Prevalence, incidence and risk factors for acquisition and colonization of extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae from dogs attended at a veterinary hospital in Spain

Ortiz-Díez, Gustavo; Mengíbar, Ruth Luque; Turrientes, María-Carmen; Artigao, María-Rosario Baquero; Gallifa, Raúl López; Tello, Alba Maroto; Pérez, Cristina Fernández; Santiago, Tania Ayllón

doi:10.1016/j.cimid.2022.101922

Cited by 8 publications

(4 citation statements)

References 84 publications

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“…Doxycycline was also included in this study because it is widely used in Cape Verde to treat a variety of bacterial infections in both veterinary and human medicine [ 62 , 63 , 64 ]. Resistance to this antimicrobial has already been reported among ESBL-positive Enterobacteriaceae species isolated from humans and dogs [ 65 , 66 ], which is in agreement with this study’s results.…”

Section: Discussionsupporting

confidence: 93%

ESBL-Positive Enterobacteriaceae from Dogs of Santiago and Boa Vista Islands, Cape Verde: A Public Health Concern

Matos

Cunha

Baptista³

et al. 2023

Antibiotics

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Antimicrobial resistance is a public health threat with an increasing expression in low- and middle-income countries such as Cape Verde. In this country, there is an overpopulation of dogs, which may facilitate the spread of resistant bacteria, including extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. To clarify the role of dogs as reservoirs for the dissemination of this bacterial group, 100 rectal swab samples were collected from confined (n = 50) and non-confined (n = 50) dogs in Santiago and Boa Vista Islands, Cape Verde. These were analyzed using conventional bacteriological techniques for the detection of ESBL-producing Enterobacteriaceae and characterization of their pathogenic and resistance profiles. Twenty-nine samples displayed ESBL-positive bacteria, from which 48 ESBL-producing isolates were obtained and mostly identified as Escherichia coli. Multiple antimicrobial resistance indexes ranged from 0.18 to 0.70 and half of the isolates were classified as multidrug-resistant. Isolates were capable of producing relevant virulence factors, including biofilm, showing virulence indexes between 0.29 and 0.71. As such, dogs in Cape Verde may act as reservoirs of resistant bacteria, including pathogenic and zoonotic species, representing a public health concern. Although further investigation is needed, this study proposes the periodical analysis of dogs’ fecal samples to monitor resistance dissemination in the country, in a One-Health perspective.

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Section: Discussionsupporting

confidence: 93%

ESBL-Positive Enterobacteriaceae from Dogs of Santiago and Boa Vista Islands, Cape Verde: A Public Health Concern

Matos

Cunha

Baptista³

et al. 2023

Antibiotics

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“…The unhealthy status of cats as a risk factor associated with the fecal carriage of ESBL/AmpC-producing E. coli observed in our study was likely influenced by the fact that a higher percentage of sick animals were treated with antibiotics compared to healthy cats. This suggests that antibiotic treatment alone may have increased the risk of the fecal carriage of AMR organisms in this study, as it is a well-recognized risk factor for the acquisition of drug-resistant bacteria in both humans and animals [ 2 , 46 , 47 ]. It was not possible to define if specific antibiotic classes were associated with ESBL/AmpC-producing E. coli due to the low number of cats treated with antibiotics in this study.…”

Section: Discussionmentioning

confidence: 99%

“…It was not possible to define if specific antibiotic classes were associated with ESBL/AmpC-producing E. coli due to the low number of cats treated with antibiotics in this study. The absence of an association between hospitalization and the fecal carriage of ESBL/AmpC-producing E. coli could have been due to the low number of hospitalized cats included in the present study or to the differences in management and hygiene protocols among hospitals [ 19 ], since hospitalization has been associated with the carriage of drug-resistant bacteria in companion animals [ 19 , 46 , 47 ]. In this respect, further studies focusing on hospitalized cats are needed to evaluate the contribution of veterinary hospitals in the spread of drug-resistant bacteria.…”

Section: Discussionmentioning

confidence: 99%

Fecal Carriage of Extended-Spectrum β-Lactamase-/AmpC-Producing Escherichia coli in Pet and Stray Cats

