Prevalence of Borrelia miyamotoi in Ixodes persulcatus in Irkutsk City and its neighboring territories, Russia

Khasnatinov, M. A.; Данчинова, Г. А.; Takano, Ai; Kawabata, Hiroki; Ohashi, Norio; Masuzawa, Toshiyuki

doi:10.1016/j.ttbdis.2015.12.016

Cited by 14 publications

(12 citation statements)

References 16 publications

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“…Subsequently it was discovered to be occurring sympatrically with B. burgdorferi sensu lato in several Ixodes species that also transmit Lyme disease spirochetes. These included Ixodes persulcatus in Eurasia [2][3][4][5][6][7], I. scapularis [8][9][10][11] and I. pacificus [12][13][14][15] in North America, and I. ricinus in Europe [16][17][18][19][20]. The prevalence of B. miyamotoi in ticks was found to be usually lower than that of B. burgdorferi s.l.…”

Section: Introductionmentioning

confidence: 99%

“…The prevalence of B. miyamotoi in ticks was found to be usually lower than that of B. burgdorferi s.l. although prevalences of~15% have been reported in some regions [3,7,10,16,17,21,22]. Rodents have been implicated as reservoir hosts for B. miyamotoi [23,24], but transovarial transmission is also known to occur [25,26] and may contribute to the persistence of this Borrelia in nature.…”

Section: Introductionmentioning

confidence: 99%

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Whole genome sequencing of Borrelia miyamotoi isolate Izh-4: reference for a complex bacterial genome

Kuleshov

Margos²,

Fingerle³

et al. 2020

BMC Genomics

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Background: The genus Borrelia comprises spirochaetal bacteria maintained in natural transmission cycles by tick vectors and vertebrate reservoir hosts. The main groups are represented by a species complex including the causative agents of Lyme borreliosis and relapsing fever group Borrelia. Borrelia miyamotoi belongs to the relapsing fever group of spirochetes and forms distinct populations in North America, Asia, and Europe. As all Borrelia species B. miyamotoi possess an unusual and complex genome consisting of a linear chromosome and a number of linear and circular plasmids. The species is considered an emerging human pathogen and an increasing number of human cases are being described in the Northern hemisphere. The aim of this study was to produce a high quality reference genome that will facilitate future studies into genetic differences between different populations and the genome plasticity of B. miyamotoi. Results: We used multiple available sequencing methods, including Pacific Bioscience single-molecule real-time technology (SMRT) and Oxford Nanopore technology (ONT) supplemented with highly accurate Illumina sequences, to explore the suitability for whole genome assembly of the Russian B. miyamotoi isolate, Izh-4. Plasmids were typed according to their potential plasmid partitioning genes (PF32, 49, 50, 57/62). Comparing and combining results of both long-read (SMRT and ONT) and short-read methods (Illumina), we determined that the genome of the isolate Izh-4 consisted of one linear chromosome, 12 linear and two circular plasmids. Whilst the majority of plasmids had corresponding contigs in the Asian B. miyamotoi isolate FR64b, there were only four that matched plasmids of the North American isolate CT13-2396, indicating differences between B. miyamotoi populations. Several plasmids, e.g. lp41, lp29, lp23, and lp24, were found to carry variable major proteins. Amongst those were variable large proteins (Vlp) subtype Vlp-α, Vlp-γ, Vlp-δ and also Vlp-β. Phylogenetic analysis of common plasmids types showed the uniqueness in Russian/Asian isolates of B. miyamotoi compared to other isolates. Conclusions: We here describe the genome of a Russian B. miyamotoi clinical isolate, providing a solid basis for future comparative genomics of B. miyamotoi isolates. This will be a great impetus for further basic, molecular and epidemiological research on this emerging tick-borne pathogen.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Whole genome sequencing of Borrelia miyamotoi isolate Izh-4: reference for a complex bacterial genome

