Prevalence of antimicrobial resistance and integron gene cassettes in Escherichia coli isolated from yaks (Poephagus grunniens) in Aba Tibetan Autonomous Prefecture, China

Yang, Xin; Zou, Wencheng; Zeng, Jinxin; Xie, Shengze; An, Tianwu; Luo, Xiaolin; Chen, Danyu; Feng, Lan; Cheng, Guangyang; Cai, Run; Huang, Qianru; Wang, Hongning

doi:10.1016/j.micpath.2017.09.008

Cited by 24 publications

(9 citation statements)

References 31 publications

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“…This arrangement has been more frequently described in Gram-negative bacteria of the genera Salmonella spp., and Escherichia spp. [14,15]. It was originally identified in several isolates in the early 90's.…”

Section: Discussionmentioning

confidence: 99%

“…Integrons can be classified into three classes [1][2][3], based on the sequence of the integrase gene, of which it is known that class 1 integrons are the most frequently detected in strains of clinical origin, and are associated with resistance to multiple antibiotics. More than 50 different class 1 integrons and 60 gene cassettes have been described, including gene cassettes that confer resistance to aminoglycosides, penicillins, cephalosporins, carbapenems, trimethoprim, chloramphenicol, rifampicin, erythromycin, and quaternary ammonium compounds [13][14][15][16][17]. Under this context, the objective of this work is to provide experimental evidence through the identification of the structural diversity of class 1 integrons, phylogenetic classification and serotyping, which allows to locate E. coli as possible primary pathogen causing AV, for the possible implementation of routine studies for the detection of E. coli causing vaginal infection.…”

Section: Introductionmentioning

confidence: 99%

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Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons

Blancarte-Lagunas

Castro-Escarpulli

Navarro

et al. 2020

J Infect Dev Ctries

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Introduction: Antimicrobial resistance in Escherichia coli, one of the causal agents of aerobic vaginitis, leads to the persistence of the infection. The investigation of integrons acquires relevance, since they are elements that are responsible for the acquisition of resistance to antibiotics. The aim of this work was to describe the structural diversity of class 1 integrons in virulent and commensal strains of E. coli isolated from patients with vaginal infection. Methodology: Ninety-two strains of E. coli were isolated from patients with aerobic vaginitis. Resistance profile against 19 antibiotics and class 1 integrons were detected by PCR. The identity and arrangement of cassettes was determined by sequencing. ERIC-PCR assays were carried out in strains with identical arrays. Finally, genotyping by Clermont algorithm and serotyping were performed. Seventeen strains showed integrons located in plasmids. Results: Ten different gene cassette arrays were identified in 30 strains of E. coli. Cassettes corresponding to genes coding for adenylyltransferases (aadA), dihydrofolate reductases (dfrA), and oxacillinases (blaOXA) were detected. Array dfrA17-aadA5 was predominantly prevalent over the other arrays identified. Phylogenetic group A was the most predominant, followed by group B2 and D. Conclusions: This study demonstrates the presence of E. coli of vaginal origin carrying class 1 integrons, which are main genetic elements of capture of resistance genes, with the possibility of capturing new resistance cassettes. These evidences should serve for the modification of protocols in the diagnosis and treatment of aerobic vaginitis, and the development of policies for the rational use of antimicrobials.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons

Blancarte-Lagunas

Castro-Escarpulli

Navarro

et al. 2020

J Infect Dev Ctries

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“…Unfortunately, few studies of antimicrobials on poultry origin E. coli isolated from this region were reported in the past. However, 46.7~80.0% of E.coli isolates identified from the fresh faeces of yaks was found to show highly multi-drug resistance capabilities (MDR) in Tibet [20,21]. Furthermore, the Tibet strains in this study was much more resistant to tetracycline (100%), doxycycline (100%), sulfisoxazole (100%), ampicillin (50%) and amoxicillin/clavulanic acid (50%), but high sensitivity to gentamicin (0%), spectinomycin (0%), trimethoprim-sulfamethoxazole (0%), cefotaxime (0%), ceftazidime (0%), florfenicol (0%), enrofloxacin (0%), ofloxacin (0%), meropenem (0%) and colimycin (0%).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the Tibet strains in this study was much more resistant to tetracycline (100%), doxycycline (100%), sulfisoxazole (100%), ampicillin (50%) and amoxicillin/clavulanic acid (50%), but high sensitivity to gentamicin (0%), spectinomycin (0%), trimethoprim-sulfamethoxazole (0%), cefotaxime (0%), ceftazidime (0%), florfenicol (0%), enrofloxacin (0%), ofloxacin (0%), meropenem (0%) and colimycin (0%). Nevertheless, the isolates of Yak origin showed different level of resistance to ampicillin (90.9%), doxycycline (90.9%), tetracycline (81.8%), cefotaxime (79%), gentamicin (63.4%) and trimethoprimsulfamethoxazole (54%) [20,21]. These difference of antimicrobials maybe connect with a large amount of yaks pasture and few poultry farming in Tibet autonomous region.…”

