Prevalence of &amp;lt;i&amp;gt;Escherichia coli&amp;lt;/i&amp;gt; in Fish and Shrimps obtained from Retail Fish Markets in &amp; around Kolkata, India

Dutta, Chandraval

doi:10.11648/j.fem.20160201.11

Cited by 7 publications

(7 citation statements)

References 7 publications

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“…Biofilm formation on food contact surfaces and the prevalence of virulent and MDR E. coli in it poses food safety issues in the seafood pre-processing plants. Even though the prevalence of E. coli in seafood from India has been previously reported (Dutta and Sengupta, 2016;Gupta et al, 2013), to the best of our knowledge, the present study is the first report of the isolation and risk assessment of the E. coli from biofilms associated with food contact surfaces in seafood pre-processing centers. E. coli strains entering the food processing environment either through contaminated food or through carriers handling seafood may survive on the premises by forming a biofilm on various surfaces.…”

Section: Discussionmentioning

confidence: 72%

Prevalence, Antimicrobial Resistance, and Molecular Characterization of Escherichia coli Isolated from Food Contact Surfaces in Seafood Pre-Processing Plants (India)

Francis¹,

Antony²,

Sukumaran³

et al. 2022

JFQHC

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Background: The survival of pathogens in biofilms poses a threat to food safety. The aim of this study was to determine prevalence, antimicrobial resistance, and molecular characterization of Escherichia coli strains. Methods: Swab samples (n=144) were collected from biofilm formed on food contact surfaces in seafood pre-processing plant in India. E. coli was isolated and identified using uid A gene by Polymerase Chain Reaction (PCR). The risk assessment of the isolates was carried out in terms of their drug resistance and the presence of virulence genes. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method. Phylogenetic grouping was done by quadruplex PCR. Molecular typing of the strains was performed by Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR). Data were statistically analyzed using SPSS version 22. Results: Enteropathogenic E. coli (EPEC) strains were the most prevalent serotype. Multiplex PCR analysis revealed the presence of shiga toxin genes (stx1, stx2), intimin (eae), and enterohemolysin genes (hlyA). Shiga toxin gene stx2 showed the highest prevalence (83.33%). Among various phylogroups, B1 (45.56%) and B2 (30%) were the most prevalent phylogroups. Resistance to ampicillin (85.56%), piperacillin (84.44%), and cefpodoxime (85.56%) was widespread among the E. coli strains. Conclusion: The presence of genetically heterogeneous multi drug resistant E. coli strains with virulence potential showed a high risk in the seafood industry.

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Section: Discussionmentioning

confidence: 72%

Prevalence, Antimicrobial Resistance, and Molecular Characterization of Escherichia coli Isolated from Food Contact Surfaces in Seafood Pre-Processing Plants (India)

Francis¹,

Antony²,

Sukumaran³

et al. 2022

JFQHC

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“…(Table 3.2). The occurrence of E. coli in shrimp is expected since these organisms are present in the estuarine environment where contamination of faecal matter is commonly reported [13]. In this study, 58% samples of shrimp were contaminated with E. coli which is lower than E. coli load in shrimp imported to Switzerland (64%) [5], and shrimp from retail in Kolkata, India (85%) [13], but higher than shrimp from Tuticorin Coast, South-eastern India (10%) [19].…”

Section: Discussionmentioning

confidence: 75%

“…Presence of E. coli indicates poor hygiene and sanitary condition. Quality of seafood depends on the hygiene condition of the landing areas and the quality of water where the shrimp is farmed [13]. E. coli are not part of microflora in shrimp; therefore, the presence of E. coli might indicate a sign of faecal contamination from human or animal in the aquaculture environment and also crosscontamination that might occur during handling before it reaches the retail market and includes display time before it is bought by consumers [5].…”

Section: Identification Of Bacteriamentioning

confidence: 99%

Antimicrobial Resistance of Staphylococcus aureus and Escherichia Coli Isolated from Local Shrimp

