Prevalence of antibiotic resistance genes and bacterial pathogens along the soil–mangrove root continuum

Wang, Cheng; Hu, Ruiwen; Strong, Peter James; Zhuang, Wei; Huang, Wei‐Ming; Luo, Zhengxiu; Yan, Qingyun; He, Zhili; Shu, Longfei

doi:10.1016/j.jhazmat.2020.124985

Cited by 34 publications

(15 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found that endospheric resistomes are transmitted chiefly from the episphere and partially from the root (Figure d). This reinforces the notion that the resistome in lettuce leaves might transmit from both the exterior (through the episphere) and interior (through the root) of lettuce tissue. ,,,,, In our study, manure had a more significant impact on the microbiome and resistome in surface soil than rhizosphere soil. Therefore, we propose that the transmission route of manure–surface soil–episphere is more important than the transmission route of manure–rhizosphere soil–root in shaping the microbiome and resistome in endosphere.…”

Section: Discussionsupporting

confidence: 87%

“…Recent studies report overlaps of the microbiome and resistome among different compartments of the plant–soil ecosystem, − suggesting the possible transmission of microbes and ARGs from soil to plants. For example, overlaps in microbiome have been found between soil and the leaves and flowers of grapevine; and between the leaf endosphere and episphere of Espeletia plant .…”

Section: Introductionmentioning

confidence: 99%

“…For example, overlaps in microbiome have been found between soil and the leaves and flowers of grapevine; and between the leaf endosphere and episphere of Espeletia plant . In addition, overlaps in the resistome have been discovered between the root of lettuce and soil amended with poultry litter; between the rhizosphere soil and the phyllosphere of Brassica planted in struvite-amended soil; and between the rhizosphere soil and the root of mangrove . These previous studies often investigated a subset of the environmental compartments in the soil-plant ecosystem and focused on either microbiome or resistome.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transmission Routes of the Microbiome and Resistome from Manure to Soil and Lettuce

Sun

Snow

Walia

et al. 2021

Environ. Sci. Technol.

View full text Add to dashboard Cite

The land application of animal manure can introduce manure microbiome and resistome to croplands where food crops are grown. The objective of this study was to characterize the microbiome and resistome on and in the leaves of lettuce grown in manured soil and identify the main transmission routes of microbes and antibiotic resistance genes (ARGs) from soil to the episphere and endosphere of lettuce. Shotgun metagenomic results show that manure application significantly altered the composition of the microbiome and resistome of surface soil. SourceTracker analyses indicate that manure and original soil were the main source of the microbiome and resistome of the surface soil and rhizosphere soil, respectively. Manure application altered the microbiome and resistome in the episphere of lettuce (ADONIS p < 0.05), and surface soil accounted for ∼81% of the microbes and ∼62% of the ARGs in episphere. Manure application had limited impacts on the microbiome and resistome in the endosphere (ADONIS p > 0.05). Our results show that manure-borne microbes and ARGs reached the episphere primarily through surface soil and some epiphytic microbes and ARGs further entered the endosphere. Our findings can inform the development of pre-and postharvest practices to minimize the transmission of manure-borne resistome from food crops to consumers.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transmission Routes of the Microbiome and Resistome from Manure to Soil and Lettuce

Sun

Snow

Walia

et al. 2021

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…A Nanodrop spectrophotometer (ND-1000, NanoDrop Technologies, USA) was deployed to analyze the quality and concentration of extracted DNA. The fungal ITS1 gene was amplified by primer pair ITS1F (5′-CTTGGTCATTTAGAGGAAGTAA-3′) and ITS2 (5′-GCTGCGTTCTTCATCGATGC-3′) 54 . PCR amplifications were firstly conducted in a total volume of 25 μl using KOD FX Neo (TOYOBO, Japan) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Soil fungal communities affect the chemical quality of flue-cured tobacco leaves in Bijie, Southwest China