Section: Discussionmentioning

confidence: 99%

Virulence and Antimicrobial Characteristics of Escherichia Coli Isolated from Diseased Chickens in China and Algeria

Wang¹,

Zhao

Gao

et al. 2019

JAA

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To reveal and compare the prevalence of pathotypes and virulence genes, as well as antimicrobial resistance and genotyping of poultry E. coli isolates from China and Algeria. Pathotype and seven virulence genes were tested by PCR, susceptibility to antimicrobials was evaluated using broth microdilution method, and genotyping was analyzed by PFGE. Six isolates were identified as pathogenic E. coli. Virulence gene testing showed that the frequency of ompT, iss, fimC, iroN, hlyF and iutA was high in the isolates from Shandong, Shanxi, Jiangsu and Xinjiang province, while stx2 was detected in only two isolates from Shandong, and stx2, iss, fimC, hlyF and iutA could not be detected in Tibet isolates. Importantly, nearly all isolates from Algeria carried seven virulence genes. Drug resistance testing of 141 strains showed that 98.2% (109/111) of the isolates from China and all isolates (30/30) from Algeria resist to more than three classes of antimicrobials. The PFGE genotyping of 157 isolates yielded 134 types, demonstrated a high level of genetic diversity among these isolates. Thus, the poultry E. coli from both China and Algeria exhibited either high frequencies of antimicrobial resistance or high rates of virulence genes carrying.

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“…Integrons are strongly associated with the dissemination of antimicrobial genes among different genera of bacteria [2]. Several types of integron classes have been found nowadays in bacteria [3]. Integrons are often located on transmissible plasmids or transposons, which can facilitate the transferability of antimicrobial genes among bacteria [4].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Huang

Lan

et al. 2020

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Background. Escherichia coli sequence type 131 (ST131) is an important multidrug-resistant extraintestinal pathogen, which can cause many kinds of infections. Integrons may play a crucial role in the dissemination of antibiotic resistance genes. e purpose of this study was to characterize the prevelance of integrons among E. coli ST131 strains in China. Methods. Eighty-three E. coli ST131 isolates were used in this study. e antibiotic susceptibility test was performed by the disk diffusion method. e presence and characterization of class 1, 2, and 3 integrons, as well as promotor of gene cassettes and other antimicrobial resistance genes, were detected by PCR and DNA sequencing. Transfer of integrons was carried out using a broth culture mating method. Clonal relatedness of E. coli ST131 isolates was analyzed by PFGE. Results. Overall, 26.5% (22/83) of the E. coli ST131 isolates carried class 1 integrons. Class 2 and 3 integrons were not found in this study. Two types of gene cassette arrays were demonstrated in this study and were as follows: dfrA17-aadA5 and aac(6′)-Ib-cr-cmlA5. Only one type of Pc promoter variant was detected among 22 integron-positive isolates (PcW). In vivo transfer of integron was successful for 9 of integron-positive E. coli ST131 isolates harboring resistance gene cassettes. Results of PFGE demonstrated that the integron-positive E. coli ST131 isolates were grouped into 12 different PFGE clusters. Conclusions. Our study showed a low prevalence of integrons was detected in E. coli ST131. Continued surveillance of this mobile genetic element should be performed to study the evolution of antibiotic resistance among E. coli ST131.

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Prevalence of antimicrobial resistance and integron gene cassettes in Escherichia coli isolated from yaks (Poephagus grunniens) in Aba Tibetan Autonomous Prefecture, China

Cited by 24 publications

References 31 publications

Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons

Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons

Virulence and Antimicrobial Characteristics of Escherichia Coli Isolated from Diseased Chickens in China and Algeria

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

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