Prevalence of antibodies against Kaposi's sarcoma associated herpes virus (KSHV) complement inhibitory protein (KCP) in KSHV-related diseases and their correlation with clinical parameters

Okrój, Marcin; Tedeschi, Rosamaria; Mancuso, Roberta; Brambilla, Lucia; Tourlaki, Athanasia; Dillner, Joakim; Blom, Anna M.

doi:10.1016/j.vaccine.2010.11.070

Cited by 5 publications

(7 citation statements)

References 14 publications

(12 reference statements)

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“…ORF4, a viral glycoprotein with heparan sulfate binding and complement inhibition activity ( 34 , 35 ), is one major target of VLV immune serum. Serum containing anti-ORF4 antibodies has been demonstrated to antagonize its complement inhibition function ( 36 ). Thus, we hypothesized that the anti-ORF4 antibodies elicited by VLVs could make KSHV susceptible to neutralization by the complement system.…”

Section: Resultsmentioning

confidence: 99%

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Immunization of Mice with Virus-Like Vesicles of Kaposi Sarcoma-Associated Herpesvirus Reveals a Role for Antibodies Targeting ORF4 in Activating Complement-Mediated Neutralization

Lam

Roshan

Miley

et al. 2023

J Virol

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Section: Resultsmentioning

confidence: 99%

“…The contribution of ORF4-specific antibodies in the sera of KSHV-infected individuals to complement-mediated neutralization has not yet been studied ( 36 , 41 ). K8.1-specific antibodies are the predominant antibodies in infected individuals, while ORF4-specific antibodies are much rarer and lower in quantity ( 22 , 41 ).…”

Section: Resultsmentioning

confidence: 99%

Immunization of Mice with Virus-Like Vesicles of Kaposi Sarcoma-Associated Herpesvirus Reveals a Role for Antibodies Targeting ORF4 in Activating Complement-Mediated Neutralization

Lam

Roshan

Miley

et al. 2023

J Virol

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“…KSHV encodes viral IL-6, which directly binds to gp130, and activates MAPK serine/threonine kinase pathways (Cousins and Nicholas 2013; Molden et al 1997). To interfere with the complement system, ORF4, also called complement control protein (KCP), blocks progression of the complement cascade (Okroj et al 2011; Spiller et al 2003). KSHV encodes four viral homologs of IRF (vIRF1–4) (Baresova et al 2013).…”

Section: Kshv Life Cycle and Oncogenesismentioning

confidence: 99%

Towards Better Understanding of KSHV Life Cycle: from Transcription and Posttranscriptional Regulations to Pathogenesis

et al. 2019

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Kaposi’s sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus-8 (HHV-8), is etiologically linked to the development of Kaposi’s sarcoma, primary effusion lymphoma, and multicentric Castleman’s disease. These malignancies often occur in immunosuppressed individuals, making KSHV infection-associated diseases an increasing global health concern with persistence of the AIDS epidemic. KSHV exhibits biphasic life cycles between latent and lytic infection and extensive transcriptional and posttranscriptional regulation of gene expression. As a member of the herpesvirus family, KSHV has evolved many strategies to evade the host immune response, which help the virus establish a successful lifelong infection. In this review, we summarize the current research status on the biology of latent and lytic viral infection, the regulation of viral life cycles and the related pathogenesis.

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“…Furthermore, studies have shown that the humoral response to viruses such as KSHV is complex (15), and appropriate serological assays may require approaches that detect multiple antigens simultaneously. Other proteins, such as the complement inhibitory protein (KCP) and viral cyclin, have been shown to be useful targets for serological screening of KSHV infections (14,41). The Luminex assays that we have developed use a complex mixture of more than 19 viral antigens present in purified virions, including the KSHV ORF65 capsid protein and the K8.1 virion glycoprotein in the RV1 assay and the MneRV2 homologs of these proteins in the RV2 assay.…”

Section: Figmentioning

confidence: 99%

Development of Whole-Virus Multiplex Luminex-Based Serological Assays for Diagnosis of Infections with Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 Homologs in Macaques

Ryan¹,

Rose

2013

Clin. Vaccine Immunol.

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bKaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus 8 is a tumorigenic rhadinovirus that is associated with all forms of Kaposi's sarcoma. Current serological detection of KSHV is based on enzyme-linked immunosorbent or immunofluorescence assays that suffer from a variety of problems, including the lack of defined standards for test comparison. While KSHV is the only known human rhadinovirus, two lineages of KSHV-like rhadinoviruses are found in Old World primates: the RV1 lineage includes KSHV and retroperitoneal fibromatosis herpesvirus (RFHV) in macaques, and the RV2 lineage includes RRV and MneRV2 from different macaque species. To develop animal models of KSHV-associated diseases, we developed quantitative multiplex bead-based serological assays to detect antibodies against rhadinovirus antigens. Proteins from KSHV (RV1) and MneRV2 (RV2) virions were coupled to spectrally distinct fluorescent beads and used in Luminex flow cytometry-based assays to detect immune responses in macaques. Both assays showed large dynamic ranges with high levels of seroreactivity to both KSHV and MneRV2 proteins. A large set of macaque serum samples from the Washington National Primate Research Center was screened, and most of the samples (82%) were positive in both assays, consistent with the high level of RV1-RV2 coinfection detected by PCR. The macaque sera showed broad, variable, and unique serological responses to the different viral antigens, allowing an initial seroprevalence to be determined for the macaque viruses. The Luminex assays offer a novel multiplexed approach to assess rhadinovirus infection patterns in both humans and nonhuman primates. This will help advance our understanding of rhadinovirus biology and associated host immunological responses.

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Prevalence of antibodies against Kaposi's sarcoma associated herpes virus (KSHV) complement inhibitory protein (KCP) in KSHV-related diseases and their correlation with clinical parameters

Cited by 5 publications

References 14 publications

Immunization of Mice with Virus-Like Vesicles of Kaposi Sarcoma-Associated Herpesvirus Reveals a Role for Antibodies Targeting ORF4 in Activating Complement-Mediated Neutralization

Immunization of Mice with Virus-Like Vesicles of Kaposi Sarcoma-Associated Herpesvirus Reveals a Role for Antibodies Targeting ORF4 in Activating Complement-Mediated Neutralization

Towards Better Understanding of KSHV Life Cycle: from Transcription and Posttranscriptional Regulations to Pathogenesis

Development of Whole-Virus Multiplex Luminex-Based Serological Assays for Diagnosis of Infections with Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 Homologs in Macaques

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