Prevalence of Campylobacter species in fecal samples of pigs and humans from Zuru Kebbi State, Nigeria

Gwimi, P. B.; Faleke, O. O.; Salihu, M. D.; Magaji, A. A.; Abubakar, Mustapha; Nwankwo, Innocent Okwundu; Ibitoye, E. B.

doi:10.14202/ijoh.2015.1-5

Cited by 23 publications

(33 citation statements)

References 10 publications

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“…Humans in the study area are at risk of gastroenteritis since the most common bacterial causes of infectious intestinal disease, C. coli and C. jejuni have been identified (Altekruse et al, 1999;Coker et al, 2002;Uaboi-Egbenni et al, 2008). The Campylobacter species prevalence rates in humans were in agreement with the rates recorded in the work of Gwimi et al (2015) in a neighboring state of Kebbi, Nigeria where C. coli was higher in occurrence than other species. However, the findings disagreed with studies that revealed C. jejuni as the most common species of Campylobacter in humans in Spain and Nigeria (Saenz et al, 2000;Ohanu & Offune 2009).…”

Section: Resultssupporting

confidence: 87%

Prevalence and molecular identification of Campylobacter species isolates from poultry and humans in Sokoto, Nigeria

Nwankwo¹,

Faleke²,

Salihu³

et al. 2017

Sokoto J. Vet. Sc.

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Prevalence and molecular identification of Campylobacter species isolates from poultry and humans were conducted using culture, biochemical reaction and Polymerase Chain Reaction (PCR) techniques. A total of 798 (506 poultry and 292 human) samples were identified biochemically, out of which 312(39.1%) were positive for Campylobacter species. Campylobacter jejuni, C. coli and C. lari had 38 (23.8%) out of 160, 63(39.4%) out of 160, 59(36.9%) out of 160 prevalence rates, respectively in humans while 29(19.1%) out of 152, 79(52.0%) out of 152 and 44(28.9%) out of 152 were the rates for the species in the same order in poultry. Campylobacter isolates were kept at -20 o C in 15% glycerol and 85% tryptone broth until used while some were identified 24hrs post isolation. Single and multiplex PCR were used to confirm the genus Campylobacter and three Campylobacter species, respectively. All the 130(100 stored and 30 fresh) isolates were members of the genus Campylobacter. The single PCR band view of stored isolates also revealed other bands in addition to 439 bp which is specific for the genus Campylobacter, while the fresh isolates had distinct bands at 439bp only. Multiplex PCR revealed 2(6.7%) out of 30 were positive for stored isolates out of 30, 1(50%) each for C. jejuni and C. Coli. However, 1 of the stored isolate was positive for both spp. On the other hand, 6(20.0%) of out 60 fresh isolates were positive, with 5(83.3%) and 1(16.7%) for C. jejuni and C. coli, respectively. The possibilities of improper identification using conventional method have been revealed in the study. PCR can identify Campylobacter species more accurately than biochemical method, though storage of isolates, integrity of extracted DNA and PCR conditions can affect result. However, the use of both methods should be encouraged in regular and effective surveillance of Campylobacter species in poultry and humans.

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Section: Resultssupporting

confidence: 87%

Prevalence and molecular identification of Campylobacter species isolates from poultry and humans in Sokoto, Nigeria

Nwankwo¹,

Faleke²,

Salihu³

et al. 2017

Sokoto J. Vet. Sc.

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“…The 17.3% prevalence obtained in our study is within the documented range of 20% in developing countries [13] [2]. This may be attributed to the design of this study which did not focus on children with acute diarrhoea and also a reflection of the infection sources which were mostly obtained from the outpatient department which is usually dominated by these age groups in the study hospital.…”

Section: Discussionsupporting

confidence: 72%

“…Multidrug resistance of Campylobacter species from patients. (24.5%) from human samples in Nigeria [2]. Campylobacter lari is known to infrequently cause human diseases compared to C. jejuni and C. coli.…”

Section: Discussionmentioning

confidence: 99%

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Antibiotic Resistance in Campylobacter Isolated from Patients with Gastroenteritis in a Teaching Hospital in Ghana

