Prevalence of bacteria resistant to antibiotics and/or biocides on meat processing plant surfaces throughout meat chain production

Lerma, Leyre Lavilla; Benomar, Nabil; Gálvez, Antonio; Abriouel, Hikmate

doi:10.1016/j.ijfoodmicro.2012.11.028

Cited by 42 publications

(36 citation statements)

References 55 publications

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“…Long‐time extensive and inappropriate use of antibiotics for growth promotion or as therapeutic and preventive treatments in food animals may generate selective pressure for emergence of multiple drug resistant (MDR) bacteria (Barton ). The prevalence of MDR bacteria in farm, slaughterhouse environments, and retail foods have been reported in several studies (Lavilla Lerma and others ; Novais and others ; Casella and others ). Microorganisms, including pathogens and commensal bacteria present in animal foods and their processing environment may cause a great challenge for human health in terms of their role as a potential reservoir of antibiotic resistance genes (ARGs) and potential pathogenic power, which may transmit to humans through the food chain (Wang and others ).…”

Section: Introductionmentioning

confidence: 79%

Isolation and Identification of Aerobic Bacteria Carrying Tetracycline and Sulfonamide Resistance Genes Obtained from a Meat Processing Plant

Ye²,

Zhang

et al. 2016

Journal of Food Science

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Microbial contamination in food-processing plants can play a fundamental role in food quality and safety. The purpose of this study was to investigate aerobic bacteria carrying tetracycline and sulfonamide resistance genes from a meat processing plant as possible sources of meat contamination. One hundred swab samples from surfaces of conveyor belts, meat slicers, meat knives, benches, plastic trays, gloves, and aprons were analyzed. A total of 168 isolates belonging to 10 genera were obtained, including Pseudomonas sp. (n = 35), Acinetobacter sp. (n = 30), Aeromonas sp. (n = 20), Myroides sp. (n = 15), Serratia sp. (n = 15), Staphylococcus sp. (n = 14), Enterobacter sp. (n = 11), Escherichia coli (n = 10), Lactococcus sp. (n = 10), and Klebsiella sp. (n = 8). Of the 168 isolates investigated, 60.7% showed resistance to tetracycline and 57.7% to trimethoprim/sulfamethoxazole. The tetracycline resistance genes tetL, tetA, tetB, tetC, tetE, tetM, tetS, tetK, and tetX were found in the frequency of 7.7%, 6.0%, 4.8%, 4.8%, 3.6%, 3.6%, 3.6%, 1.2%, and 0.6%, respectively. Sulfonamide resistance genes sul1 and sul2 were observed in the frequency of 17.9% and 38.1%, respectively. The tetracycline resistance genes tetX was first found in Myroides sp. This investigation demonstrated that food contact surfaces in a meat processing plant may be sources of contamination of aerobic bacteria carrying tetracycline and sulfonamide antibiotic resistance genes.

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Section: Introductionmentioning

confidence: 79%

Isolation and Identification of Aerobic Bacteria Carrying Tetracycline and Sulfonamide Resistance Genes Obtained from a Meat Processing Plant

Ye²,

Zhang

et al. 2016

Journal of Food Science

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“…isolated from slaughterhouse surfaces and meat products. A collection of 122 isolates of antibiotic-resistant pseudomonads (85 and 37 mesophilic and psychrotrophic isolates, respectively) isolated by Lavilla Lerma et al (34) were reduced to 52 (38 mesophilic and 14 psychrotrophic) strains by ERIC-PCR analysis, since isolates with identical ERIC-PCR patterns were considered the same strain (see Fig. S1 in the supplemental material).…”

Section: Resultsmentioning

confidence: 99%

“…This study involved the analysis of phenotypic and genotypic antibiotic resistance profiles of 122 mesophilic and psychrotrophic pseudomonads isolated from slaughterhouse surfaces throughout the chain of meat production and also from the end products. Mesophilic (growth at 30°C for 72 h) and psychrotrophic (growth at 7°C for 10 days) pseudomonads were isolated as described by Lavilla Lerma et al (34) in King agar and tryptone soya agar, respectively. Furthermore, we evaluated the relationship between environmental pseudomonads and the end products with the aim of elucidating if they share an antibiotic resistome.…”

