Prevalence of American foulbrood in asymptomatic apiaries of Kurdistan, Iran

Khezri, Mohammad; Moharrami, Mojtaba; Modirrousta, H.; Torkaman, M.; Rokhzad, Babak; Khanbabaie, Homan

doi:10.14202/vetworld.2018.281-285

Cited by 8 publications

(6 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, detection of P . larvae in brood and adult honeybee samples in asymptomatic colonies was not uncommon [ 25 – 27 ]. P .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Microbiota dysbiosis in honeybee ( Apis mellifera L . ) larvae infected with brood diseases and foraging bees exposed to agrochemicals

Fan

et al. 2021

R. Soc. open sci.

View full text Add to dashboard Cite

American foulbrood (AFB) disease and chalkbrood disease (CBD) are important bacterial and fungal diseases, respectively, that affect honeybee broods. Exposure to agrochemicals is an abiotic stressor that potentially weakens honeybee colonies. Gut microflora alterations in adult honeybees associated with these biotic and abiotic factors have been investigated. However, microbial compositions in AFB- and CBD-infected larvae and the profile of whole-body microbiota in foraging bees exposed to agrochemicals have not been fully studied. In this study, bacterial and fungal communities in healthy and diseased (AFB/CBD) honeybee larvae were characterized by amplicon sequencing of bacterial 16S rRNA gene and fungal internal transcribed spacer1 region, respectively. The bacterial and fungal communities in disordered foraging bees poisoned by agrochemicals were analysed. Our results revealed that healthy larvae were significantly enriched in bacterial genera Lactobacillus and Stenotrophomonas and the fungal genera Alternaria and Aspergillus . The enrichment of these microorganisms, which had antagonistic activities against the etiologic agents for AFB and CBD, respectively, may protect larvae from potential infection. In disordered foraging bees, the relative abundance of bacterial genus Gilliamella and fungal species Cystofilobasidium macerans were significantly reduced, which may compromise hosts' capacities in nutrient absorption and immune defence against pathogens. Significantly higher frequency of environmentally derived fungi was observed in disordered foraging bees, which reflected the perturbed microbiota communities of hosts. Results from PICRUSt and FUNGuild analyses revealed significant differences in gene clusters of bacterial communities and fungal function profiles. Overall, results of this study provide references for the composition and function of microbial communities in AFB- and CBD-infected honeybee larvae and foraging bees exposed to agrochemicals.

show abstract

“…In fact, detection of P . larvae in brood and adult honeybee samples in asymptomatic colonies was not uncommon [ 25 – 27 ]. P .…”

Section: Discussionmentioning

confidence: 99%

“…In fact, detection of P. larvae in brood and adult honeybee samples in asymptomatic colonies was not uncommon [25][26][27]. P. larvae may exist as a pathobiont in the microbiota of worker bees, from where it is transmitted to broods [13].…”

Section: Discussionmentioning

confidence: 99%

Microbiota dysbiosis in honeybee ( Apis mellifera L . ) larvae infected with brood diseases and foraging bees exposed to agrochemicals

Fan

et al. 2021

R. Soc. open sci.

View full text Add to dashboard Cite

show abstract

“…Finally, two Paenibacillus genera were detected in the rare microbiome, a known honeybee pathogen (American foulbrood) 7 . This is likely the result of foulbrood samples within C -though recent studies reporting asymptomatic foulbrood 57 , nosema 23 , crithidia 58 and chronic paralysis virus 58 presence do raise the question of whether Paenibacillus might have been present in other studies. A more complete bee microbiome dataset might clarify this issue -particularly given that this would have significant impacts for both wild and captive bee management.…”

Section: B) the Core Microbiome At Order Level The Y-axis Indicates The Genus (A) And Order (B) While The X-axis Denotes The Detection Thmentioning

confidence: 96%

A Meta-Analysis of the 16S-rRNA Gut Microbiome Data in Honeybees (Apis Mellifera)