Ratti,

Facchin,

Stranieri

et al. 2023

Antibiotics

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Dogs have been reported as potential carriers of antimicrobial-resistant bacteria, but the role of cats has been poorly studied. The aim of this study was to investigate the presence and the risk factors associated with the fecal carriage of extended-spectrum β-lactamase and AmpC (ESBL/AmpC)-producing Escherichia coli (E. coli) in pet and stray cats. Fecal samples were collected between 2020 and 2022 from healthy and unhealthy cats and screened for ESBL/AmpC-producing E. coli using selective media. The presence of ESBL/AmpC-producing E. coli was confirmed by phenotypic and molecular methods. The evaluation of minimum inhibitory concentrations (MICs) was performed on positive isolates. Host and hospitalization data were analyzed to identify risk factors. A total of 97 cats’ samples were collected, and ESBL/AmpC-producing E. coli were detected in 6/97 (6.2%), supported by the detection of blaCTX-M (100%), blaTEM (83.3%), and blaSHV (16.7%) genes and the overexpression of chromosomal ampC (1%). All E. coli isolates were categorized as multidrug-resistant. Unhealthy status and previous antibiotic therapy were significantly associated with ESBL/AmpC-producing E. coli fecal carriage. Our results suggest that cats may be carriers of ESBL/AmpC-producing E. coli, highlighting the need for antimicrobial stewardship in veterinary medicine and an antimicrobial-resistance surveillance program focusing on companion animals, including stray cats.

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“…In this study, a total of 56 multi-resistant bacterial strains obtained as etiological agents from hospitalized dogs in a previous study performed at Alfonso X El Sabio University in Madrid were selected [ 2 ]. Of these, 30 strains, identified as S. pseudintermedius , showed the presence of the mec A gene, which confers resistance by producing a penicillin-binding protein PBP 2A, and 26 species of Enterobacteriaceae [ 43 ] (19 E. coli and 7 K. pneumoniae ) had at least one extended-spectrum beta-lactamase resistance gene ( bla CTX-M1 , bla CTX-M9 , bla SHV, bla TEM ). E. coli CECT 516 and S. pseudintermedius DSM 21284 were used as reference strains.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Activity of Allium cepa Organosulfur Derivatives against Canine Multidrug-Resistant Strains of Staphylococcus spp. and Enterobacteriaceae

Maroto-Tello,

Ayllón,

Aguinaga-Casañas

et al. 2024

Veterinary Sciences

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Background: The increase of multi-resistant bacteria, especially Staphylococcus spp. and Enterobacteriaceae, constitutes a challenge in veterinary medicine. The rapid growth of resistance is outpacing antibiotic discovery. Innovative strategies are needed, including the use of natural products like Allium species (Allium sativum L. and Allium cepa L.), which have been used empirically for centuries to treat infectious diseases in humans and farm and aquaculture animals due to their antibacterial properties. Methods: This study aimed to evaluate the in vitro activity of two Allium-derived compounds, propyl propane thiosulfinate (PTS) and propyl propane thiosulfonate (PTSO), against multi-resistant Staphylococcus spp. (n = 30) and Enterobacteriaceae (n = 26) isolated from dogs referred to a veterinary teaching hospital in Madrid. Results and Discussion: The results indicated the in vitro efficacy of PTSO/PTS against the tested bacterial strains, and 56.7% of Staphylococcus pseudintermedius and 53.8% of Enterobacteriaceae showed sensitivity to PTS and PTSO compared with classic antibiotics. In addition, 50% of S. pseudintermedius strains resistant to erythromycin, ibofloxacin, difloxacin and orbifloxacin and 50% of Enterobacteriaceae strains resistant to tetracycline and doxycycline were sensitive to PTS and PTSO. Although studies are needed to verify their efficacy in vivo, the combined use of PTS and PTSO exhibits promise in enhancing bacterial sensitivity against S. pseudintermedius and Enterobacteriaceae infections, providing a first insight into the potential of both compounds in veterinary practice.

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Prevalence, incidence and risk factors for acquisition and colonization of extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae from dogs attended at a veterinary hospital in Spain

Cited by 8 publications

References 84 publications

ESBL-Positive Enterobacteriaceae from Dogs of Santiago and Boa Vista Islands, Cape Verde: A Public Health Concern

ESBL-Positive Enterobacteriaceae from Dogs of Santiago and Boa Vista Islands, Cape Verde: A Public Health Concern

Fecal Carriage of Extended-Spectrum β-Lactamase-/AmpC-Producing Escherichia coli in Pet and Stray Cats

In Vitro Activity of Allium cepa Organosulfur Derivatives against Canine Multidrug-Resistant Strains of Staphylococcus spp. and Enterobacteriaceae

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