Kuleshov

Margos²,

Fingerle³

et al. 2020

BMC Genomics

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“…On the other hand, on Hokkaido Island, Japan, where, depending on the tick species, 2% (71/3,532 I. persulcatus ) and 4.3% (5/117 I. pavlovskyi ) of ticks were infected, human cases did occur [4]. Human cases were also observed in the Irkutsk region, Russia, where an overall 2.9% prevalence of tick infection has been estimated [21] (Table 1). In Yekaterinburg and Izhervsk, where clinical cases were first described, tick ( I. persulcatus ) infection prevalence rates of 0.9% (4/442) and 6.3% (25/394) were respectively found.…”

Section: Resultsmentioning

confidence: 99%

“…In the Netherlands, it was estimated that tick bites amount to 71,980 per million inhabitants (2007) [60]. In the Irkutsk region in Russia 2014, tick bites were estimated at 12,500 per million people [21]. The prevalence of infection among ticks ranges from 0.5 to 6% in many regions of the northern hemisphere.…”

Section: Resultsmentioning

confidence: 99%

A new Borrelia on the block: Borrelia miyamotoi – a human health risk?

et al. 2019

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Background Borrelia miyamotoi clusters phylogenetically among relapsing fever borreliae, but is transmitted by hard ticks. Recent recognition as a human pathogen has intensified research into its ecology and pathogenic potential. Aims We aimed to provide a timely critical integrative evaluation of our knowledge on B. miyamotoi , to assess its public health relevance and guide future research. Methods This narrative review used peer-reviewed literature in English from January 1994 to December 2018. Results Borrelia miyamotoi occurs in the world’s northern hemisphere where it co-circulates with B. burgdorferi sensu lato, which causes Lyme disease. The two borreliae have overlapping vertebrate and tick hosts. While ticks serve as vectors for both species, they are also reservoirs for B. miyamotoi . Three B. miyamotoi genotypes are described, but further diversity is being recognised. The lack of sufficient cultivable isolates and vertebrate models compromise investigation of human infection and its consequences. Our understanding mainly originates from limited case series. In these, human infections mostly present as influenza-like illness, with relapsing fever in sporadic cases and neurological disease reported in immunocompromised patients. Unspecific clinical presentation, also occasionally resulting from Lyme- or other co-infections, complicates diagnosis, likely contributing to under-reporting. Diagnostics mainly employ PCR and serology. Borrelia miyamotoi infections are treated with antimicrobials according to regimes used for Lyme disease. Conclusions With co-infection of tick-borne pathogens being commonplace, diagnostic improvements remain important. Developing in vivo models might allow more insight into human pathogenesis. Continued ecological and human case studies are key to better epidemiological understanding, guiding intervention strategies.

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“…(hard ticks; Fukunaga et al., ; Scoles et al., ). B. miyamotoi DNA has been identified in the four main species of Ixodes that also carry B. burgdorferi sensu lato (i.e., I. persulcatus, I. ricinus , I. pacificus , and I. scapularis ; Fukunaga et al., ; Scoles et al., ; Richter et al., ; Barbour et al., ; Mays et al., ; Salkeld et al., ; Cochez et al., ; Eshoo et al., ; Khasnatinov et al., ).…”

Section: Introductionmentioning

confidence: 99%

Laboratory Cultivation and Maintenance of Borrelia miyamotoi

Stone

Brissette

2016

CP Microbiology

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Borrelia miyamotoi is a relapsing fever tick-borne pathogen found in Ixodes spp. (hard) ticks. In vitro culturing has proven difficult despite initial reports of cultures maintained in Barbour-Stoenner-Kelly-II (BSK-II) medium. The ability to culture in vitro opens many avenues for investigating the genetics and physiology of bacterial species. This unit describes methods for the maintenance and cultivation of B. miyamotoi in liquid medium. © 2016 by John Wiley & Sons, Inc.

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Prevalence of Borrelia miyamotoi in Ixodes persulcatus in Irkutsk City and its neighboring territories, Russia

Cited by 14 publications

References 16 publications

Whole genome sequencing of Borrelia miyamotoi isolate Izh-4: reference for a complex bacterial genome

Whole genome sequencing of Borrelia miyamotoi isolate Izh-4: reference for a complex bacterial genome

A new Borrelia on the block: Borrelia miyamotoi – a human health risk?

Laboratory Cultivation and Maintenance of Borrelia miyamotoi

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