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confidence: 99%

Antibiotic Multiresistance Analysis of Mesophilic and Psychrotrophic Pseudomonas spp. Isolated from Goat and Lamb Slaughterhouse Surfaces throughout the Meat Production Process

Lerma

Benomar

Muñoz

et al. 2014

Appl Environ Microbiol

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The aim of this study was to investigate the phenotypic and genotypic antibiotic resistance profiles of pseudomonads isolated from surfaces of a goat and lamb slaughterhouse, which were representative of areas that are possible sources of meat contamination. Mesophilic (85 isolates) and psychrotrophic (37 isolates) pseudomonads identified at the species level generally were resistant to sulfamethoxazole, erythromycin, amoxicillin, ampicillin, chloramphenicol, trimethoprim, rifampin, and ceftazidime (especially mesophiles), as well as colistin and tetracycline (especially psychrotrophes). However, they generally were sensitive to ciprofloxacin, gentamicin, imipenem, and kanamycin regardless of species identity. Worryingly, in the present study, we found multidrug resistance (MDR) to up to 13 antibiotics, which was related to intrinsic and acquired resistance mechanisms. Furthermore, a link between various antimicrobial resistance genes was shown for beta-lactams and tetracycline, trimethoprim, and sulfonamides. The distribution and resistomebased analysis of MDR pseudomonads in different slaughterhouse zones indicated that the main sources of the identical or related pseudomonad strains were the animals (feet and wool) and the slaughterhouse environment, being disseminated from the beginning, or entrance environment, to the environment of the finished meat products. Those facts must be taken into consideration to avoid cross-contamination with the subsequent flow of mobile resistance determinants throughout all slaughterhouse zones and then to humans and the environment by the application of adequate practices of hygiene and disinfection measures, including those for animal wool and feet and also the entrance environment.

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“…Different processes in slaughterhouses like evisceration can contaminate carcasses and equipment with gut bacteria (Lavilla Lerma et al . ). The most commonly detected fecal coliforms in slaughterhouses are: Enterobacter , Citrobacter and Klebsiella (Schlegelova et al .…”

Section: Introductionmentioning

confidence: 97%

Evaluation of Bacterial Contamination Sources in Meat Production Line

Bakhtiary

Sayevand

Remely

et al. 2016

Journal of Food Quality

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Bacterial contamination during slaughtering process is a safety problem and concern for shelf life in meat production. We compare two different slaughter lines, sheep and cattle samples from Iranian slaughterhouse using PCR‐denaturing gradient gel electrophoresis (DGGE) analysis for evaluation the sources of bacterial contaminations. Bacterial diversity was significantly different between and within cattle and sheep slaughter lines. Samples are contaminated most frequently with Salmonella enterica (28% and 30%), Enterococcus fecalis (18% and 20%), Escherichia coli (13% and 14%) and Pseudomonas fluorescens (8% and 6%), either on equipment surfaces after general cleaning and sanitizing treatments (56%). Biodiversity of carcasses samples is increased during skinning and evisceration process and may indicate similar sources of contamination from hides and rectums of the animal to carcasses and environment. Practical Applications 16S rDNA based denaturing gradient gel electrophoresis‐PCR is a method for assessment of microbial diversity, source of contaminations and changes of microbial population in slaughterhouses.

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Prevalence of bacteria resistant to antibiotics and/or biocides on meat processing plant surfaces throughout meat chain production

Cited by 42 publications

References 55 publications

Isolation and Identification of Aerobic Bacteria Carrying Tetracycline and Sulfonamide Resistance Genes Obtained from a Meat Processing Plant

Isolation and Identification of Aerobic Bacteria Carrying Tetracycline and Sulfonamide Resistance Genes Obtained from a Meat Processing Plant

Antibiotic Multiresistance Analysis of Mesophilic and Psychrotrophic Pseudomonas spp. Isolated from Goat and Lamb Slaughterhouse Surfaces throughout the Meat Production Process

Evaluation of Bacterial Contamination Sources in Meat Production Line

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