Prevalence of Enterococcus faecalis at Multiple Oral Sites in Endodontic Patients Using Culture and PCR

Sedgley, Christine M.; Buck, Gwendolyn; Appelbe, Oliver K.

doi:10.1016/j.joen.2005.10.022

Cited by 98 publications

(102 citation statements)

References 35 publications

(43 reference statements)

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“…E. faecalis is the most often retrieved pathogen in asymptomatic endodontic infections because of its capacity to invade dentinal tubules, compete with other microorganisms and resist nutritional privation. It is a facultative anaerobic Gram-positive coccus that may present either isolated, in pairs or in chains (4,16,17,20).…”

Section: Discussionmentioning

confidence: 99%

Influence of the length of remaining root canal filling and post space preparation on the coronal leakage of Enterococcus faecalis

Mozini¹,

Vansan²,

Sousa‐Neto³

et al. 2009

Braz. J. Microbiol.

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This study evaluated the sealing ability of different lengths of remaining root canal filling and post space preparation against coronal leakage of Enterococcus faecalis. Forty-one roots of maxillary incisors were biomechanically prepared, maintaining standardized canal diameter at the middle and coronal thirds. The roots were autoclaved and all subsequent steps were undertaken in a laminar flow chamber. The canals of 33 roots were obturated with AH Plus sealer and gutta-percha. The root canal fillings were reduced to 3 predetermined lengths (n=11): G1=6 mm, G2=4 mm and G3=2 mm. The remaining roots served as positive and negative controls. Bacterial leakage test apparatuses were fabricated with the roots attached to Eppendorf tubes keeping 2 mm of apex submerged in BHI in glass flasks. The specimens received an E. faecalis inoculum of 1 x 107 cfu/mL every 3 days and were observed for bacterial leakage daily during 60 days. Data were submitted to ANOVA, Tukey's test and Fisher's test. At 60 days, G1 (6 mm) and G2 (4 mm) presented statistically similar results (p>0.05) (54.4% of specimens with bacterial leakage) and both groups differed significantly (p<0.01) from G3 (2 mm), which presented 100% of specimens with E. faecalis leakage. It may be concluded that the shortest endodontic obturation remnant leaked considerably more than the other lengths, although none of the tested conditions avoids coronal leakage of E. faecalis.

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Section: Discussionmentioning

confidence: 99%

Influence of the length of remaining root canal filling and post space preparation on the coronal leakage of Enterococcus faecalis

Mozini¹,

Vansan²,

Sousa‐Neto³

et al. 2009

Braz. J. Microbiol.

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“…In fact the detection limit of cultivation-based PCR was improved by initial culturing of the clinical samples. Recent reports have indicated the superiority of PCR, quantitative real time PCR and reverse transcription PCR over cultivation for detection of E. faecalis, as the latter often produces false negative results (20,21,25).…”

Section: Discussionmentioning

confidence: 99%

“…PCR-based diagnosis of E. faecalis has been reported by many workers (14)(15)(16)(17)(18)(19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and identification of Enterococcus faecalis from necrotic root canals using multiplex PCR

et al. 2007

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This study was designed to survey the incidence of Enterococcus faecalis infection in symptomatic and asymptomatic root canals of necrotic teeth using PCR and to isolate the bacterium for further screening. Sixty patients categorized according to their clinical symptoms were used for sampling by insertion of paper points into the root canals and absorbing all the fluids present within them. The samples were incubated in 1.0 ml 2xYT (containing 16 g bacto tryptone, 10 g yeast extract and 5.0 g NaCl per liter) for 24 h at 37°C without aeration prior to multiplex PCR analysis. To assist the isolation of E. faecalis, subsamples were further grown in the same medium supplemented with 6.5% NaCl and back-inoculated into bile esculin. Using multiple cultivation-dependent and PCR analyses, 6 cases (10%) of E. faecalis were identified. Four isolates were obtained from asymptomatic cases of chronic apical periodontitis, and the other two were associated with phoenix abscess and acute apical abscess, respectively. No E. faecalis infection was found in 5 patients with acute apical periodontitis or in 9 with chronic suppurative periodontitis. Our results indicate that there is no significant difference in the incidence of E. faecalis between symptomatic and asymptomatic necrotic dental root canals (P > 0.05). (J. Oral Sci. 49, [221][222][223][224][225][226][227] 2007)

