Prevalence of Enterocytozoon bieneusi and genetic diversity of ITS genotypes in sheep and goats in China

Zhao, Wei; Zhang, Weizhe; Yang, Dong; Zhang, Longxian; Wang, Rongjun; Liu, Aiqin

doi:10.1016/j.meegid.2015.03.026

Cited by 56 publications

(50 citation statements)

References 37 publications

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“…The phylum Microsporidia contains approximately 1300 species in 160 genera, and at least 17 species within nine genera have been identified in humans [2]. Enterocytozoon bieneusi is the most common microsporidian species, and is responsible for more than 90 % of reported cases of human microsporidiosis [3].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China

Deng

Zhong

et al. 2016

Parasites Vectors

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Background Enterocytozoon bieneusi is one of the most prevalent causative species of diarrhea and enteric diseases in various hosts. E. bieneusi has been identified in humans, mammals, birds, rodents and reptiles in China, but few studies have reported E. bieneusi in horses. Therefore, the present study was conducted to assess the prevalence, molecular characteristics and zoonotic potential of E. bieneusi among horses in southwestern China.FindingsThree hundred and thirty-three fecal specimens were collected from horses on five farms in the Sichuan and Yunnan provinces of southwestern China. The prevalence of E. bieneusi was 22.5 % (75/333), as determined by nested polymerase chain reaction and sequencing analysis of the internal transcribed spacer region of the ribosomal RNA gene of E. bieneusi. Altogether, 10 genotypes were identified among the 75 E. bieneusi-positive samples: four of these genotypes were known (horse1, horse2, SC02 and D) and six were novel (SCH1-4 and YNH1-2). Multilocus sequence typing using three microsatellites (MS1, MS3 and MS7) and one minisatellite (MS4) revealed three, two, three and three genotypes at these four loci, respectively. In phylogenetic analysis, all the genotypes of E. bieneusi obtained in this study were clustered into three distinct groups: D, SC02 and SCH1-3 were clustered into group 1 (zoonotic potential); SCH4 was clustered into group 2 (cattle-hosted); whereas horse2, YNH1 and YNH2 were clustered into group 6 (unclear zoonotic potential).ConclusionsThis is the first report of E. bieneusi among horses in southwestern China. This is also the first multilocus genotyping analysis using microsatellite and minisatellite markers of E. bieneusi in horses. The presence of genotype D, which was previously identified in humans, and genotypes SC02 and SCH1-3, which belong to potential zoonotic group 1, these results indicate that horses are a potential source of human E. bieneusi infections in China.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China

Deng

Zhong

et al. 2016

Parasites Vectors

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“…Group 1 strains have zoonotic potential, while strains belonging to groups 2–9 are associated with animals or wastewater (Santin and Fayer ; Zhao et al. ). Enterocytozoon bieneusi isolates belonging to more than 60 different genotypes have been identified in pigs, with most also found in humans (Li et al.…”

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confidence: 99%

“…Based on phylogenetic analyses, E. bieneusi strains have been divided into nine different groups. Group 1 strains have zoonotic potential, while strains belonging to groups 2-9 are associated with animals or wastewater (Santin and Fayer 2011;Zhao et al 2015). Enterocytozoon bieneusi isolates belonging to more than 60 different genotypes have been identified in pigs, with most also found in humans Santin and Fayer 2011;Zhang et al 2011;Zhao et al 2014).…”

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Multilocus Typing of Enterocytozoon bieneusi in Pig Reveals the High Prevalence, Zoonotic Potential, Host Adaptation and Geographical Segregation in China

