Prevalence of Erysipelothrix Species in Healthy, Slaughtered Cattle.

Hassanein, Raafat; Sawada, Takuo; Kataoka, Yuki; Ali, Mohamed Abdelaal; Itoh, K.; Suzuki, Yoshikazu

doi:10.2743/jve.4.71

Cited by 8 publications

(8 citation statements)

References 10 publications

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“…The same study also found a higher rate of seropositive cattle in areas with concurrent swine industry [3]. This data could indicate that Erysipelothrix is mainly transmitted by pigs although cattle may also act as a vehicle for its distribution [5,16]. In support of this, Erysipelothrix was isolated from cattle slurry [3] which could enhance the bacterium's ability to spread as Erysipelothrix can survive in soil contaminated with faecal material [4].…”

Section: Introductionmentioning

confidence: 71%

“…During studies in Japanese abattoirs, Erysipelothrix was isolated from 6.4% of 1236 healthy, slaughtered cattle [16] which demonstrates that cattle may be subclinically infected with the bacterium. An epidemiological follow-up study using the growth agglutination test (GAT) to detect antibodies anti-Erysipelothrix in Japanese cattle found that 76% of 854 healthy cattle had detectable antibodies [3].…”

Section: Introductionmentioning

confidence: 99%

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Use of the rSpaA415 antigen for detection of IgG anti-Erysipelothrix rhusiopathiae in farmed cattle indicates a low prevalence in dairy and feedlot cattle in the United States of America and Great Britain

Atienzar¹,

Gerber²,

Opriessnig³

2019

Preprint

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Background Clinical cases of Erysipelothrix rhusiopathiae, a zoonotic gram-positive bacterium, have been reported in many ruminant species, including in cattle, deer, moose and muskoxen. Fatal cases have been repeatedly reported in cattle over the years but to date there is only one Japanese study investigating the seroprevalence of this bacterium in bovine using the growth agglutination test (GAT). This technique is subjective, time-consuming, expensive and hazardous compared to modern serological tests such as enzyme-linked immunosorbent assays (ELISA) or the newly developed fluorescent microbead-based immunoassays (FMIA). Results A FMIA based on the surface protein SpaA (rSpaA415) antigen of E. rhusiopathiae developed in this study had an almost perfect agreement with the GAT (k=0.83) and showed a sensitivity of 89.7% and a specificity of 92.9% when compared to the GAT. Overall, detection rates of E. rhusiopathiae antibody positive samples were 13.8% (51/370) in British herds and 6% (12/200) in US herds. Positive cattle were present in 34.3% (24/70) of the investigated British farms and in 34.7% (8/23) of the US farms with an on-farm prevalence of 7.1% to 100% for the British and 8.3-30% for the US. Conclusions FMIA is a fast, safe and economic alternative to the GAT for the diagnosis of E. rhusiopathiae in cattle. This work is the first seroprevalence study of E. rhusiopathiae in healthy farmed cattle in the Great Britain and the US and revealed that infection occurs at a low level. Further investigations to evaluate risks of zoonotic transmission when handling cattle are needed.

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Section: Introductionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Use of the rSpaA415 antigen for detection of IgG anti-Erysipelothrix rhusiopathiae in farmed cattle indicates a low prevalence in dairy and feedlot cattle in the United States of America and Great Britain

Atienzar¹,

Gerber²,

Opriessnig³

2019

Preprint

View full text Add to dashboard Cite

show abstract

“…The same study also found a higher rate of seropositive cattle in areas also having swine industry [3]. This data could indicate that Erysipelothrix is mainly transmitted by pigs although cattle may also act as a vehicle for its distribution [5, 16]. In support of this, Erysipelothrix was isolated from cattle slurry [3] which could enhance the bacterium’s ability to spread as Erysipelothrix can survive in soil contaminated with faecal material [4].…”

Section: Introductionmentioning

confidence: 89%

“…During studies in Japanese abattoirs, Erysipelothrix was isolated from 6.4% of 1236 healthy, slaughtered cattle [16] which demonstrates that cattle may be subclinically infected with the bacterium. A follow-up epidemiological study using the growth agglutination test (GAT) to detect anti- Erysipelothrix antibodies in Japanese cattle found that 76% of 854 healthy cattle had detectable antibodies [3].…”

Section: Introductionmentioning

confidence: 99%

Use of the rSpaA415 antigen indicates low rates of Erysipelothrix rhusiopathiae infection in farmed cattle from the United States of America and Great Britain