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“…Enterococcus faecalis é comumente encontrado em lesões periapicais persistentes e seu papel na indução da doença periapical ainda não é claramente elucidado, por isso a importância da escolha pelo LTA (Sedgley et al, 2006;Roças;. Especula-se que a resposta celular a LTA seja desencadeada a partir da interação desta molécula com receptores de imunidade inata (Toll-like receptor TLR2/ TLR6 (Jin et al, 2007), comumente presentes em células residentes de tecidos conjuntivos (Morandini et al, 2013).…”

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Influência de medicações intracanal utilizadas em procedimentos endodônticos regenerativos na sobrevivência de células da papila apical in vitro

Oliveira¹

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Oliveira AP. Influence of intracanal medications used in regenerative endodontic procedures on survival of apical papilla cells in vitro [thesis]. São Paulo: Universidade de São Paulo, Faculdade de Odontologia; 2014. Versão Corrigida.Regenerative Endodontic procedures have provided changes in treatment of patients with immature teeth and apical periodontitis enabling full root development and lower incidence of dental fracture. Intracanal dressings are used for disinfection; however, their effect on apical papilla cells is poorly elucidated. Additionally, the effect of these substances on cells previously subjected to proinflammatory condition is still unknown. The aim of this study was to evaluate the cytotoxicity of intracanal dressings used in Endodontics regenerative procedures on cultured human apical papilla cells at physiologic and activated condition. Cell culture was established from the apical papilla removed from an extracted immature third molar. The substances studied were triple antibiotic modified paste: ciprofloxacin, metronidazole and cephalosporin (1:1:1); CFC: ciprofloxacin, metronidazole and calcium hydroxide (1:1:2) and modified CFC: ciprofloxacin , metronidazole and calcium hydroxide (2:2:1). Part of the cells was stimulated previously with lipotheichoic acid (LTA) of Enterococcus faecalis por 7 days. Once plated, cells were exposed to increasing concentration of the medications for 1, 3, 5 and 7 days. Cell viability was evaluated by means of 3-bromide (4.5-dimetiliazol-2-yl) -2.5-difeniltetraze (MTT) and Nitric Oxide (NO) release was assessed by the Griess method. The statistical analysis was done through analysis of variance with 1 criteria (ANOVA) followed by Tukey test with 5% of significance level. Modified CFC was the medication that demonstrated the less cytotoxic effect on cell viabilityat the experimental periods studied while CFC promoted significant decrease on cell viability specially after 7 days of contact. The modified triple antibiotic paste resulted in important alteration of cell viability being considered the most citotoxic. Cellular activation by LTA resulted in increased levels of mitochondrial activity for all medications being more evident at the longer experimental periods. Cellular activation also contributed to higher levels of nitric oxide release. In conclusion, the cytotoxic effect of the tested medications is dependent on concentration, time of contact and cellular condition, being the triple antibiotic modified paste the most cytotoxic in high concentrations leading clinically in the decreased of the cells viability of the apical papilla, decreasing the success of regenerative procedures.

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Prevalence of Enterococcus faecalis at Multiple Oral Sites in Endodontic Patients Using Culture and PCR

Cited by 98 publications

References 35 publications

Influence of the length of remaining root canal filling and post space preparation on the coronal leakage of Enterococcus faecalis

Influence of the length of remaining root canal filling and post space preparation on the coronal leakage of Enterococcus faecalis

Isolation and identification of Enterococcus faecalis from necrotic root canals using multiplex PCR

Influência de medicações intracanal utilizadas em procedimentos endodônticos regenerativos na sobrevivência de células da papila apical in vitro

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