Prevalence, morphology, and molecular characteristics of Sarcocystis spp. in domestic goats (Capra hircus) from Kunming, China

Hu, Junjie; Liu, Tingting; Liu, Qiong; Esch, Gerald W.; Chen, Jinqing; Huang, Shengbing; Wen, Tao

doi:10.1007/s00436-016-5163-6

Cited by 40 publications

(51 citation statements)

References 28 publications

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“…In fact, microscopic Sarcocystis spp. prevalence reached 91.6% in Brazil (Bittencourt et al., ), 79.4% in Egypt (Morsy et al., ), 97.4% in Iraq (Latif, Al‐Delemi, Mohammed, Al‐Bayati, & Al‐Amiry, ), 99.4% in Iran (Shekarforoush et al., ), 52.38% in Malaysia (Kutty et al., ) and 77.3% in China (Hu et al., ). Molecular analyses revealed slightly lower prevalence, varying from 50.7% to 90.48% in Brazil and Malaysia, respectively (Bittencourt et al., ; Kutty et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Sarcocystis capracanis was found in goats' meat samples from Brazil, Egypt and Malaysia (Bittencourt et al., ; Kutty et al., ; Morsy et al., ). In another study, both S. capracanis and S. hircicanis were identified in domestic goats from Kunming City (South‐western China) (Hu et al., ). The sequence described in our study was similar to S. capracanis , identified in Kunming City, and clearly distinguishable from S. hircicanis (94% identity).…”

Section: Discussionmentioning

confidence: 99%

“…The prevalence of Sarcocystis spp. in goats is high, ranging between 77.3% and 99.4%, by microscopy and meat smears examination, respectively (Hu et al., ; Shekarforoush, Razavi, Dehghan, & Sarihi, ). Sarcocystis capracanis was also reported in Brazil (Bahia State), Egypt (Cairo), Iraq (Sulaimany) and Malaysia (Selangor State) (Barham, Stützer, Karanis, Latif, & Neiss, ; Bittencourt et al., ; Kutty et al., ; Morsy et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…Sarcocystis capracanis was also reported in Brazil (Bahia State), Egypt (Cairo), Iraq (Sulaimany) and Malaysia (Selangor State) (Barham, Stützer, Karanis, Latif, & Neiss, ; Bittencourt et al., ; Kutty et al., ; Morsy et al., ). In China, both S. capracanis and S. hircicanis were reported (Hu et al., ). Through the above prevalence data, sarcocystosis seems to be a frequent infection even in developed countries (Savini, Dunsmore, Robertson, & Seneviratna, ), which requires the implementation of a worldwide preventive programme.…”

Section: Introductionmentioning

confidence: 99%

“…The prevalence of Sarcocystis spp. in goats is high, ranging between 77.3% and 99.4%, by microscopy and meat smears examination, respectively (Hu et al, 2016;Shekarforoush, Razavi, Dehghan, & Sarihi, 2005).…”

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confidence: 99%

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First detection and molecular identification of Sarcocystis spp. in small ruminants in North-West Tunisia

Amairia

Amdouni

Rouatbi

et al. 2017

Transbound Emerg Dis

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Sarcocystosis is a parasitic disease caused by varying Sarcocystis species infecting humans and animals. It is commonly found in small ruminants causing pathogenic effects. This contributes to detrimental economic loss for local farmers and the local economy due this disease. Although the distribution of Sarcocystis can be found all over the world, the species infecting small ruminants in Tunisia is still unknown. Through this study, we aim to estimate the molecular prevalence of natural infection with Sarcocystis spp. in sheep and goats using molecular identification. Also, phylogenetic analyses were used to identify the different species of this parasite infecting small ruminants in northern Tunisia for the first time. DNA was extracted from 198 and 121, sheep and goats meat samples, respectively. The molecular prevalence of Sarcocystis spp. in sheep and goats was 58.6% (116/198) and 50.4% (61/121), respectively. Compared to the Noire de Thibar and cross-breeds, the Barbarine sheep had the highest infection prevalence (63.4%) (p = .004). Five of the 116 positive samples were sequenced identifying Sarcocystis tenella from sheep. For goats, the sequencing results showed that five positive PCR products belonged to Sarcocystis capracanis species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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First detection and molecular identification of Sarcocystis spp. in small ruminants in North-West Tunisia

