Prevalence of Class I and II Integrons among MDR Enterobacter cloacae Isolates Obtained from Clinical Samples of Children in Kermanshah, Iran

Mortazavi, Seyed Hamidreza; Mansouri, Faizullah; Azizi, Mohsen; Alvandi, Amirhooshang; Karbasfrushan, Ali; Madadi-Goli, Nahid; Fereshteh, Sepideh; Ahmadi, Kamal

doi:10.7860/jcdr/2018/37826.12396

Cited by 5 publications

(6 citation statements)

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“…However, a significant relationship was revealed between the presence of the intI2 gene and higher rates of drug resistance against only two antibiotics (Table 3). In line with these results, Mortazavi et al (2018) showed that 58.3% of E. cloacae isolates harbored class I integron; however, none of them had class II integron [40] . In the present research, the intI3 gene was not detected in any of the isolates.…”

Section: Discussionsupporting

confidence: 72%

Emergence of OXA-10 and OXA-48 Like Carbapenemases among Enterobacter Isolates from Inpatients in Namazi Hospital in Shiraz

Moradi

Khashei²,

Malekzadegan³

et al. 2022

IEM

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Backgrounds:The global spread of carbapenemase-producing Enterobacteriaceae represents a public health concern. This study aimed to investigate the prevalence of carbapenem resistance and the presence of some oxacillinase types and class 1-3 integrons among Enterobacter clinical isolates from an Iranian inpatient population. Materials & Methods: Ninety Enterobacter isolates from hospitalized patients were diagnosed by microbiological methods. Antibiogram pattern was also determined. The presence of class 1-3 integrons and four types of oxacillinase genes was assessed using PCR. Findings: Among 90 Enterobacter isolates, the most common species was E. aerogenes, (45.6%), followed by E. cloacae (30%). The highest resistance rate was against ampicillin (96.7%). Multidrug resistance (MDR) was substantial (93%). Carbapenemase-producers were detected in 96% of carbapenem-resistant isolates by mCIM test. The frequency of evaluated genes was as follows: intI1 = 50 (55.6%), intI2 =12 (13.3%), bla oxa-1 =6 (6.7%), bla oxa-2 =5 (5.6%), bla oxa-10 =18 (20%), and bla oxa-48 =18 (20%). Conclusion: Determinants of class 1 integron along with OXA-10 and OXA-48 like carbapememases are responsible for relatively considerable carbapenem resistance among isolates. This is the first report about the presence of OXA-10 and OXA-48-producing Enterobacter spp. in Iran, indicating that the prevalence of oxacillinases in the country might be on the rise.

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Section: Discussionsupporting

confidence: 72%

Emergence of OXA-10 and OXA-48 Like Carbapenemases among Enterobacter Isolates from Inpatients in Namazi Hospital in Shiraz

Moradi

Khashei²,

Malekzadegan³

et al. 2022

IEM

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“…The frequency of Class 1 integron was 64% in this s1tudy, which is supported by the results of other studies conducted in Iran, while Class 2 integron was not detected. There is no evidence about the presence of Class 2 integron among E. cloacae isolates in published studies (1,30) . According to the statistical analysis, the correlation between harboring Class 1 integron and resistance to streptomycin and ciprofloxacin was significant (p value< .05), while resistance to other antibiotics was not correlated with harboring Class 1 integron, and other resistance mechanisms should be responsible for resistance.…”

Section: Discussionmentioning

confidence: 99%

“…Low prevalence of tetracycline resistance genes in phenotypically resistant isolates could be due to the presence of other resistance mechanisms, including integrons or genes which has not yet been detected in E. cloacae strains. The prevalence of MDR E. cloacae strains demonstrated an upward trend in the emergence of MDR strains in the clinical samples in Iran in such a way that the rate has increased from zero in 2012 to 75% in 2017 according to the published data [27,[30][31] . Integrons are mostly responsible for the transmission of resistance determinants within species or among different bacterial species or genera.…”

Section: Discussionmentioning

confidence: 99%

Tetracycline Resistance Associated with tet Genes or Integrons among Enterobacter cloacae Strains Isolated from Patients with Urinary Tract Infections