Karikari¹,

Obiri‐Danso²,

Frimpong³

et al. 2017

OJMM

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Campylobacter is a leading causal agent of bacterial enteritis worldwide, but its prevalence is not well documented in Ghanaian hospitals. This study isolated Campylobacter species from patients with enteritis or urinary tract infections attending Komfo Anokye Teaching Hospital and assessed the antibiogram profile of isolated species. Two hundred and two (202) in-patients and outpatients samples of all age groups diagnosed with enteritis or UTI infections were analyzed from May 2013 to August 2013. Campylobacter species were detected using selective agar (mCCDA) and confirmed on API Campy system (bioMérieux, France), with disk diffusion method determined the resistance profile of the species. Of the 128 enteritis and 74 UTI patients samples analyzed, 26 and 9 isolates were respectively confirmed as Campylobacter spp. giving a prevalence of 17.3% (35/202). Species identified were C. jejuni (40%), C. jejuni sub sp. doylei (2.8%), C. coli (37%) and C. lari (20%). Resistance was 92.3% -100% each to erythromycin and the β-lactams, 61.5% -86.7% to trimethoprim sulfamethoxazole, 92.3% -93.3% to tetracycline, 46.2% -80% to chloramphenicol, 0% -60% to aminoglycosides and 0% to imipenem. Multidrug resistance of 97.1% was detected among species. Empirical treatment of Campylobacter enteritis with erythromycin and other common and cheap drugs may result in treatment failure in the face of high level resistance observed among the Campylobacter species.

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“…This was supported by the findings of [10], who reported that C. coli were more prevalence than C. jejuni in Leipzig, Germany. This work also agrees with the work of [1] and [11] who also reported a higher prevalence of C. coli than C. jejuni. However, this observation was not in accordance with previous studies that Campylobacter jejuni is the most common thermotolerant Campylobacter species [23,25,30].…”

Section: Prevalence and Distribution Of Campylobacter Species In The supporting

confidence: 93%

Prevalence and Antibiotic Susceptibility Patterns of Campylobacter Species in Locally Pasteurized Milk Product (Nunu) Sold in Zaria Metropolis, Kaduna State, Nigeria

Ogbomon¹

2018

IJMB

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Abstracts: Contaminated milk and milk-products are regarded as vehicles for the transmission of Campylobacteriosis, infectious diarrhoea caused by Campylobacter but the prevalence of this bacterium in nunu had not been established. This study aimed at determining the prevalence of Campylobacter species in locally pasteurized milk product (nunu) sold in Zaria metropolis and establish the antibiotic resistance pattern of the isolates. A total of 180 nunu samples were collected and cultured for Campylobacter species using membrane filtration method, characterized using biochemical testing and API campy kits. Susceptibility of the isolates was carried out using the conventional agar diffusion method and the MAR indexing was calculated respectively. Out of the 180 nunu samples, 29 samples were positive for Campylobacter species giving a prevalence of 16.1%. Campylobacter coli were mostly isolated with isolation rate of 10.5% while C. jejuni were 5.6%. Imipenem were found to have 100% efficacy against all the Campylobacter species followed by Gentamicin (95%) while the isolates were resistant to Erythromycin (100%). Altogether, 39% of the Campylobacter species were resistant to three and more class of antibiotics and this is mostly implicated among strains of Campylobacter coli (47%) compared to C. jejuni (31%). The highest MAR index of 0.63 observed in this study is of public health importance. In this study, all the isolates had a MAR index greater than 0.2. Thus, Campylobacter contamination of the milk product is likely to be from a high risk source. With the increasing trend in Campylobacter resistance, it is therefore recommended that the use of antibiotic in animal food production and human therapy had to be controlled.

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Prevalence of Campylobacter species in fecal samples of pigs and humans from Zuru Kebbi State, Nigeria

Cited by 23 publications

References 10 publications

Prevalence and molecular identification of <i>Campylobacter</i> species isolates from poultry and humans in Sokoto, Nigeria

Prevalence and molecular identification of <i>Campylobacter</i> species isolates from poultry and humans in Sokoto, Nigeria

Antibiotic Resistance in <i>Campylobacter</i> Isolated from Patients with Gastroenteritis in a Teaching Hospital in Ghana

Prevalence and Antibiotic Susceptibility Patterns of <i>Campylobacter</i> Species in Locally Pasteurized Milk Product (Nunu) Sold in Zaria Metropolis, Kaduna State, Nigeria

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Prevalence of Campylobacter species in fecal samples of pigs and humans from Zuru Kebbi State, Nigeria

Cited by 23 publications

References 10 publications

Prevalence and molecular identification of <i>Campylobacter</i> species isolates from poultry and humans in Sokoto, Nigeria

Prevalence and molecular identification of <i>Campylobacter</i> species isolates from poultry and humans in Sokoto, Nigeria

Antibiotic Resistance in &lt;i&gt;Campylobacter&lt;/i&gt; Isolated from Patients with Gastroenteritis in a Teaching Hospital in Ghana

Prevalence and Antibiotic Susceptibility Patterns of &lt;i&gt;Campylobacter&lt;/i&gt; Species in Locally Pasteurized Milk Product (Nunu) Sold in Zaria Metropolis, Kaduna State, Nigeria

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Antibiotic Resistance in <i>Campylobacter</i> Isolated from Patients with Gastroenteritis in a Teaching Hospital in Ghana

Prevalence and Antibiotic Susceptibility Patterns of <i>Campylobacter</i> Species in Locally Pasteurized Milk Product (Nunu) Sold in Zaria Metropolis, Kaduna State, Nigeria