Nanoporous structures exhibit great application potential in the biosensing, energy storage and other nanoelectronics. As a newly discovered 2D material with extraordinary properties, graphene presents the ability to hold a nanoporous structure which could overcome the inherent shortages for most of the existing nanoporous materials. While how to efficiently synthesize the nanoporous structures in graphene and how to further expand the applications of nanoporous graphene (NPG) are still big challenges. In this paper, we reviewed the recent advancements related to the synthesis and potential applications of NPGs. By analyzing the different approaches to fabricate the NPGs, and the research trends for the application realization of NPG, we aim at stimulating further research on this subject. N anopore originally refers to a vital biological feature which is generally existed in cellular membranes for recognition and transport of ions and molecules between compartments within the cell and between the cell interior and outside environment [1]. After being successfully created in biological and solid-state materials, nanoporous structures have the potentials to be used in biosensing [2][3][4], diagnostics [5,6], separation [7][8][9][10] and so on. In general, the nanopore is broadly divided into two categories: biological nanopore and solid-state nanopore. Though it was proved that most of the reported biological and solid-state nanoporous structures can offer the alternative approaches for many applications, they are still limited by some inherent shortages. For example, the biological nanopores have short life time, intrinsic instability and strict requirement, which are not favored for longterm operations. The solid-state nanopores usually have robust chemical and thermal characteristics, while it is hard for them to discriminate molecules with similar sizes but different biological characteristics [11]. What's more, the thickness of these nanopores is usually far larger than the length of nucleotide, which makes them hard to read single nucleotide information from a long chain of DNA. The sensitivity of the nanopore technology used in biosensing also needs to be further improved. Graphene, as a newly discovered material made up of single layer carbon atoms, holds a subnanometer thickness as 0.34 nm, which is comparable to the spatial interval between neighboring DNA nucleotide. So a nanoporous structure created in graphene could offer the possibility of gene sequencing at a single-base resolution. Besides, graphene was demonstrated to exhibit amazing mechanical [12][13][14], electrical properties [15][16][17], and the nanoporous graphene (NPG) could inherit most of the unique properties and even further improve them, which could promise NPG to be outstanding among the nanoporous materials for many applications. For example, the nanomesh structure created in graphene sheet could open its zero bandgap, which can be used to synthesize field effect transistors (FETs). The NPG-based FETs are able to provide saturated ...