2019

View full text Add to dashboard Cite

BackgroundClinical cases of Erysipelothrix rhusiopathiae, a zoonotic gram-positive bacterium, have been reported in many ruminant species, including in cattle, deer, moose and muskoxen. Fatal cases have been repeatedly reported in cattle over the years but to date there is only one Japanese study investigating the seroprevalence of this bacterium in cattle using the growth agglutination test (GAT). This technique is subjective, time-consuming, expensive and hazardous compared to modern serological tests such as enzyme-linked immunosorbent assays (ELISA) or the newly developed fluorescent microbead-based immunoassays (FMIA).ResultsThe FMIA based on the surface protein SpaA (rSpaA415) antigen of E. rhusiopathiae developed in this study had an almost perfect agreement with the GAT (k = 0.83) and showed a sensitivity of 89.7% and a specificity of 92.9% when compared to the GAT. Overall, detection rates of E. rhusiopathiae antibody positive samples were 13.8% (51/370) in British herds and 6% (12/200) in US herds. Positive cattle were present in 34.3% (24/70) of the investigated British farms and in 34.7% (8/23) of the US farms with an on-farm prevalence of 7.1 to 100% for the British farms and 8.3–30% for the US farms.ConclusionsFMIA is a fast, safe and economic alternative to the GAT for the diagnosis of E. rhusiopathiae in cattle. This work is the first seroprevalence study of E. rhusiopathiae in healthy farmed cattle in Great Britain and the US and revealed that infection occurs at a low level. Further investigations to evaluate risks of zoonotic transmission when handling cattle are needed.

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“…However, these methods are time-consuming and laborious because they require at least 2-4 days for isolation of Erysipelothrix spp. and about 6-8 days to determine its serotype (Hassanein et al 2000(Hassanein et al , 2001. Recently, several conventional PCR assays (Makino et al 1994;Shimoji et al 1998;Takeshi et al 1999;Yamazaki 2006) have been proposed that can replace the traditional time-consuming methods of routine culture detection.…”

mentioning

confidence: 99%

Rapid detection and differentiation of Erysipelothrix spp. by a novel multiplex real‐time PCR assay

Pal

Bender

Opriessnig

2010

Journal of Applied Microbiology

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Aim: The aim of this study was to develop a multiplex real‐time PCR assay for the identification and discrimination of Erysipelothrix rhusiopathiae, tonsillarum and Erysipelothrix sp. strain 2 for direct detection of Erysipelothrix spp. from animal specimens. Methods and Results: A primer set and three species‐specific probes with different end labelling were designed from the noncoding region downstream of the 5S rRNA coding region. The sensitivity, specificity and repeatability of the assay were validated by analysing 27 Erysipelothrix spp. reference serotype strains and ten septicemia‐associated non‐Erysipelothrix spp. bacterial isolates. Cross‐reactivity with Erysipelothrix sp. strain 1 was not observed with any of the primer probe combinations. The detection limit was determined to be <10 colony forming units and as low as one genome equivalent per PCR . Further evaluation of the Erysipelothrix spp. multiplex PCR was performed by comparing an enrichment isolation culture method and a conventional differential PCR on 15 samples from pigs experimentally inoculated with Erysipelothrix spp. and 22 samples from pigs with suspected natural infection. Conclusion: The multiplex real‐time PCR assay was found to be simple, rapid, reliable, specific and highly sensitive. Significance and Impact of the Study: The developed real‐time multiplex PCR assay does not require cumbersome and lengthy cultivation steps prior to DNA extraction, obtained comparable results to enrichment isolation, and will be useful in diagnostic laboratories for rapid detection of Erysipelothrix spp.

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Prevalence of Erysipelothrix Species in Healthy, Slaughtered Cattle.

Cited by 8 publications

References 10 publications

Use of the rSpaA415 antigen for detection of IgG anti-Erysipelothrix rhusiopathiae in farmed cattle indicates a low prevalence in dairy and feedlot cattle in the United States of America and Great Britain

Use of the rSpaA415 antigen for detection of IgG anti-Erysipelothrix rhusiopathiae in farmed cattle indicates a low prevalence in dairy and feedlot cattle in the United States of America and Great Britain

Use of the rSpaA415 antigen indicates low rates of Erysipelothrix rhusiopathiae infection in farmed cattle from the United States of America and Great Britain

Rapid detection and differentiation of Erysipelothrix spp. by a novel multiplex real‐time PCR assay

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