Prevalence of Dermatophytic Infection and Detection of Dermatophytes by Microscopic and Culture Methods

Islam, Tashmin Afroz Binte; Majid, Farjana; Ahmed, Mushtaque; Afrin, Sonya; Jhumky, Tahmina; Ferdouse, Faria

doi:10.3329/jemc.v8i1.35429

Cited by 13 publications

(13 citation statements)

References 13 publications

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“…10 In present study 28.9% cases were found as culture positive which are comparable with previous studies. 3,8 This is slightly lower than 43%. 11 Higher or lower isolation rates from clinical specimens depends on different factors like collection of specimens, composition and pH of the media, incubation period and temperature, viability of fungal elements and co-existing microbes which may inhibit the growth of pathogenic fungi.…”

Section: Discussionmentioning

confidence: 76%

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Detection of Drug Susceptibility to Azoles among Trichophyton rubrum Isolates by Disk Diffusion Method

Mohiuddin¹,

Sattar²,

Saleh³

et al. 2019

Bangladesh J Med Microbiol

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Dermatophytes are filamentous fungi that cause tiniasis with high prevalence in tropical hot, humid and overcrowded countries like Bangladesh. Antifungal drug resistance among dermatophyte isolates are very common due to irrational and overuse of them. In vitro antifungal susceptibility test may help to select appropriate drug, optimize the therapy and monitor the emergence of drug resistance. This study was undertaken to isolate and identify different dermatophyte species from skin, nail and hair samples and to see the susceptibility pattern of azoles (fluconazole, miconazole and itraconazole) among one of the most common dermatophyte isolates (Trichophyton rubrum) by disk diffusion method. A total of two hundred and forty six patients clinically suspected of dermatophytosis attending in the outpatient department (OPD) of Dermatology and Venereology of BSMMU, Dhaka, were studied from September 2018 to August 2019. All the samples of skin, nail and hair were processed for direct microscopic examination and culture. The species of dermatophytes were identified by gross colony morphology, microscopic features and biochemical tests. Antifungal susceptibility was performed by disk diffusion (Neo-Sensitabs susceptibility testing modified for Dermatophytes) method in Sabouraud’s dextrose agar (SDA) media. Out of 246 specimens 27.2% were positive by microscopy, 28.9% were positive by culture and 26.0% were positive by both microscopy and culture. Tinea cruris (32.4%) was the most prevalent clinical type among all dermatophyte isolates. Among 71 culture positive isolates, T. rubrum (71.8%) was most prevalent followed by T. mentagrophytes (24.0%) and E. floccosum (4.2%). By disk diffusion method fluconazole was found as the most resistant drug (90.2%) against T. rubrum isolates and Itraconazole was the most sensitive (80.4%). Miconazole was found as most intermediate sensitive. Bangladesh J Med Microbiol 2019; 13 (1): 20-23

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Section: Discussionmentioning

confidence: 76%

“…Among 246 specimens 27.2% were positive by microscopic examination which is comparable with other studies. 3,8 This result is considered as higher than 14.3% 9 and lower compared to 45.5%. 10 In present study 28.9% cases were found as culture positive which are comparable with previous studies.…”

Section: Discussionmentioning

confidence: 82%

Detection of Drug Susceptibility to Azoles among Trichophyton rubrum Isolates by Disk Diffusion Method

Mohiuddin¹,

Sattar²,

Saleh³

et al. 2019

Bangladesh J Med Microbiol

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“…Similarly, other studies from Karachi and Bangladesh reported 26 (20.97%) and 31 (38.75%) cases of dermatophytes positive on culture respectively. 11,12 According to another study conducted by Majeed et al 13 a total of one hundred and fifty suspected cases of dermatophytosis were studied, out of which 99 (66%) cases were positive by direct microscopy while only 74 (49.33%) were positive by culture. Pandit and Mehta found 32.1% positive cases on culture out of n = 530 patients with clinically diagnosed dermatophytoses.…”

Section: Dermatophytesmentioning

confidence: 99%

“…On the other hand, samples with negative microscopic results but positive cultures can be explained by the fact that the fungus might be in an inactive sporulating phase of sporulation that might have been difficult to detect by direct microscopy but can grow into molds when inoculated on culture media. 12 The objective of this study was to compare DTM as a screening medium in comparison with SDA to have a better idea of the efficacy of DTM for early identification and isolation of dermatophytes. SDA is the most used culture medium for the primary isolation of dermatophyte species.…”