Gkantiragas

Gabrielli

2021

Preprint

View full text Add to dashboard Cite

Honeybees (Apis Mellifera) perform an essential role in the ecosystem and economy through pollination of insect-pollinated plants, but their population is declining. Many causes of honeybees' decline are likely to be influenced by the microbiome which is thought to play an important role in bees and is particularly susceptible to infection and pesticides. However, there has been no systematic review or meta-analysis on honeybee microbiome data. Therefore, we conducted the first systematic meta-analysis of 16S-rRNA data to address this gap in the literature. Four studies were in a usable format - accounting for 336 honeybee's worth of data - the largest such dataset to the best of our knowledge. We analysed these datasets in QIIME2 and visualised the results in R-studio. For the first time, we conducted a multi-study evaluation of the core and rare bee microbiome and confirmed previous compositional microbiome data. We established that Snodgrassella, Lactobacillus, Bifidobacterium, Fructobacillus and Saccaribacter form part of the core microbiome and identify 251 rare bacterial genera. Additional components of the core microbiome were likely obscured by incomplete classification. Future studies should refine and add to our existing dataset to produce a more conclusive and high-resolution portrait of the honeybee microbiome. Furthermore, we emphasise the need for an actively curated dataset and enforcement of data sharing standards.

show abstract

“…Tube cells are not able to proliferate and, with the help of flagella, penetrate the body cavity and multiply in hemolymph. Bee larvae fall victim to a systemic bacterial infection and die [ 172 ]. They break down into a brown, sticky liquid.…”

Section: Microorganisms As One Of the Factors Triggering Ccdmentioning

confidence: 99%

“…Some antibiotics only affect the vegetative form of bacteria and antibiotic residues can be detected in bee products such as honey or propolis [ 175 ]. Regular monitoring of colony health is a significant factor in American foulbrood prevention and essential oils are often used to control it [ 172 ].…”

Section: Microorganisms As One Of the Factors Triggering Ccdmentioning

confidence: 99%

Effects of Insecticides and Microbiological Contaminants on Apis mellifera Health

Leska,

Nowak,

Nowak

et al. 2021

Molecules

View full text Add to dashboard Cite

Over the past two decades, there has been an alarming decline in the number of honey bee colonies. This phenomenon is called Colony Collapse Disorder (CCD). Bee products play a significant role in human life and have a huge impact on agriculture, therefore bees are an economically important species. Honey has found its healing application in various sectors of human life, as well as other bee products such as royal jelly, propolis, and bee pollen. There are many putative factors of CCD, such as air pollution, GMO, viruses, or predators (such as wasps and hornets). It is, however, believed that pesticides and microorganisms play a huge role in the mass extinction of bee colonies. Insecticides are chemicals that are dangerous to both humans and the environment. They can cause enormous damage to bees’ nervous system and permanently weaken their immune system, making them vulnerable to other factors. Some of the insecticides that negatively affect bees are, for example, neonicotinoids, coumaphos, and chlorpyrifos. Microorganisms can cause various diseases in bees, weakening the health of the colony and often resulting in its extinction. Infection with microorganisms may result in the need to dispose of the entire hive to prevent the spread of pathogens to other hives. Many aspects of the impact of pesticides and microorganisms on bees are still unclear. The need to deepen knowledge in this matter is crucial, bearing in mind how important these animals are for human life.

show abstract

Prevalence of American foulbrood in asymptomatic apiaries of Kurdistan, Iran

Cited by 8 publications

References 23 publications

Microbiota dysbiosis in honeybee ( Apis mellifera L . ) larvae infected with brood diseases and foraging bees exposed to agrochemicals

Microbiota dysbiosis in honeybee ( Apis mellifera L . ) larvae infected with brood diseases and foraging bees exposed to agrochemicals

A Meta-Analysis of the 16S-rRNA Gut Microbiome Data in Honeybees (Apis Mellifera)

Effects of Insecticides and Microbiological Contaminants on Apis mellifera Health

Contact Info

Product

Resources

About