Wang

Zhang

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Soil microorganisms could affect the quality of tobacco leaves, however, little is known about the association of tobacco chemical components and soil fungal communities. In the present study, the relationship between soil fungi and tobacco quality based on chemical components in Bijie was investigated. The results showed that the total harmony scores (THS) of the analyzed tobacco leaves ranged from 46.55 ± 3.5 to 91.55 ± 2.25. Analyses of chemical components revealed that high contents of nicotine (≥ 1.06%) and sugar (total sugar: ≥ 22.96%, reducing sugar: ≥ 19.62%), as well as low potassium level (≤ 2.68%) were the main factors limiting the quality of flue-cured tobacco leaves. Pearson correlation analysis indicated that soil nitrate, available potassium/phosphorous, and organic matter significantly correlated with tobacco nicotine, potassium, and chloride levels (p < 0.05). Besides, the analysis of alpha- and beta-diversity of soil fungal communities implied that fungal structure rather than the richness affected the chemical quality of tobacco. In detail, the relative abundance of Humicola olivacea species in soils was positively correlated with the THS of tobaccos (r = 0.52, p < 0.05). Moreover, the species including Mortierella alpina, Mortierella hyalina, Tausonia pullulan, and Humicola olivacea were negatively correlated with tobacco sugar (r ≤ − 0.45, p < 0.05) while, Codinaea acaciae and Saitozyma podzolica species were negatively correlated with tobacco nicotine (r ≤ − 0.51, p < 0.05). The present study provides a preliminary basis for utilizing fungal species in soils to improve the chemical quality of tobacco in the studied area.

show abstract

“…For the spatial transmission of ARB, the bacterium in the soil could secrete cell wall degrading enzymes to break through root barrier and enter the plant. They may also enter plant through natural wounds on the root surface or following the root infection ( Chi et al, 2005 ; Liu et al, 2017 ; Wang et al, 2021 ). For gene exchanges between soil and endophytic bacteria, ARGs on mobile genetic elements (MGEs) may transfer inside of plant when soil bacteria and vegetable endophytes closely contact with each other in the rhizosphere ( Barlow, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Nitrogen Regulates the Distribution of Antibiotic Resistance Genes in the Soil–Vegetable System

Wang

Sun

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

The increasing antibiotic resistance genes (ARGs) in fertilizer-amended soils can potentially enter food chains through their transfer in a soil–vegetable system, thus, posing threats to human health. As nitrogen is an essential nutrient in agricultural production, the effect of nitrogen (in the forms NH4+-N and NO3−-N) on the distribution of ARGs (blaTEM-1, sul1, cmlA, str, and tetO) and a mobile genetic element (MGE; tnpA-4) in a soil–Chinese cabbage system was investigated. Not all the tested genes could transfer from soil to vegetable. For transferable ones (blaTEM-1, sul1, and tnpA-4), nitrogen application influenced their abundances in soil and vegetable but did not impact their distribution patterns (i.e., preference to either leaf or root tissues). For ARGs in soil, effects of nitrogen on their abundances varied over time, and the positive effect of NH4+-N was more significant than that of NO3−-N. The ARG accumulation to vegetables was affected by nitrogen application, and the nitrogen form was no longer a key influencing factor. In most cases, ARGs were found to prefer being enriched in roots, and nitrogen application may slightly affect their migration from root to leaf. The calculated estimated human intake values indicated that both children and adults could intake 106–107 copies of ARGs per day from Chinese cabbage consumption, and nitrogen application affected ARG intake to varying degrees. These results provided a new understanding of ARG distribution in vegetables under the agronomic measures such as nitrogen application, which may offer knowledge for healthy vegetable cultivation in future.

show abstract

Prevalence of antibiotic resistance genes and bacterial pathogens along the soil–mangrove root continuum

Cited by 34 publications

References 75 publications

Transmission Routes of the Microbiome and Resistome from Manure to Soil and Lettuce

Transmission Routes of the Microbiome and Resistome from Manure to Soil and Lettuce

Soil fungal communities affect the chemical quality of flue-cured tobacco leaves in Bijie, Southwest China

Nitrogen Regulates the Distribution of Antibiotic Resistance Genes in the Soil–Vegetable System

Contact Info

Product

Resources

About