Prevalence of Campylobacter species in milk and milk products, their virulence gene profile and antibiogram

Modi, Shivani; Brahmbhatt, M. N.; Chatur, Yogesh A.; Nayak, J. B.

doi:10.14202/vetworld.2015.1-8

Cited by 46 publications

(49 citation statements)

References 50 publications

(42 reference statements)

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“…C. coli is frequently isolated from pork (QIN et al, 2011;GHIMIRE et al, 2014;WYSOK et al, 2015a;WYSOK et al, 2015b). Although, the lower rates were detected in raw milk and dairy products (WHYTE et al, 2004;HUSSAIN et al, 2007;BIANCHI et al, 2013;MODI et al, 2015;BERTASI et al, 2016;CHRISTIDIS et al, 2016) (2016) demonstrated a 27.3%, 20%, 17.2%, 13.4% prevalence of Campylobacter in milk samples, respectively. All this emphasizes the importance of raw milk and dairy products as a potential source of Campylobacter.…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial susceptibility and virulence-associated genes in Campylobacter isolates from milk and wastewater in Hatay, Turkey

Elmali

Can

2019

Cienc. Rural

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Campylobacter is one of the most common causes of bacterial foodborne diseases throughout the world. This study was conducted to determine the prevalence, antimicrobial resistance and virulence of Campylobacter isolates of raw cow’s milk and cattle slaughterhouse wastewater samples in Hatay, Turkey. A total of 114 raw milk and 78 wastewater samples were analyzed for the identification of C. jejuni, C. coli, and C. lari by multiplex PCR. The overall prevalence of Campylobacter was found to be 7.2%, of these isolates, 85.7% were identified as C. jejuni and 14.2% as C. coli, but C. lari was not detected in the study. The cdtA and cadF genes were present in 66.6% and 41.6% of C. jejuni isolates tested, respectively, but wlaN gene was not found in any of the isolates. Results of antimicrobial resistance analysis showed that 71.4% of the isolates were resistant to erythromycin, 64.2% to tetracycline, and 57.1% to ciprofloxacin. Overall, 8 of 14 Campylobacter isolates (57.1%) showed a multidrug resistance.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial susceptibility and virulence-associated genes in Campylobacter isolates from milk and wastewater in Hatay, Turkey

Elmali

Can

2019

Cienc. Rural

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“…The Campylobacteriaceae family is divided into four genera: Campylobacter , Arcobacter , Dehalospirilum , and Sulfurospirilum [ 26 ]. Campylobacter spp.…”

Section: Campylobacteriosismentioning

confidence: 99%

Campylobacteriosis, Salmonellosis, Yersiniosis, and Listeriosis as Zoonotic Foodborne Diseases: A Review

Chlebicz-Wójcik

Śliżewska

2018

IJERPH

346

227

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Zoonoses are diseases transmitted from animals to humans, posing a great threat to the health and life of people all over the world. According to WHO estimations, 600 million cases of diseases caused by contaminated food were noted in 2010, including almost 350 million caused by pathogenic bacteria. Campylobacter, Salmonella, as well as Yersinia enterocolitica and Listeria monocytogenes may dwell in livestock (poultry, cattle, and swine) but are also found in wild animals, pets, fish, and rodents. Animals, often being asymptomatic carriers of pathogens, excrete them with faeces, thus delivering them to the environment. Therefore, pathogens may invade new individuals, as well as reside on vegetables and fruits. Pathogenic bacteria also penetrate food production areas and may remain there in the form of a biofilm covering the surfaces of machines and equipment. A common occurrence of microbes in food products, as well as their improper or careless processing, leads to common poisonings. Symptoms of foodborne infections may be mild, sometimes flu-like, but they also may be accompanied by severe complications, some even fatal. The aim of the paper is to summarize and provide information on campylobacteriosis, salmonellosis, yersiniosis, and listeriosis and the aetiological factors of those diseases, along with the general characteristics of pathogens, virulence factors, and reservoirs.

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“…The identified Campylobacter species were further screened for the presence of invasion genes (ciaB and iam), adherence genes (flaA and cadF), a toxin gene (cdtB) and flagella synthesis, and a modification gene (flgR). The primer sets for the detection of cdtB, flaA and cadF genes were used as reported by Modi et al [24], iam gene [42], ciaB gene [43] and flgR) gene [44].…”

Section: Molecular Detection Of Virulence Genesmentioning

confidence: 99%

Campylobacteriosis Agents in Meat Carcasses Collected from Two District Municipalities in the Eastern Cape Province, South Africa

Igwaran

Okoh

2020

Foods

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Raw meats are sometimes contaminated with Campylobacter species from animal faeces, and meats have repeatedly been implicated in foodborne infections. This study evaluated the prevalence, virulence genes, antimicrobial susceptibility patterns, and resistance gene determinants in Campylobacter species isolated from retailed meat carcasses. A total of 248 raw meat samples were collected from butcheries, supermarkets, and open markets; processed for enrichment in Bolton broth; and incubated at 42 °C for 48 h in 10% CO2. Thereafter, the broths were streaked on modified charcoal cefoperazone deoxycholate agar (mCCDA) plates and incubated at the same conditions and for the same amount of time. After incubation, colonies were isolated and confirmed by Polymerase chain reaction using specific oligonucleotide sequences used for the identification of the genus Campylobacter, species, and their virulence markers. The patterns of antimicrobial resistance profiles of the identified isolates were studied by disk diffusion method against 12 antibiotics, and relevant resistance genes were assessed by PCR. From culture, 845 presumptive Campylobacter isolates were obtained, of which 240 (28.4%) were identified as genus Campylobacter. These were then characterised into four species, of which C. coli had the highest prevalence rate (22.08%), followed by C. jejuni (16.66%) and C. fetus (3.73%). The virulence genes detected included iam (43.14%), cadF (37.25%), cdtB (23.53%), flgR (18.63%), and flaA (1.96%), and some of the isolates co-harboured two to four virulence genes. Of the 12 antibiotics tested, the highest phenotypic resistance displayed by Campylobacter isolates was against clindamycin (100%), and the lowest level of resistance was observed against imipenem (23.33%). The frequency of resistance genes detected included catll (91.78%), tetA (68.82%), gyra (61.76%), ampC (55%), aac(3)-IIa (aacC2)a (40.98%), tetM (38.71%), ermB (18.29%), tetB (12.90%), and tetK (2.15%). There is a high incidence of Campylobacter species in meat carcasses, suggesting these to be a reservoir of campylobacteriosis agents in this community, and as such, consumption of undercooked meats in this community is a potential health risk to consumers.

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Prevalence of Campylobacter species in milk and milk products, their virulence gene profile and antibiogram

Cited by 46 publications

References 50 publications

Antimicrobial susceptibility and virulence-associated genes in Campylobacter isolates from milk and wastewater in Hatay, Turkey

Antimicrobial susceptibility and virulence-associated genes in Campylobacter isolates from milk and wastewater in Hatay, Turkey

Campylobacteriosis, Salmonellosis, Yersiniosis, and Listeriosis as Zoonotic Foodborne Diseases: A Review

Campylobacteriosis Agents in Meat Carcasses Collected from Two District Municipalities in the Eastern Cape Province, South Africa

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