Zheng

Zhou

et al. 2019

J Eukaryotic Microbiology

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Enterocytozoon bieneusi is one of the most frequently diagnosed Microsporidia of humans and most animals. However, there is no information on E. bieneusi infection of pigs in Tibet and Henan, China. In this study, 1,190 fecal samples were collected from pigs in Tibet and Henan and screened for the presence of E. bieneusi. The overall prevalence of E. bieneusi infection was 54.2% (645/1,190), with differences in prevalence observed among geographical areas, ages, and pig breeds. Moreover, 10 E. bieneusi genotypes were identified based on internal transcribed spacer region genotyping, including eight known genotypes (EbpC, EbpA, CHG19, CHC5, Henan‐III, I, D, and H) and two novel genotypes (XZP‐I and XZP‐II). Multilocus sequence typing revealed 18, 7, 17, and 13 genotypes at minisatellite/microsatellite loci MS1, MS3, MS4, and MS7, respectively. Strong linkage disequilibrium (LD) and few numbers of recombination events, suggest a clonal structure of the E. bieneusi population examined in this study. The low pairwise genetic distance (FST) and gene flow (Nm) values indicated limited gene flow in the E. bieneusi population from different hosts, with phylogenetic, structure, and median‐joining network analyses all indicating the existence of host and geographical isolation. The identification of isolates belonging to nine human‐pathogenic genotypes indicates that pigs play an important role in the dissemination of E. bieneusi, improving our present understanding of E. bieneusi epidemiology in the studied region.

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“…; Santín and Fayer ; Zhao et al. ). Enterocytozoon bieneusi is mainly transmitted through the fecal‐oral route, and has been identified in water sources as well as in wild, domestic, and farm animals, raising concerns about its zoonotic transmission (Guo et al.…”

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confidence: 99%

“…The genotyping of E. bieneusi using sequencing analyses of the ribosomal internal transcribed spacer (ITS) has identified more than 200 genotypes in a wide range of host species, including humans (Perec-Matysiak et al 2015;Yang et al 2014;Zhao et al 2015c). To date, at least 60 genotypes have been detected in humans, 33 of which are also found in animals, suggesting the potential zoonotic transmission of E. bieneusi (Perec-Matysiak et al 2015;Sant ın and Fayer 2011;Zhao et al 2015a). Enterocytozoon bieneusi is mainly transmitted through the fecaloral route, and has been identified in water sources as well as in wild, domestic, and farm animals, raising concerns about its zoonotic transmission (Guo et al 2014).…”

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Enterocytozoon bieneusi Genotypes in Grazing Horses in China and their Zoonotic Transmission Potential

Wang

et al. 2016

J Eukaryotic Microbiology

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In present study, 262 fecal specimens were collected from 12 groups of grazing horses in the Xinjiang Uyghur Autonomous Region of China. The specimens were subjected to PCR and sequencing analyses of the ribosomal internal transcribed spacer (ITS). The overall prevalence of E. bieneusi in horses was 30.9% (81/262). No significant differences in prevalence were observed between horses of different ages or sexes. Nineteen genotypes were identified: 15 known genotypes (BEB6, CHG19, CM6, CM7, CM8, CS-1, CS-4, D, EpbA, EbpC, G, horse1, horse2, O, and Peru8) and four new genotypes (XJH1-XJH4). Six of these genotypes were previously detected in humans: BEB6, D, EbpA, EbpC, O, and Peru8. Genotype EbpC was the most prevalent (21/81), followed by EpbA (20/81), BEB6 (9/81), CM6 (4/81), horse1 (4/81), O (4/81), G (3/81), CHG19 (2/81), CM7 (2/81), horse2 (2/81), and XJH1 (2/81), whereas the remaining eight genotypes were seen in one specimen each. In a phylogenetic analysis, 14 genotypes (65/81, 80.2%), excluding genotypes BEB6, CM7, horse2, XJH1, and XJH4, belonged to group 1, which have zoonotic potential. The high diversity in the E. bieneusi genotypes and their zoonotic potential suggest that grazing horses are a potential source of zoonotic infection in humans.

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Prevalence of Enterocytozoon bieneusi and genetic diversity of ITS genotypes in sheep and goats in China

Cited by 56 publications

References 37 publications

Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China

Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China

Multilocus Typing of Enterocytozoon bieneusi in Pig Reveals the High Prevalence, Zoonotic Potential, Host Adaptation and Geographical Segregation in China

Enterocytozoon bieneusi Genotypes in Grazing Horses in China and their Zoonotic Transmission Potential

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