Prevalence of 7 virulence genes of &lt;i&gt;Legionella&lt;/i&gt; strains isolated from environmental water sources of public facilities and sequence types diversity of &lt;i&gt;L. pneumopila&lt;/i&gt; strains in Macau

Xiong, Lina; Zhao, Hongbo; Mo, Zunli; Shi, Lei

doi:10.5582/bst.2015.01075

Cited by 7 publications

(3 citation statements)

References 20 publications

(26 reference statements)

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“…ST1119 was found in both cooling tower water and clinical samples in Catalonia, Spain ( 75 ). ST1119 was previously found in Macau ( 76 ) and was found in lake water in Huizhou city. ST739, previously found in clinical and water samples of Japan, and both natural and man-made water samples of China ( 19 , 77 ), was found in a pond and a small stream in Shenzhen city, and a garden soil sample from Guangzhou city.…”

Section: Resultsmentioning

confidence: 67%

Presence of Viable, Clinically Relevant Legionella Bacteria in Environmental Water and Soil Sources of China

et al. 2022

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Section: Resultsmentioning

confidence: 67%

Presence of Viable, Clinically Relevant Legionella Bacteria in Environmental Water and Soil Sources of China

et al. 2022

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“…and more than 70 serotypes (ST) [ 3 ]. Among Legionella spp., Lp ST1 bacteria are responsible for the majority (80%) of all reported cases of legionellosis [ 4 ]. Lp most commonly causes Legionnaires’ disease, which can result in lung damage or death [ 5 – 7 ].…”

Section: Highlight Key Pointsmentioning

confidence: 99%

Assessment of macrolide susceptibility of Legionella pneumophila isolated from public buildings’ water systems in Turkiye

Aktas

2024

North Clin Istanbul

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OBJECTIVE Legionella pneumophila (Lp) is aerobic, non-spore forming Gram-negative bacteria, which is ubiquitous in freshwater habitats, such as rivers and hot springs, as well as colonizing artificial aquatic environments. The ability of Lp to grow intracellularly within pulmonary macrophages is a prerequisite for the development of infection. Therefore, macrolides can achieve appropriate therapeutic concentrations in eukaryotic cells, such as azithromycin. This study aimed to investigate the macrolides susceptibility of Lp. METHODS Pre-flash water samples (n=143) were collected from the public buildings (hospitals and hotels) water system in Istanbul. Colonies were confirmed as Lp ST1, ST2-14, and non-pneumophila Lp using latex agglutination kit. RESULTS 30 Lp were detected in hospital (n=23) and hotel (n=7) water systems using latex agglutination. Regardless of serotype and excluding strains without zone formation (≥256 mg/L), the main MIC values of azithromycin, erythromycin and clarithromycin were 0.61 mg/L (range 0.047–1 mg/L), 0.47 mg/L (range 0.047–1 mg/L) and 0.44 mg/L (range 0.047–1 mg/L), respectively. The MIC50 and MIC90 values for macrolides were 0.5 and 3 mg/L for azithromycin, respectively; 0.38 and 1 mg/L for erythromycin, respectively; and 0.5 and 1 mg/L for clarithromycin, respectively. We compared the MIC values of the strains for all antimicrobial agents tested without serotype discrimination. We did not find a significant difference between the MIC values of the antibiotics (p=0.16). CONCLUSION Although the data obtained from our study do not fully reflect the breakpoints, further epidemiological studies are needed to standardize MIC values.

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“…Legionella species, the major causative agents of Legionnaires' disease and Pontiac fever, are commonly found in environmental samples (Xiong et al, 2016). They have been shown to induce lung infection and cause dysfunction in other organs, such as the heart and kidney, and in the central nervous system (Xiong et al, 2015). Legionella pneumophila speci cally has been identi ed as the primary cause of Legionnaires' disease.…”

Section: Introductionmentioning

confidence: 99%

DNA aptamers specific for Legionella pneumophila: systematic evolution of ligands by exponential enrichment in whole bacterial cells

et al. 2022

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Legionella pneumophila is the major causative agent of Legionnaires' disease and Pontiac fever, which pose major public health problems. Rapid detection of L. pneumophila is important for global control of these diseases. Aptamers, short oligonucleotides that bind to targets with high a nity and speci city, have great potential for use in pathogenic bacterium detection, diagnostics, and therapy. Here, we used a whole-cell SELEX (systematic evolution of ligands by exponential enrichment) method to isolate and characterize single-stranded DNA (ssDNA) aptamers against L. pneumophila. A total of 60 ssDNA sequences were identi ed after 17 rounds of selection. Other bacterial species (Escherichia coli, Bacillus subtilis, Pseudomonas syringae, Staphylococcus aureus, Legionella quateirensis, and Legionella adelaidensis) were used for counterselection to enhance the speci city of ssDNA aptamers against L. pneumophila. Four ssDNA aptamers showed strong a nity and high selectivity for L. pneumophila, with K d values in the nanomolar range. Bioinformatic analysis of the most speci c aptamers revealed predicted conserved secondary structures that might bind to L. pneumophila cell walls. In addition, the binding of these four uorescently labeled aptamers to the surface of L. pneumophila was observed directly by uorescence microscopy. This is the rst study to use SELEX to target L. pneumophila whole cells. The aptamers identi ed in this study could be used in the future to develop medical diagnostic tools and public environmental detection assays for L. pneumophila.

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Prevalence of 7 virulence genes of <i>Legionella</i> strains isolated from environmental water sources of public facilities and sequence types diversity of <i>L. pneumopila</i> strains in Macau

Cited by 7 publications

References 20 publications

Presence of Viable, Clinically Relevant Legionella Bacteria in Environmental Water and Soil Sources of China

Presence of Viable, Clinically Relevant Legionella Bacteria in Environmental Water and Soil Sources of China

Assessment of macrolide susceptibility of Legionella pneumophila isolated from public buildings’ water systems in Turkiye

DNA aptamers specific for Legionella pneumophila: systematic evolution of ligands by exponential enrichment in whole bacterial cells

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