Prevalence of blood-borne viral infections (cytomegalovirus, human herpesvirus-6, human herpesvirus-7, human herpesvirus-8, human T-cell lymphotropic virus-I/II, human retrovirus-5) among blood donors in Latvia

Kozireva, Svetlana; Nemceva, G; Danilane, I; Павлова, О Г; Blomberg, Jonas; Murovska, Modra

doi:10.1007/s002770100359

Cited by 24 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also possible that individuals infected with colobus CMV may have remained unidentified because the virus was not shed in saliva and feces or because the applied PCRs did not possess maximum sensitivity for detection of CMVs. Despite this, the 2.6 % HCMV prevalence determined here in a collection of oral and fecal swabs is well in accord with previous PCR-based studies on healthy subjects reporting HCMV detection rates in blood of 0–8 % of children and adults from Uganda, Germany, Latvia, Australia and Japan [ 39 – 43 ] (studies based on fecal samples were to our knowledge not reported). In addition, sequences of other viruses have been detected previously in the present sample set [ 33 ] indicating absence of PCR inhibitors, and the use of colobus-specific PCR able to identify colobus CMV in control colobus monkey samples indicates this PCR is of sufficient sensitivity to detect colobus CMV present at levels found within its natural host.…”

Section: Resultssupporting

confidence: 91%

Genetic identification of cytomegaloviruses in a rural population of Côte d’Ivoire

Anoh

Akoua-Koffi

Couacy‐Hymann

et al. 2015

Virol J

View full text Add to dashboard Cite

BackgroundCytomegaloviruses (CMVs) are herpesviruses that infect many mammalian species, including humans. Infection generally passes undetected, but the virus can cause serious disease in individuals with impaired immune function. Human CMV (HCMV) is circulating with high seroprevalence (60–100 %) on all continents. However, little information is available on HCMV genoprevalence and genetic diversity in subsaharan Africa, especially in rural areas of West Africa that are at high risk of human-to-human HCMV transmission. In addition, there is a potential for zoonotic spillover of pathogens through bushmeat hunting and handling in these areas as shown for various retroviruses. Although HCMV and nonhuman CMVs are regarded as species-specific, potential human infection with CMVs of non-human primate (NHP) origin, shown to circulate in the local NHP population, has not been studied.FindingsAnalysis of 657 human oral swabs and fecal samples collected from 518 individuals living in 8 villages of Côte d’Ivoire with generic PCR for identification of human and NHP CMVs revealed shedding of HCMV in 2.5 % of the individuals. Determination of glycoprotein B sequences showed identity with strains Towne, AD169 and Toledo, respectively. NHP CMV sequences were not detected.ConclusionsHCMV is actively circulating in a proportion of the rural Côte d’Ivoire human population with circulating strains being closely related to those previously identified in non-African countries. The lack of NHP CMVs in human populations in an environment conducive to cross-species infection supports zoonotic transmission of CMVs to humans being at most a rare event.

show abstract

Section: Resultssupporting

confidence: 91%

Genetic identification of cytomegaloviruses in a rural population of Côte d’Ivoire

Anoh

Akoua-Koffi

Couacy‐Hymann

et al. 2015

Virol J

View full text Add to dashboard Cite

show abstract

“…The active single HHV-7 and B19 infection, but not active single HHV-6 or active concurrent infection, has been previously detected in Latvian blood donors [33]. The active HHV-6 infection was confirmed by the detection of viral sequence in plasma DNA samples and a concomitant increase of HHV-6 load in PBLs.…”

Section: Discussionmentioning

confidence: 63%

Association of Active Human Herpesvirus-6, -7 and Parvovirus B19 Infection with Clinical Outcomes in Patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome

Chapenko

Krūmiņa

Logina

et al. 2012

Advances in Virology

View full text Add to dashboard Cite

Frequency of active human herpesvirus-6, -7 (HHV-6, HHV-7) and parvovirus B19 (B19) infection/coinfection and its association with clinical course of ME/CFS was evaluated. 108 ME/CFS patients and 90 practically healthy persons were enrolled in the study. Viral genomic sequences were detected by PCR, virus-specific antibodies and cytokine levels—by ELISA, HHV-6 variants—by restriction analysis. Active viral infection including concurrent infection was found in 64.8% (70/108) of patients and in 13.3% (12/90) of practically healthy persons. Increase in peripheral blood leukocyte DNA HHV-6 load as well as in proinflammatory cytokines' levels was detected in patients during active viral infection. Definite relationship was observed between active betaherpesvirus infection and subfebrility, lymphadenopathy and malaise after exertion, and between active B19 infection and multijoint pain. Neuropsychological disturbances were detected in all patients. The manifestation of symptoms was of more frequent occurrence in patients with concurrent infection. The high rate of active HHV-6, HHV-7 and B19 infection/coinfection with the simultaneous increase in plasma proinflammatory cytokines' level as well as the association between active viral infection and distinctive types of clinical symptoms shows necessity of simultaneous study of these viral infections for identification of possible subsets of ME/CFS.

show abstract

Epidemiology of Epstein–Barr virus, cytomegalovirus, and kaposi's sarcoma-associated herpesvirus infections in peripheral blood leukocytes revealed by a multiplex PCR assay

et al. 2006

View full text Add to dashboard Cite

A multiplex polymerase chain reaction (PCR) has been developed for the simultaneous detection of Epstein-Barr virus (EBV), cytomegalovirus (CMV), and Kaposi's sarcoma-associated herpesvirus (KSHV) in a clinical sample. Primers of multiplex PCR were designed to amplify specific regions of the EBV EBNA1, CMV IE2, and KSHV LANA genes. This multiplex PCR assay was found to have detection sensitivities of 1-10 copies of purified viral DNA cloned into the plasmid. To assess diagnostic and pre-clinical applications with this method, we utilized KSHV-positive primary effusion lymphoma (PEL) cells, EBV-positive Burkitt's lymphoma cells, CMV-infected fibroblast cells, and clinically prepared peripheral blood leukocytes (PBLs) that had been infected with viruses. We found that this multiplex PCR assay has high sensitivity and specificity for simultaneous detection of EBV, CMV, and KSHV genomes in a single amplification from a clinical material. Using this multiplex PCR assay, we investigated the prevalence of EBV, CMV, and KSHV in PBL samples from normal Japanese randomly selected. KSHV, EBV, and CMV genomes were detected in samples from 2 (0.2%), 377 (39.5%), and 27 (2.8%) of the 953 blood donors, respectively. Interestingly, both EBV and CMV genomes were detected in samples from all KSHV-positive donors.

show abstract

Prevalence of blood-borne viral infections (cytomegalovirus, human herpesvirus-6, human herpesvirus-7, human herpesvirus-8, human T-cell lymphotropic virus-I/II, human retrovirus-5) among blood donors in Latvia

Cited by 24 publications

References 41 publications

Genetic identification of cytomegaloviruses in a rural population of Côte d’Ivoire

Genetic identification of cytomegaloviruses in a rural population of Côte d’Ivoire

Association of Active Human Herpesvirus-6, -7 and Parvovirus B19 Infection with Clinical Outcomes in Patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome

Epidemiology of Epstein–Barr virus, cytomegalovirus, and kaposi's sarcoma-associated herpesvirus infections in peripheral blood leukocytes revealed by a multiplex PCR assay

Contact Info

Product

Resources

About