamase found was the CIT type. Keywords: Escherichia coli, ESBL, AmpC, Chicken, PCR Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz üreten Escherichia coli İzolatlarının Saptanması ÖzetBu çalışmanın amacı, tavuk eti ve dışkı örneklerinden izole edilen Genişlemiş Spektrumlu β-laktamaz (GSBL) ve AmpC β-laktamaz üreten Escherichia coli izolatlarının varlığını ortaya koymaktır. Bu çalışmada, 43 kümesten sağlıklı tavuklara ait 384 dışkı örneği ve yerel marketlerden, süpermarketlerden ve kesimhanelerden 384 taze tavuk eti örneği toplandı. E. coli izolatları fenotipik olarak CLSI (2013) standartları kullanılarak GSBL üretimi yönünden incelendi. AmpC üretiminin fenotipik olarak saptanması sefoksitine direnç ve sefepime duyarlılık kriterleri ile belirlendi. Fenotipik olarak GSBL ya da AmpC üreten E. coli olarak saptanan izolatlarının hepsi PCR ile değişik tipteki β-laktamaz genlerinin (blaCTX-M, blaTEM, blaSHV, AmpC) varlığını ortaya koymak için incelendi. Bu çalışmada, dışkı örneklerinde blaCTX-M (%95.4), blaCTX-M-1 (%81.8), blaCTX-M-8 (%4.5), blaTEM (%45.4), blaSHV (%2.2) genleri ve tavuk eti örneklerinde ise blaCTX-M (%100), blaCTX-M-1 (%28.5), blaTEM (%7.1) genleri saptandı. AmpC β-laktamaz grubunda sadece CIT geni belirlendi.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

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Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz Üreten Escherichia coli İzolatlarının Saptanması

Kahraman¹,

Sığırcı²,

Çelik³

et al. 2016

Kafkas Univ Vet Fak Derg

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“…A major concern is the high prevalence of these resistant bacteria in the broiler production chain. ESBL‐/pAmpC‐producing Enterobacteriaceae were frequently isolated from broiler fattening farms worldwide (Bortolaia et al ., 2010; Randall et al ., 2011; Kameyama et al ., 2013; Maciuca et al ., 2015; Trongjit et al ., 2016) but were also found in broiler (grand) parent flocks (Dierikx et al ., 2013; Agersø et al ., 2014; Mo et al ., 2014; Zurfluh et al ., 2014). Therefore, different transmission scenarios of the resistant bacteria in the broiler production pyramid are discussed: A transmission from prior stages into the fattening farms (Giovanardi et al ., 2005; Dierikx et al ., 2013; Nilsson et al ., 2014; Huijbers et al ., 2016) as well as an insufficient cleaning and disinfection procedure in the chicken barns (Hiroi et al ., 2012; Luyckx et al ., 2015b).…”

Section: Introductionmentioning

confidence: 99%

Environmental adaptation and vertical dissemination of ESBL‐/pAmpC‐producing Escherichia coli in an integrated broiler production chain in the absence of an antibiotic treatment

Projahn

Daehre

Semmler

et al. 2018

Microbial Biotechnology

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SummaryHigh prevalence numbers of extended‐spectrum beta‐lactamase‐ (ESBL‐)/plasmid‐mediated AmpC beta‐lactamase‐ (pAmpC‐) producing Escherichia coli in broiler chicken and their distribution along the broiler production chain is an ongoing problem in food production. We, therefore, investigated resistant isolates along the broiler production chain to determine whether there is a constantly occurring direct vertical transmission of the ESBL‐/pAmpC‐producing E. coli from the parent flocks to their offspring or not. We, furthermore, analysed the isolates concerning the occurrence of virulence factors and their ability to form biofilms to estimate their potential to effectively colonize broiler chickens and/or persist and survive in the environment of the broiler production facilities. Using whole genome sequencing, we could show that ESBL‐/pAmpC‐producing E. coli were likely transferred in a step‐wise process along the broiler production chain but not directly from the parent flock to the fattening flock with every single batch of offspring chickens. Additionally, resistant E. coli strains showing an extraintestinal pathogenic genotype as well as high numbers of virulence‐associated genes including the production of curli fibres and cellulose have high capabilities to persist and spread in the broiler production chain.

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“…The gastro-intestinal tract of animals is a reservoir for bacteria carrying β-lactamases and a potential source of human pathogens. In particular, ESBL has been described in healthy poultry, in faecal samples of broilers and also in broiler chicken caecal samples and turkey flocks (Costa et al 2009;Bortoloaia et al 2011;Randall et al 2011). Recently, a high prevalence (100%) was found in faecal samples of broilers in Germany, while no ESBLs producing bacteria were observed in turkey farms (Friese et al 2013).…”

mentioning

confidence: 99%

ESBL-producing bacteria and MRSA isolated from poultry and turkey products imported from Italy

et al. 2015

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Beninati C., Reich F., Muscolino D., Giarratana F., Panebianco A., Klein G., Atanassova V. (2015): ESBL-producing bacteria and MRSA isolated from poultry and turkey products imported from Italy. Czech J. Food Sci., 33: 97-102.ESBL and MRSA-producing bacteria in food-producing animals may contribute to increased incidences of infection in humans. This study was carried out on 38 samples obtained from 32 chickens and 6 turkey products purchased at retail outlets of Hannover (Germany) and imported from Italy. The samples included the thigh, breast fillet, gizzard, sausage, liver, heart, and roll, and were processed for the detection of ESBL producing E. coli and MRSA producing Staphylococcus aureus. Twenty-six chicken products (68.4%) of the total of poultry products analysed proved to be positive for E. coli and for phenotypical detection of ESBL. Six turkey products (100%) were positive for ESBL producing E. coli. Staphylococcus aureus was found in 4 chicken products (10.52%) that resulted positive in the detection of MRSA. Serratia spp. were reported in 4 samples that were also positive for ESBL. Thirty-three E. coli isolates from the poultry and turkey products were all resistant to at least one or more of the compounds tested. The highest resistance levels were observed, notably, against ampicillin and cefotaxim.

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Prevalence of Escherichia coli carrying extended-spectrum -lactamases (CTX-M and TEM-52) from broiler chickens and turkeys in Great Britain between 2006 and 2009

Abstract: poultry-derived CTX-M E. coli in GB are different from major CTX-M sequence types causing disease in humans.

Cited by 154 publications

References 31 publications

Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz Üreten Escherichia coli İzolatlarının Saptanması

Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz Üreten Escherichia coli İzolatlarının Saptanması

Environmental adaptation and vertical dissemination of ESBL‐/pAmpC‐producing Escherichia coli in an integrated broiler production chain in the absence of an antibiotic treatment

ESBL-producing bacteria and MRSA isolated from poultry and turkey products imported from Italy

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