Amairia

Amdouni

Rouatbi

et al. 2017

Transbound Emerg Dis

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Morphological and molecular characteristics of six Sarcocystis spp. from red deer (Cervus elaphus) in Spain, including Sarcocystis cervicanis and three new species

et al. 2017

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Samples of muscle tissue from the diaphragm, oesophagus and/or heart of eight adult red deer (Cervus elaphus hispanicus) from the Quintos de Mora Park in Toledo, Central Spain, were screened for sarcocysts by means of the compression method. From positive samples, individual sarcocysts were excised and examined in wet mounts under a light microscope (LM) in order to study their basic morphology before being preserved for molecular studies. In all red deer examined, only microscopic sarcocysts were found. Those in the diaphragm and oesophagus were spindle-shaped and about 1 × 0.1 mm in size, while those in cardiac muscle were sac-like and 500-800 × 80-180 μm. By LM, the sarcocysts either had densely packed, about 8-μm-long, hair-like protrusions (type 1), sparsely distributed indistinct projections (fuzzy outline; type 2) or no visible protrusions (smooth surface; type 3). In cardiac muscle, only sarcocysts without visible protrusions were found. One of the latter sarcocysts was examined by scanning electron microscopy (SEM) and found to possess thin ribbon-like protrusions. Forty-eight sarcocysts isolated from the diaphragm, oesophagus and heart of one red deer, as well as 55 sarcocysts from the heart of three other red deer, 103 sarcocysts in total, were characterized molecularly through PCR amplification and sequencing of the partial cytochrome c oxidase subunit I gene (cox1) of the mitochondrial genome, revealing the presence of six major cox1 sequence types. Each type comprised either a single sequence (three types) or a collection of several identical or nearly identical sequences. From selected isolates possessing each of these cox1 sequence types, the complete 18S ribosomal RNA (rRNA) gene was amplified and sequenced directly and/or after cloning of the 5' end half. Supported by the sequence data from the latter gene, as well as the morphology of the sarcocysts from which the sequences originated, the six cox1 sequence types were considered to represent six separate Sarcocystis spp. Two cox1 sequence types were identified as belonging to the previously characterized species Sarcocystis hjorti (one sequence/sarcocyst) and Sarcocystis linearis (38 sequences/sarcocysts), respectively, whereas four sequence types were new. One of the latter types was assigned to the previously named species Sarcocystis cervicanis from red deer, since this sequence type was obtained from 52 sarcocysts from cardiac muscle, which matched the original morphological description (smooth surface) and habitat of this species. The remaining three sequence types were assigned to the three new species Sarcocystis iberica (one sequence/sarcocyst) Sarcocystis venatoria (10 sequences/sarcocysts) and Sarcocystis morae (one sequence/sarcocyst), respectively. The two species S. iberica and S. venatoria shared the same sarcocyst morphology (type 1) and habitat (diaphragm) and had virtually identical 18S rRNA gene sequences, but differed by 4% at cox1, which was considered sufficient to regard them as separate species. The single sarcocyst of S....

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Morphological and molecular characterizations of Sarcocystis miescheriana and Sarcocystis suihominis in domestic pigs (Sus scrofa) in China

Huang

Zhang

et al. 2019

Parasitol Res

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Prevalence, morphology, and molecular characteristics of Sarcocystis spp. in domestic goats (Capra hircus) from Kunming, China

Cited by 40 publications

References 28 publications

First detection and molecular identification of Sarcocystis spp. in small ruminants in North-West Tunisia

First detection and molecular identification of Sarcocystis spp. in small ruminants in North-West Tunisia

Morphological and molecular characteristics of six Sarcocystis spp. from red deer (Cervus elaphus) in Spain, including Sarcocystis cervicanis and three new species

Morphological and molecular characterizations of Sarcocystis miescheriana and Sarcocystis suihominis in domestic pigs (Sus scrofa) in China

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