Prevalence of Antibiotic-Resistant Shigella spp. in Bangladesh: A Systematic Review and Meta-Analysis of 44,519 Samples

Ahmed, Saleh; Chowdhury, Md Imrul Hasan; Sultana, Sonia; Alam, Sayeda Sadia; Marzan, Mahfuza; Islam, Asiful

doi:10.3390/antibiotics12050817

Cited by 7 publications

(3 citation statements)

References 61 publications

(52 reference statements)

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“…In a recent meta-analysis, the prevalence of mecillinam resistance was measured at 13.7% (95% Confidence Interval 5.5–34.1) among 20,485 Shigella spp. clinical isolates 33 . Our work reports the occurrence of CTX-M-127 and has proven these evidences of mecillinam resistance in Bangladesh.…”

Section: Resultsmentioning

confidence: 99%

CTX-M-127 with I176F mutations found in bacteria isolates from Bangladeshi circulating banknotes

Ali,

Srinivasan,

Akter

2024

Sci Rep

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Extended-spectrum beta-lactamase (ESBL)-producing organisms are widely recognized as clinically relevant causes of difficult-to-treat infections. CTX-M has formed a rapidly growing family distributed worldwide among a wide range of clinical bacteria, particularly members of Enterobacteriaceae. Circulating banknotes, exchanged daily among people, pose a potential vehicle for transmitting multidrug resistance. We screened for ESBL-carrying bacteria in the present study and reported CTX-M mutations in Bangladesh's banknotes. We sequenced the genes and performed homology modeling using the Swiss model with CTX-M-15 (4HBT) as a template. Then, we performed molecular docking of mecillinam with the template and the generated model using Autodock 4.2 (Release 4.2.6). After docking, we visually inspected the complexes built using Autodock tools for polar contacts and pi-pi interactions in PyMOL 2.5.4. Our partially sequenced blaCTX-M was related to blaCTX-M-10 and blaCTX-M-15. We observed multiple single-nucleotide substitution mutations, i.e., G613T (silent mutation), A626T (I176F), and A503G (N135D). Homology modeling showed high similarity when the model was superimposed over the template. The orientation of Asn (135) in the template and Asp (135) in the model does not show a significant difference. Likewise, Ile (176) in the template and Phe (176) in the model offer the same orientation. Our generated model could bind to Lys237, Ser240, and Asp135 residues with the lowest binding energy on docking. Our predicted binding of the mecillinam to the mutated D-135 residue in the model indicates contributions and supports previous reports proposing CTX-M-15 to CTX-M-127 mutational conversion on the mecillinum resistance phenotype.

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Section: Resultsmentioning

confidence: 99%

CTX-M-127 with I176F mutations found in bacteria isolates from Bangladeshi circulating banknotes

Ali,

Srinivasan,

Akter

2024

Sci Rep

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“…The ultimate score of each article was determined based on the percentage of 'Yes' responses. Studies were classified as high risk of bias (low quality), moderate risk of bias (moderate quality), or low risk of bias (high quality) based on the overall scores of ≤49%, 50-69%, and ≥70%, respectively [23,24].…”

Section: Quality Assessmentmentioning

confidence: 99%

Vitamin D Status in Patients with Primary Antiphospholipid Syndrome (PAPS): A Systematic Review and Meta-Analysis

Islam,

Ahmed,

Sultana

et al. 2024

Antibodies

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Primary antiphospholipid syndrome (PAPS) is a systemic autoimmune disorder, characterised by consistently high levels of antiphospholipid antibodies, thrombosis, and/or pregnancy morbidity. Due to various suspected causes, deficient or insufficient levels of vitamin D in the serum have been reported in patients with PAPS; however, the reports have been sporadic and inconclusive. This systematic review and meta-analysis aimed to comprehensively evaluate the serum vitamin D levels in patients with PAPS compared to controls. A protocol was registered in PROSPERO (Registration No. CRD42019132128) and a systematic literature search was conducted through Google Scholar, PubMed, Web of Science, Scopus, and ScienceDirect databases without restricting language and year. Pooled prevalence, mean difference (MD), and odds ratio (OR) along with 95% confidence intervals (CI) were determined by using a random effects model. Study quality was assessed by the Joana Brigg’s Institute (JBI) protocol and publication bias was evaluated by a trim and fill funnel plot, Begg’s, and Egger’s tests. The pooled prevalence of vitamin D deficiency and insufficiency was found to be 32.2% [95% CI: 16.3–48.2] and 61.5% [95% CI: 40.2–82.8], respectively. Serum levels of vitamin D were considerably lower in the PAPS patients compared to controls (MD: −5.75, 95% CI: −9.73 to −1.77; p = 0.005). Multiple sensitivity analyses showed that the results remained statistically significant, demonstrating the robustness of this meta-analysis. No significant publication bias was detected in determining the MD of serum vitamin D levels in PAPS and controls. In conclusion, PAPS patients had greater rates of vitamin D deficiency or insufficiency, higher frequency of thrombosis, and lower serum vitamin D levels than healthy individuals.

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“…Another study conducted in Bangladesh has shown that Shigella was significantly higher in rural areas than in urban sites [ 34 ]. A metanalysis of the updated and comprehensive assessment of the shigellosis drug resistance burden in Bangladesh demonstrated a higher prevalence rate for multidrug-resistant Shigella species, and the resistance rates were 61.9% for fluoroquinolone, 60.8% for trimethoprim-sulfamethoxazole, 38.8% for azithromycin, 36.2% for nalidixic acid, 34.5% for ampicillin, and 31.1% for ciprofloxacin [ 35 ].…”

Section: Historical Shigella Incidence Prevalence ...mentioning

confidence: 99%

The Enterics for Global Health (EFGH) Shigella Surveillance Study in Bangladesh

Khanam,

Islam,

Bhuiyan

et al. 2024

Open Forum Infectious Diseases

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Background Shigella is an important cause of diarrhea in Bangladeshi children <5 years of age, with an incidence rate of 4.6 per 100 person-years. However, the report was more than a decade old, and data on Shigella consequences are similarly outdated and heterogeneously collected. Methods Facility-based disease surveillance is planned to be carried out under the Enterics for Global Health (EFGH) Shigella Surveillance Study consortium for 2 years with aims to optimize and standardize laboratory techniques and healthcare utilization and coverage survey, clinical and anthropometric data collection, safety monitoring and responsiveness, and other related activities. The EFGH is a cohesive network of multidisciplinary experts, capable of operating in concert to conduct the study to generate data that will pave the way for potential Shigella vaccine trials in settings with high disease burden. The study will be conducted within 7 country sites in Asia, Africa, and Latin America. Conclusions We outline the features of the Bangladesh site as part of this multisite surveillance network to determine an updated incidence rate and document the consequences of Shigella diarrhea in children aged 6–35 months, which will help inform policymakers and to implement the future vaccine trials.

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Prevalence of Antibiotic-Resistant Shigella spp. in Bangladesh: A Systematic Review and Meta-Analysis of 44,519 Samples

Cited by 7 publications

References 61 publications

CTX-M-127 with I176F mutations found in bacteria isolates from Bangladeshi circulating banknotes

CTX-M-127 with I176F mutations found in bacteria isolates from Bangladeshi circulating banknotes

Vitamin D Status in Patients with Primary Antiphospholipid Syndrome (PAPS): A Systematic Review and Meta-Analysis

The Enterics for Global Health (EFGH) Shigella Surveillance Study in Bangladesh

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