Prevalence of antimicrobial‐resistant Escherichia coli in dogs in a cross‐sectional, community‐based study

Abstract

The prevalence of carriage of antimicrobial-resistant (AMR) and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli was determined in 183 healthy dogs from a semi-rural community in Cheshire. Isolates were tested against a panel of antimicrobials and by PCR to detect resistance genes. In the suspected ESBL-producing isolates, the presence of bla(SHV), bla(TEM), bla(CTX-M) and bla(AmpC) genes was determined by PCR and sequencing. A total of 53 (29 per cent, 95 per cent confidence interval [CI] 22.4 to 35.5 per cent) dogs carried at least one AMR E coli isolate. Twenty-four per cent (95 per cent CI 17.9 to 30.2 per cent) of dogs carried isolates resistant to ampicillin, 19.7 per cent (95 per cent CI 13.9 to 25.4 per cent) to tetracycline and 16.9 per cent (95 per cent CI 11.5 to 22.4 per cent) to trimethoprim. A bla(TEM) gene was detected in 39 of 54 ampicillin-resistant isolates, a tet(B) gene in 12 of 45 tetracycline-resistant isolates, and a dfr gene in 22 of 33 trimethoprim-resistant isolates. Multidrug-resistant isolates were demonstrated in 15 per cent (28 of 183; 95 per cent CI 10.1 to 20.5 per cent) of dogs. Nine suspected ESBL-producing E coli were isolated, of which only one was confirmed by double disc diffusion testing. Two of these isolates carried the bla(TEM-1) gene and seven carried the bla(CMY-2) gene.

Antimicrobial resistance, virulence genes and PFGE-profiling of Escherichia coli isolates from South Korean cattle farms

“…Only bla TEM was detected as a β-lactamase gene in this study, which agreed with previous reports (Karczmarczyk et al, 2011b;Wedley et al, 2011). The prevalence of genes encoding amoxicillin-and ampicillin-resistance was lower than those of the phenotype to the antimicrobials in our study.…”

High Prevalence of bla CTX-M-1 /IncI1/ST3 and bla CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

“…The emergence of this gene, which was pointed out in humans and animals in North and Central America in S. enterica and E. coli isolates (29), was also recently described in dogs, in the United Kingdom, the United States, and Greece (30)(31)(32). However, the proportion reported here (6.5%, ca.…”

Faecal Escherichia coli isolates from healthy dogs harbour CTX-M-15 and CMY-2 β-lactamases

“…CMY-type β-lactamase producing E. coli have been detected in 1-39% of healthy dogs in different parts of the world (Carattoli et al, 2005;Wedley et al, 2011;Tamang et al, 2012;Belas et al, 2014;Okubo et al, 2014). Identical ESBL producing strains of E. coli have been identified in dogs and their owners, suggesting cross-species transfer of faecal E. coli (Albrechtova et al, 2012).…”