Prevalence of +405G>C,−1154G>A Vascular Endothelial Growth Factor Polymorphism in Breast Cancer

Abstract

Vascular endothelial growth factor (VEGF) plays an important role in the development of Breast Cancer. The aim of this study was to investigate the association of polymorphisms in the VEGF gene on prognosis of Breast Cancer patients. This study comprised 200 patients with histologically confirmed cases of Breast cancer and 200 controls. Genotyping of the VEGF gene polymorphisms at +405G>C,-1154G>A, were performed by PCR-RFLP analysis. Preoperative plasma VEGF levels were determined by ELISA. Amongst both cases and controls, the genotypic distribution of the individual SNPs were all in Hardy-Weinberg equilibrium. Mean VEGF level was significantly elevated in cases compared to controls (t = 8.248; P < 0.001). No significant association was found between +405G>C,-1154G>A VEGF polymorphism and Breast Cancer. Logistic regression analysis revealed that 405GG & 1154GG were associated with higher levels of VEGF.

Vascular endothelial growth factor +405G/C and -2578C/A polymorphisms and breast cancer risk: a meta-analysis

“…For the -2578C/A polymorphism, 7 studies from 5 articles were included, including a total of 4572 patients and 4652 controls. There were 5 studies in 3 articles with cohorts of Caucasian descent (Jin et al, 2005;Jacobs et al, 2006;Pharoah et al, 2007), 1 study of Asian descent (Sa-Nguanraksa et al, 2013), and 1 study of African descent (James et al, 2014). The distribution of genotypes in the controls was consistent with HWE in all studies.…”

“…The linkage disequilibrium of the +405G/C and -2578C/A loci of the VEGF gene might synergistically increase the risk of breast cancer (James et al, 2014). However, as there was not sufficient individual information on the genotypes of both the +405G/C and -2578C/A polymorphisms, we could not perform the combined analysis of linkage disequilibrium.…”

“…Through screening the titles and reading the abstracts and the entire articles, 9 eligible articles were selected for this meta-analysis (Jin et al, 2005;Jacobs et al, 2006;Kataoka et al, 2006;Balasubramanian et al, 2007;Pharoah et al, 2007;Oliveira et al, 2011;Sa-Nguanraksa et al, 2013;James et al, 2014;Rahoui et al, 2014). The flow chart for study selection is summarized in Figure 1.…”

“…For the +405G/C polymorphism, 9 studies were available, including a total of 6054 patients and 6170 controls. There were 4 studies with cohorts of Caucasian descent (Jin et al, 2005;Jacobs et al, 2006;Balasubramanian et al, 2007;Pharoah et al, 2007), 3 studies of Asian descent (Kataoka et al, 2006;Sa-Nguanraksa et al, 2013;James et al, 2014), 1 study of African descent (Rahoui et al, 2014), and 1 study of mixed descent (Oliveira et al, 2011). The HWE test was performed on the genotype distributions of the controls, and all studies were in agreement with HWE except Oliveira et al (2011) and Sa-Nguanraksa et al (2013).…”

Effect of polymorphisms of vascular endothelial growth factor on prognosis in osteosarcoma patients

“…VEGF is a potent regulator of angiogenesis and thus plays a role in the development and prognosis of solid tumors (Kapahi et al, 2014;James et al, 2014). Several common single-nucleotide polymorphisms (SNPs) in the VEGF gene have been reported to have a role in gene expression.…”