Majidpour

Boustanshenas

Banihashem

et al. 2020

IEM

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Background: This study aimed to evaluate the prevalence of tet genes and Class I and 2 integrons in Enterobacter cloacae strains isolated from patients with urinary tract infections (UTIs). Materials & Method: A total of 50 E. cloacae isolates were collected. Antimicrobial susceptibility pattern and tetracycline MIC were determined. The presence of tet genes (tetA, tetB, tetC, tetD) and Class 1 and 2 integrons and the content of Class 1 integron were determined. Findings: Tetracycline MIC pattern classified 36 % of the E. cloacae isolates as resistant. The most common tet gene was tetC (22%), followed by tetD, tetA, and tetB. Class 1 integron was detected in 64% of the isolates. Class 1 integron content analysis showed two variable gene cassettes (aadA1 and aadA5/dfrA17 genes). The frequency of aadA5/dfrA17 was 18.75%, which was more common than aadA1 gene (6.25%). Conclusion: The most important genetic markers for tetracycline resistance in E. cloacae isolates were tetC and Class 1 integron. Harboring Class 1 integron and resistance to streptomycin and ciprofloxacin were significantly correlated.

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“…E. cloacae and Enterobacter aerogenes have been identified as major nosocomial pathogens among these bacterial species. E. cloacae is responsible for over 70% of these bacterial infections [ 2 ]. Due to various virulence factors, such as biofilm-forming ability, toxins, cytotoxicity, and hemolysin release, this bacterium can lead to several nosocomial infections, including pneumonia, urinary tract infections, surgical wounds, skin and soft tissue infections, and bacteremia [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

The Abundance of Plasmid-Mediated Quinolone Resistance Genes in Enterobacter cloacae Strains Isolated from Clinical Specimens in Kermanshah, Iran

Afsharian,

Asadi,

Danesh

et al. 2024

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Background. Enterobacter cloacae (E. cloacae) is one of the most common Enterobacteriaceae causing nosocomial infections. Plasmid-mediated quinolone resistance (PMQR) determinants have been considered recently. This study evaluated the abundance of PMQR genes in strains of E. cloacae obtained from clinical samples in Kermanshah, Iran. Methods. In this descriptive cross-sectional study, after collecting 113 isolates of E. cloacae, their identity was confirmed using specific biochemical tests. After determining their drug resistance patterns using disc diffusion, the phenotypic frequency of extended-spectrum beta-lactamase (ESBL)-producing isolates was measured by the double-disk synergy test (DDST) method. The isolates were examined for the presence of qnrA, qnrB, qnrS, and aac(6′)-Ib-cr genes by the polymerase chain reaction (PCR) assay. Results. The antibiotic resistance rate of E. cloacae isolates varied from 9.7% to 60.2%; among them, 78% were multidrug-resistant (MDR). The highest quinolone resistance was observed in ESBL-producing strains of E. cloacae. The frequency of positive isolates for PMQR and ESBL was 79.6% and 57.5%, respectively. The genes aac(6′)-ib-cr (70.8%) and qnrB (38.1%) had the highest frequency among other genes. The number of isolates simultaneously carrying 2 and 3 genes was 64 and 5 isolates, respectively. Conclusion. The obtained results indicate a high degree of quinolone resistance among ESBL-producing E. cloacae strains. Nevertheless, there was a significant relationship between the PMQR gene and ESBL-positive isolates. Therefore, special attention should be paid to molecular epidemiological studies on antibiotic resistance to quinolones and beta-lactamases in these strains.

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Prevalence of Class I and II Integrons among MDR Enterobacter cloacae Isolates Obtained from Clinical Samples of Children in Kermanshah, Iran

Cited by 5 publications

References 0 publications

Emergence of OXA-10 and OXA-48 Like Carbapenemases among Enterobacter Isolates from Inpatients in Namazi Hospital in Shiraz

Emergence of OXA-10 and OXA-48 Like Carbapenemases among Enterobacter Isolates from Inpatients in Namazi Hospital in Shiraz

Tetracycline Resistance Associated with tet Genes or Integrons among Enterobacter cloacae Strains Isolated from Patients with Urinary Tract Infections

The Abundance of Plasmid-Mediated Quinolone Resistance Genes in Enterobacter cloacae Strains Isolated from Clinical Specimens in Kermanshah, Iran

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