Prevalence of Candida bloodstream isolates from patients in two hospitals in Vietnam

Bac, Nguyen Duy; Anh, Le Tran; Quang, Le Bach; Luc, Nguyen Khac; Nga, Tran Thi Thanh; Nagi, Minoru; Miyazaki, Yoshitsugu; Ha, Hoang Thi Thu; Anh, Đặng Đức; Đỗ, Quyết; Anh, Do Ngoc

doi:10.18502/ijm.v11i2.1071

Cited by 3 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…C. tropicalis is reported as one of the four major Candida species responsible for candidemia worldwide [39]. It has been described as the first species in Vietnam [12]. It has increased dramatically in the last years due to the development of resistance to fluconazole [34,40], especially in Asia than in North America or Europe [35,41], resulting in higher mortality compared to C. albicans [40].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Azole non-susceptible C. tropicalis and polyclonal spread of C. albicans in Central Vietnam hospitals

Ngo

Santona

Fiamma

et al. 2023

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Candida spp. are responsible for infections ranging from local to systemic, and resistance to antifungal first-line therapy is increasing in non-albicans Candida species. We aimed to determine the etiology of candidiasis and the antifungal resistance of Candida spp. isolated in Hue hospitals, Central-Vietnam. Methods: Species identification was performed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry supported by fungal internal-transcribed-spacer amplification and sequencing. Antifungal susceptibility testing was performed by disk diffusion method and minimum inhibitory concentrations of azoles, caspofungin, and amphotericin B against C. tropicalis were determined by broth microdilution. Polymorphism of erg11 gene associated with fluconazole resistance was carried out by polymerase chain reaction and sequencing. Multilocus sequence typing (MLST) was used for typing selected C. albicans isolates. Results: Overall, 196 Candida isolates were detected, mostly C. albicans (48%), followed by C. tropicalis (16%), C. parapsilosis (11%), C. glabrata (9%), C. orthopsilosis (6%) and to a lesser extent another eight species. High rates of resistance to fluconazole and voriconazole (18.8%) were observed in C. tropicalis with five isolates co-resistant to both agents. Y132F and S154F missense mutations in the ERG11 protein were associated with fluconazole-resistance in C. tropicalis (67.7%). Resistance to caspofungin was found in one isolate of C. albicans. MLST identified a polyclonal population of C. albicans with multiple diploid sequence types, and with few lineages showing potential nosocomial spread. Conclusions: Resistance to triazole agents should be considered in C. tropicalis infections in the studied hospitals, and surveillance measures taken to avoid Candida diffusion.

show abstract

Section: Discussionmentioning

confidence: 99%

“…In Vietnam, fungal opportunistic diseases are growing due to the increased number of immunocompromised patients [9,10]. Although physicians have dealt with Candida infections more frequently, there are limited systematic epidemiological data on Candida in the country [11][12][13], even less from Central Vietnam.…”

Section: Introductionmentioning

confidence: 99%

Azole non-susceptible C. tropicalis and polyclonal spread of C. albicans in Central Vietnam hospitals

Ngo

Santona

Fiamma

et al. 2023

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

Antifungal and antibiofilm activities of bee venom loaded on chitosan nanoparticles: a novel approach for combating fungal human pathogens

El-Didamony

Kalaba

El‐Fakharany

et al. 2022

World J Microbiol Biotechnol

View full text Add to dashboard Cite

The prevalence of opportunistic human fungal pathogens is increasing worldwide, and antimicrobial resistance is one of the greatest medical challenges the world faces. Therefore, this study aimed to develop a novel agent to control fungal pathogens. The honeybee products (honey, royal jelly, propolis, bee bread, and bee venom) were screened against unicellular fungal (UCF) pathogens (Cryptococcus neoformans, Kodamaea ohmeri, and Candida albicans) and the bee venom was only exhibited an inhibitory effect against them. The protein contents of crude bee venom were separated using the gel filtration technique into eight fractions which were visualized on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) to confirm the presence of five bands with molecular weights of 65, 43, 21, 15, and 3 KDa. Bee venom (BV) of Apis mellifera loaded chitosan nanoparticles were prepared by the ionotropic gelation method. The encapsulation efficiency%, average size, zeta potentials, and surface appearance by Transmission electron microscope (TEM) were evaluated for the prepared nanoparticles. The minimum inhibitory concentration (MIC) of crude BV and BV loaded chitosan nanoparticles (BV-CNPs) was evaluated against the offer mentioned UCF where the MIC values of crude BV were 6.25, 3.12 & 6.25 while MIC values in the case of BV-CNPs were decreased to 3.12, 3.12 & 1.56 mg/ml against C. neoformans, K. ohmeri and C. albicans, respectively. Also, the results showed that BV-CNPs suppressed the biofilm formation as well as yeast to hyphal transition formed by the examined UCF. These results revealed that BV-CNPs are a promising natural compound for fungal pathogens treatment. Graphical abstract

show abstract

Prevalence, species distribution, and risk factors of fungal colonization and infection in patients at a burn intensive care unit in Vietnam

Bang¹,

Xuan²,

Quang³

et al. 2020

CMM

View full text Add to dashboard Cite

Background and Purpose: Burn patients are at a higher risk of infections caused by different organisms. This study aimed to address the prevalence, causative species, and factors related to fungal colonization or infection in patients with acute severe injuries admitted to the intensive care unit (ICU) of a burn hospital in northern Vietnam. Materials and Methods: This prospective study was conducted on 400 patients in a burn ICU between 2017 and 2019. Clinical samples were weekly collected and screened for fungi, and relevant clinical information was obtained from medical records. Results: According to the results, 90% of the patients were colonized with fungi. Out of this group, 12.75% of the cases had invasive fungal infection (IFI). Eleven yeasts and six mold species were isolated from the patients, with the most common species being Candida tropicalis (45.56%) and C. albicans (41.94%). Among the eleven species causing fungal wound infection (FWI), the most common agents were Candida (66.7% of FWI patients) and Aspergillus (38.5%) species. Three Candida species isolated from blood were C. tropicalis (66.7%), C. albicans (20.0%), and C. parapsilosis (14.3%). No factors were found to expose the patients to a higher risk of fungal colonization. However, hyperglycemia, prolonged ICU stay, and heavy Candida species colonization were found to be independently predictive of IFI. Conclusion: Burn patients are at the risk of fungal infection with Candida species (especially C. tropicalis) and Aspergillus as the most frequently responsible agents. Continuous surveillance of fungi and appropriate management of pathophysiological consequences are essential to prevent fungal infection in burn patients.

show abstract

Prevalence of Candida bloodstream isolates from patients in two hospitals in Vietnam

Cited by 3 publications

References 28 publications

Azole non-susceptible C. tropicalis and polyclonal spread of C. albicans in Central Vietnam hospitals

Azole non-susceptible C. tropicalis and polyclonal spread of C. albicans in Central Vietnam hospitals

Antifungal and antibiofilm activities of bee venom loaded on chitosan nanoparticles: a novel approach for combating fungal human pathogens

Prevalence, species distribution, and risk factors of fungal colonization and infection in patients at a burn intensive care unit in Vietnam

Contact Info

Product

Resources

About