Section: Dermatophytesmentioning

confidence: 99%

Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species

Ahmad

Javed

Mushtaq³

et al. 2020

BIOMEDICA

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Background and Objective: Dermatophyte infections require laboratory diagnosis before treatment is started. Although direct microscopy is routinely performed but culture of dermatophytes is the gold standard. However, it takes about 4 weeks for species identification on primary media. Our aim was to compare dermatophyte test medium (DTM) as a screening medium for the isolation of dermatophytes in comparison with sabouraud dextrose agar (SDA). Methods: It was a comparative study carried out at the Department of Microbiology of Post Graduate Medical Institute, Lahore over a period of nine months. Samples were collected from one hundred patients with clinically suspected dermatophytoses after taking informed written consent. The samples were examined microscopically and then inoculated on two types of culture media, one Sabouraud dextrose agar (SDA) with added chloramphenicol, gentacin and cycloheximide and other dermatophyte test medium (DTM) with added chlortetracycline, gentacin and cyclohexamide. Results: Fungal growth was observed in fifty-six samples on culture. Out of the fifty-six positive on cultures, nineteen were that of dermatophytes. Out of n = 100 patients, ten were positive on SDA while n = 14 dermatophyte species were able to grow on DTM. A significantly higher positivity (P ³ 0.05) for isolating dermatophytes was observed by DTM as compared to SDA. DTM was able to isolate (71%) of the dermatophytes in first 10 days. Isolation rate of dermatophyte species was higher (73.68%) on DTM as compared to SDA which was 52.6%. Conclusion: Authors recommend the use of dermatophyte test medium for the primary isolation and identification of dermatophyte species to be more effective and time saving.

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“…The Dermatophytosis can easily be diagnosed based on the history, physical examination, and laboratory diagnosis. The laboratory diagnosis of Dermatophytosis routinely involves Potassium Hydroxide (KOH) Mount and direct microscopic examination of clinical specimen followed by in vitro fungal culture techniques [5]. With the advent of molecular technology, the techniques like polymerase chain reaction which is a highly sensitive and specific test can be used for confirmation of diagnosis of fungal Infections [5].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Dermatophytosis in South Indian Populations: A Research Hospital Based Study

Fatima

Ahmad

Latha

et al. 2021

IJTDH

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Aim: Dermatophytosis is a superficial fungal infection distributed worldwide, more prevalent in the tropical countries like India. Dermatophytosis is diagnosed based on the history, physical examination, and laboratory diagnosis by Microscopic examination of Potassium Hydroxide (KOH) Mount followed by fungal culture. KOH mount is a rapid, simple, time saving and cost effective diagnostic method. The present study was conducted to know the prevalence and common clinical presentations of Dermatophytosis in Hyderabad, South India. Materials and Methods: The study was carried out in Clinical Microbiology section of the Laboratory of the Skin Research Institute in Hyderabad, South India, for a period of 1 year from August 2020 to September 2021. A total of 236 skin scarping samples from clinically suspected cases of dermatophytosis were collected. A detailed clinical history of the patients was recorded and skin scrapping samples was subjected to KOH test. Results: Of all the 236 patients, 186 patients (78.81%) were positive of fungal elements. Males (58.06 %) were more commonly affected than females (41.94%). Tinea corporis was the most common clinical type (50%) followed by Tinea Cruris (22.58%). The microscopic finding revealed 49.46% of samples showing long, septate hyphae, 39.78% of samples showing Spaghetti and meatball appearance and 10.75% of samples showing pseudohyphae with budding yeast cells forms. Conclusions: The prevalence of Dermatophytosis is high in the study population. In experienced hands, a potassium hydroxide mount is one of the most useful procedures in medical mycology and can be used as screening test and routine investigation in the diagnosis of dermatophytosis so that definite treatment can be commenced without delay.

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Prevalence of Dermatophytic Infection and Detection of Dermatophytes by Microscopic and Culture Methods

Cited by 13 publications

References 13 publications

Detection of Drug Susceptibility to Azoles among Trichophyton rubrum Isolates by Disk Diffusion Method

Detection of Drug Susceptibility to Azoles among Trichophyton rubrum Isolates by Disk Diffusion Method

Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species

Prevalence of Dermatophytosis in South Indian Populations: A Research Hospital Based Study

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