Syuhada¹,

Syifa²,

M.A.R.³

et al. 2019

IJET

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Staphylococcus aureus and Escherichia coli are pathogens that can cause foodborne illnesses due to their ability to produce toxin. These bacteria are not naturally found in seafood, but recent studies have shown the prevalence of this microorganism in shrimp and its antibiotic resistance properties. In this study, shrimp from two species; Litopenaeus vannamei and Penaeus monodon were obtained to assess the prevalence of S. aureus and E. coli and to test their antibiotics susceptibility. The result obtained by isolation indicated that 87% and 58% of shrimp samples were contaminated with S. aureus and E. coli, respectively. S. aureus and E. coli occurrence in shrimps from retail for twelve weeks fluctuated in range between 2.00 to 4.94 log10 CFU/g and 2.00 to 6.39 log10 CFU/g, respectively. The identified bacteria were subjected to antibiotic susceptibility test. The result showed that most of S. aureus and E. coli isolates were resistant to penicillin G from β-lactam antibiotic group. Multidrug-resistant organism was identified in two isolates of S. aureus and seven isolates of E. coli. This indicated the need of improving handling of shrimp by food handlers and monitoring activities for S. aureus and E. coli contamination in shrimp as a preventive measure to ensure the safety of public health.

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“…The percentages may indicate the probability of the presence of these bacteria in higher density in freshwater. High E. coli load like 80.70%, 46.6% and 80% was also found in India (Dutta, 2016), Kenya (Onyuka et al 2011), andSwitzerland (Boss et al, 2016), respectively. The prevalence of coliforms and fecal coliforms at higher ranges may indicate sewage contamination.…”

Section: Fig 4 Growth Of E Coli Colonies On Emb Agar Platementioning

confidence: 85%

“…A large group of the population involved in unhygienic sanitary practices mostly live beside the haor, baor, beel, pond, and river. Open defecation and inadequately treated or untreated domestic sewage disposal may contaminate these natural water bodies with several human pathogenic microbes (Dutta, 2016). In most cases, domestic local markets' surroundings remain soggy, filthy, and unhealthy (Begum et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and distribution of antimicrobial resistance profile of Escherichia coli isolated from various local fish markets in Dhaka city, Bangladesh

Akter

Abedin

Mosharaf

et al. 2022

J Bangladesh Acad Sci

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Considering the importance of the fish and fish products as a vital part of the human diet in Bangladesh, the study aimed to estimate the microbial load, identify the E. coli and observe the isolates antibiogram pattern. Standard plate count technique, as well as the 3-tube most probable number (MPN) method, were applied to estimate the total viable bacteria (TVB) and total coliforms (TC), total fecal coliforms (TFC), and E. coli, respectively. Approximately 61.91% of samples fell into the marginally acceptable limit for TVB count, while 66.67% and 95.25% of examined species exceeded the threshold limit for TC and TFC, respectively (p<0.05). Around 90% of samples were contaminated with E. coli. A culture sensitivity test revealed that cent percent strains harbored resistance to penicillin, rifampicin, and erythromycin. Multiple antibiotic resistance (MAR) index above 0.2 may indicate the misuse or overuse of antibiotics. The findings highlight the potential food safety hazards associated with fish concerning the random use of antibiotics. J. Bangladesh Acad. Sci. 46(1); 9-18: June 2022

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Prevalence of <i>Escherichia coli</i> in Fish and Shrimps obtained from Retail Fish Markets in & around Kolkata, India

Cited by 7 publications

References 7 publications

Prevalence, Antimicrobial Resistance, and Molecular Characterization of Escherichia coli Isolated from Food Contact Surfaces in Seafood Pre-Processing Plants (India)

Prevalence, Antimicrobial Resistance, and Molecular Characterization of Escherichia coli Isolated from Food Contact Surfaces in Seafood Pre-Processing Plants (India)

Antimicrobial Resistance of Staphylococcus aureus and Escherichia Coli Isolated from Local Shrimp

Prevalence and distribution of antimicrobial resistance profile of Escherichia coli isolated from various local fish markets in Dhaka city, Bangladesh

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