Prevalence of acariosis in honeybee colonies of <i>Apis mellifera intermissa</i> in Algeria

Adjlane, Noureddine; Habbi-Cherifi, A.; Haddad, Nizar; Ahcen, Baz

doi:10.5958/0974-4576.2018.00075.0

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The negative results for A. woodi are unexpected, according to the supposed world-wide distribution of this parasite reported by a few studies [ 35 , 50 , 53 , 54 ]. However, other studies indicated that the prevalence and diffusion of this honey bee tracheal mite is limited and potentially related to local conditions and factors [ 55 , 56 , 57 ] and this picture might better match the results we obtained. Other studies are needed to confirm the negative results derived by our approach based of the amplification of honey DNA by two different PCR assays that have been specifically designed to detect this species [ 49 , 50 ].…”

Section: Discussionsupporting

confidence: 84%

Honey as a Source of Environmental DNA for the Detection and Monitoring of Honey Bee Pathogens and Parasites

Ribani

Utzeri

Taurisano

2020

Veterinary Sciences

View full text Add to dashboard Cite

Environmental DNA (eDNA) has been proposed as a powerful tool to detect and monitor cryptic, elusive, or invasive organisms. We recently demonstrated that honey constitutes an easily accessible source of eDNA. In this study, we extracted DNA from 102 honey samples (74 from Italy and 28 from 17 other countries of all continents) and tested the presence of DNA of nine honey bee pathogens and parasites (Paenibacillus larvae, Melissococcus plutonius, Nosema apis, Nosema ceranae, Ascosphaera apis,Lotmaria passim, Acarapis woodi, Varroa destructor, and Tropilaelaps spp.) using qualitative PCR assays. All honey samples contained DNA from V. destructor, confirming the widespread diffusion of this mite. None of the samples gave positive amplifications for N. apis, A. woodi, and Tropilaelaps spp. M. plutonius was detected in 87% of the samples, whereas the other pathogens were detected in 43% to 57% of all samples. The frequency of Italian samples positive for P. larvae was significantly lower (49%) than in all other countries (79%). The co-occurrence of positive samples for L. passim and A. apis with N. ceranae was significant. This study demonstrated that honey eDNA can be useful to establish monitoring tools to evaluate the sanitary status of honey bee populations.

show abstract