Prevalence of Capnocytophaga canimorsus and Capnocytophaga cynodegmi in dogs and cats determined by using a newly established species-specific PCR

Suzuki, Mizuto; Kimura, Masanobu; Imaoka, Koichi; Yamada, Akio

doi:10.1016/j.vetmic.2010.01.001

Cited by 77 publications

(81 citation statements)

References 16 publications

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“…55 PCR and 16S ribosomal ribonucleic acid (rRNA) sequencing can help correctly identify C. canimorsus, and to differentiate it from C. cynodegmi, which is less pathogenic. 54,56 Most of the human infections occur from dogs; however, serious disease requiring emergency care and prolonged hospitalization following a cat scratch in a 49-year-old alcoholic has been reported.…”

Section: Capnocytophaga Canimorsusmentioning

confidence: 99%

Zoonotic Bacteria Associated with Cats

Hariharan¹,

Hariharan²

2017

Vet Med Open J

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CitationHariharan H, Hariharan SH. Zoonotic bacteria associated with cats. Vet Med Open J. 2017; 2(3): 68-75. doi: 10.17140/VMOJ-2-118 ABSTRACT Background: There are several bacterial pathogens, including Bartonella, and Pasteurella that have been known to cause human infections from contact with cats. Recently, certain bacteria have emerged as causes of infection in cats, resulting in transmission to humans. Objectives: This review presents updated information on the zoonotic bacterial pathogens associated with cats, with special emphasis on recent reports. Results: Presently, an increase in the incidence of cat-associated Yersinia pestis human infections in the western USA has become a concern. In addition, infections due to Mycobacterium bovis, and Brucella abortus, are being recognized. Also, a number of human infections due to Campylobacter, Helicobacter, and Salmonella from cats have been documented worldwide. In addition, spread of bacteria resistant to antimicrobial drugs from cats is becoming a possibility. Conclusion: Updated information shows that more zoonotic bacteria than previously recognized are associated with cats worldwide. Immunocompromised persons and young children are particularly at a risk of developing infection from household cats which are allowed to roam around outdoors. It is important to incorporate environmental and animal contact considerations into patient care. With proper handling and precautions, cats can help establish valuable humananimal bonds.

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Section: Capnocytophaga Canimorsusmentioning

confidence: 99%

Zoonotic Bacteria Associated with Cats

Hariharan¹,

Hariharan²

2017

Vet Med Open J

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“…CaL2, AS1, CaR, and CyR primers were designed for the determination for Capnocytophaga spp which informed as Suzuki et al [12] .…”

Section: Primers and Pcrmentioning

confidence: 99%

“…; ViBuffer A (Vivantis®) 10x enzyme buffer solution 1x, magnesium chloride (MgCl 2 ) 50 mM, 10x dNTP, primer (for CaL2-AS1 primer pair) 10 pmol, Pfu DNA polymerase 5U (Vivantis®) [12] .…”

Section: Primers and Pcrmentioning

confidence: 99%

“…The second PCR amplifications were performed to in the same conditions using by CaL2-CaR and CaL2-CyR primers to identify C. canimorsus and C. cynodegmi [12] . PCR conditions were as follows; an initial denaturation at 95°C for 5 min, followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 58°C for 1 min, extension at 72°C for 1 min, and a final extension at 72°C for 7 min [12] .…”

Section: Primers and Pcrmentioning

confidence: 99%

“…PCR conditions were as follows; an initial denaturation at 95°C for 5 min, followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 58°C for 1 min, extension at 72°C for 1 min, and a final extension at 72°C for 7 min [12] . PCR was performed in an Eppendorf Master Cycler with a capacity of 25 samples thermal cycling.…”

Section: Primers and Pcrmentioning

confidence: 99%

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Detection of Capnocytophaga canimorsus and Capnocytophaga cynodegmi by Cultural and Molecular Methods in Dogs in Western Turkey

Özavcı¹,

Kırkan²

2013

Kafkas Univ Vet Fak Derg

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The aim of this study is to exhibit the presence of Capnocytophaga species in Turkey and to determine the regional risks of these bacteria for the people in dogs. Totally 200 oral swab samples were taken from owned dogs which have had dental plaque problems. The diagnosis of Capnocytophaga infections were done by PCR using CaL2, AS1, CaR and CyR gene sequence primers. The first PCR was carried out to samples using by CaL2 and AS1 primers and results were estimated as Capnocytophaga species. The second PCR was applied to samples using CaR and CyR reverse primers and results were also interpreted as being Capnocytophaga canimorsus and Capnocytophaga cynodegmi. At the end of the first PCR, Capnocytophaga spp. was detected from 11 (5.5%) out of 200 samples. CaR and CyR gene were investigated in samples which were detected as Capnocytophaga spp. It was determined that 2 (18.2%) of the samples were positive for CaR gene which were identified as C. canimorsus and 9 (81.8%) of the samples were positive for both CaR and CyR gene which were identified as C. canimorsus and C. cynodegmi. C. canimorsus and C. cynodegmi species are concluded for generating risk for human health and due to the lacking of information about the disease it was also difficult to diagnosis these agents.

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Bacterial Diseases

Weese

BSc

2010

Companion Animal Zoonoses

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Prevalence of Capnocytophaga canimorsus and Capnocytophaga cynodegmi in dogs and cats determined by using a newly established species-specific PCR

Cited by 77 publications

References 16 publications

Zoonotic Bacteria Associated with Cats

Zoonotic Bacteria Associated with Cats

Detection of Capnocytophaga canimorsus and Capnocytophaga cynodegmi by Cultural and Molecular Methods in Dogs in Western Turkey

Bacterial Diseases

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