Prevalence of antimicrobial resistance and virulence genes in Klebsiella pneumoniae and Congenetic Raoultella Isolates from captive giant pandas

Li, Yan; Sun, Yansong; Sun, Shi-Wen; Liu, Bing; Jiang, Bowen; Feng, Na; Li, Jun; Ji, Xue

doi:10.1371/journal.pone.0283738

Cited by 3 publications

(5 citation statements)

References 55 publications

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“…The dominant resistance genes in the study were derived from Pseudomonadota and predominantly annotated to Enterobacteriaceae, both of which were significantly higher in the bamboo leaf group. It is worth noting that many pathogenic bacteria, such as Escherichia, Klebsiella, Enterobacter, and Raoultella, belong to this taxonomic group [11,55,56], posing a serious threat to the survival of giant pandas. Klebsiella and Enterobacter can cause a wide range of infections [55], and their antibiotic resistance has been a major concern [56].…”

Section: Discussionmentioning

confidence: 99%

“…Frequent transfers of giant pandas to other enclosures may lead to the spread of antimicrobial drug resistance [1]. For example, multidrug-resistant Klebsiella pneumoniae presents a serious menace to the health of giant pandas and potential risks to wildlife center visitors and giant panda caretakers [11]. Comparatively, the microbiota of captive giant pandas exhibits a higher prevalence of both virulence genes and antimicrobial resistance genes (ARGs) in contrast to their counterparts in the wild [12].…”

Section: Introductionmentioning

confidence: 99%

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The Impact of Bamboo Consumption on the Spread of Antibiotic Resistance Genes in Giant Pandas

Yan,

He,

Ayala

et al. 2023

Veterinary Sciences

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The spread of antibiotic resistance genes (ARGs) in the environment exacerbates the contamination of these genes; therefore, the role plants play in the transmission of resistance genes in the food chain requires further research. Giant pandas consume different bamboo parts at different times, which provides the possibility of investigating how a single food source can affect the variation in the spread of ARGs. In this study, metagenomic analysis and the Comprehensive Antibiotic Resistance Database (CARD) database were used to annotate ARGs and the differences in gut microbiota ARGs during the consumption of bamboo shoots, leaves, and culms by captive giant pandas. These ARGs were then compared to investigate the impact of bamboo part consumption on the spread of ARGs. The results showed that the number of ARGs in the gut microbiota of the subjects was highest during the consumption of bamboo leaves, while the variety of ARGs was highest during the consumption of shoots. Escherichia coli, which poses a higher risk of ARG dissemination, was significantly higher in the leaf group, while Klebsiella, Enterobacter, and Raoultella were significantly higher in the shoot group. The ARG risk brought by bamboo shoots and leaves may originate from soil and environmental pollution. It is recommended to handle the feces of giant pandas properly and regularly monitor the antimicrobial and virulence genes in their gut microbiota to mitigate the threat of antibiotic resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Impact of Bamboo Consumption on the Spread of Antibiotic Resistance Genes in Giant Pandas

Yan,

He,

Ayala

et al. 2023

Veterinary Sciences

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“…Furthermore, overuse of antibiotics can enhance the prevalence of drug resistance genes, resulting in poorer treatment outcomes. Antibiotic-Resistant Escherichia coli and multidrug-resistant Klebsiella pneumoniae and Raoultella have been screened from the intestinal microbiota of giant pandas (Fan et al, 2022;Shahi et al, 2023). Therefore, it is particularly important to find a more effective way to treat intestinal diseases.…”

Section: Introductionmentioning

confidence: 99%

Clostridium butyricum isolated from giant panda can attenuate dextran sodium sulfate-induced colitis in mice

Yu,

Xie,

Guo

et al. 2024

Front. Microbiol.

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ObjectiveProbiotics are beneficial to the intestinal barrier, but few studies have investigated probiotics from giant pandas. This study aims to explore the preventive effects of giant panda-derived Clostridium butyricum on dextran sodium sulfate (DSS)-induced colitis in mice.MethodsClostridium butyricum was administered to mice 14 days before administering DSS treatment to induce enteritis.ResultsClostridium butyricum B14 could more effectively prevent colitis in mice than C. butyricum B13. C. butyricum B14 protected the mouse colon by decreasing the histology index and serum interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) levels, which improved intestinal inflammation-related symptoms. In addition, the treatment led to the regulation of the expression of Tifa, Igkv12-89, and Nr1d1, which in turn inhibited immune pathways. The expression of Muc4, Lama3, Cldn4, Cldn3, Ocln, Zo1, Zo2, and Snai is related the intestinal mucosal barrier. 16S sequencing shows that the C. butyricum B14 significantly increased the abundance of certain intestinal probiotics. Overall, C. butyricum B14 exerted a preventive effect on colitis in mice by inhibiting immune responses, enhancing the intestinal barrier and increasing the abundance of probiotic species. Thus, C. butyricum B14 administration helps regulate the balance of the intestinal microecology. It can suppress immune pathways and enhance barrier-protective proteins.

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“…Klebsiella pneumoniae can be found ubiquitously in natural superficial waters and soils [ 1 ] and colonize in the gastrointestinal tracts of humans and animals [ 2 – 4 ]. K .…”

Section: Introductionmentioning

confidence: 99%

“…Klebsiella pneumoniae can be found ubiquitously in natural superficial waters and soils [1] and colonize in the gastrointestinal tracts of humans and animals [2][3][4]. K. pneumoniae can be detected in 62.1% of stool specimens from healthy adults [5].…”

Section: Introductionmentioning

confidence: 99%

The role of EII complex in the bacterial responses to the glucose-survey in clinical Klebsiella pneumoniae isolates

Horng,

Panjaitan,

Tsai

et al. 2023

PLoS ONE

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Type 3 fimbriae in Klebsiella pneumoniae are important for bacterial colonization on abiotic and biotic surfaces. The major subunit of type 3 fimbriae (MrkA) is increased by overexpression of EtcABC, an EII complex of phosphoenolpyruvate:carbohydrate phosphotransferase systems (PTSs), through cAMP-cAMP receptor protein (cAMP-CRP) in K. pneumoniae STU1. Here, we further characterized the relations between the amount of etcABC mRNA and MrkA in 78 clinical K. pneumoniae isolates incubated in high levels of glucose. By Western blotting, we observed that MrkA of 29 isolates were not decreased much by high levels of glucose (Group A) but MrkA of other 49 isolates were significantly reduced (Group B) in the same condition. The bacterial biofilms on abiotic surfaces and colonization in the Caenorhabditis elegans of representative isolates in the Group A were not affected by high levels of glucose. However, the biofilm and colonization in the worm of clinical isolates in the Group B were much reduced by high levels of glucose. After quantification by real time RT-PCR, 76% of Group A but just 10% of Group B showed high amount of etcA mRNA. In summary, our results suggested that for most of K. pneumoniae clinical isolates, the amount of etcABC mRNA was positively related to their type 3 fimbriae production in a high level of glucose, thereby to their biofilm formation and colonization in the worm.

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Prevalence of antimicrobial resistance and virulence genes in Klebsiella pneumoniae and Congenetic Raoultella Isolates from captive giant pandas

Cited by 3 publications

References 55 publications

The Impact of Bamboo Consumption on the Spread of Antibiotic Resistance Genes in Giant Pandas

The Impact of Bamboo Consumption on the Spread of Antibiotic Resistance Genes in Giant Pandas

Clostridium butyricum isolated from giant panda can attenuate dextran sodium sulfate-induced colitis in mice

The role of EII complex in the bacterial responses to the glucose-survey in clinical Klebsiella pneumoniae isolates

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