Prevalence Escherichia coli, Klebsiella and Enterobacter Species and AmpC-producing Enterobacteriaceae in Clinical Specimens of Hospitals Affiliated to Babol University of Medical Sciences, Iran using Phenotypic and Molecular Methods

Shahandeh, Zahra; Sadighian, Farahnaz; Kalantrai, Narges

doi:10.30699/ijmm.16.3.212

Cited by 6 publications

(4 citation statements)

References 27 publications

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“…26 AmpC production was detected in 13.1% isolates, which is slightly lower than the study done by Shivanna and Rao, 5 but similar to Mirza et al 8 Many studies too indicate boronic acid disc-diffusion test as a better method than other phenotypic methods to identify the producers of AmpC although no specific confirmatory phenotypic tests have been announced for the detection of AmpC enzymes by CLSI so far. 27 Our result for MBL production was found to be much higher (14.8%) than the studies done by Mirza et al, 8 but lower than that reported by Chanu et al 7 The difference in the prevalence rate in our study could also be endorsed due to various factors such as our hospital antibiotic guidelines and practices, ethnic differences in various populations, different phenotypic methods and procedures performed in other studies. 25 28…”

Section: Discussioncontrasting

confidence: 63%

Phenotypic Detection of ESBL, AmpC, MBL, and Their Co-occurrence among MDR Enterobacteriaceae Isolates

et al. 2022

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Background Emergence of extended-spectrum beta-lactamases (ESBLs), AmpC β-lactamases, and metallo-β lactamases (MBL), and their co-existence among members of Enterobacteriaceae pose newer diagnostic and therapeutic challenges. The present study examines the ESBL, AmpC, and MBL production by various phenotypic methods and their co-occurrence among the multidrug-resistant (MDR) Enterobacteriaceae clinical isolates. Materials and Methods Four hundred non-repetitive Enterobacteriaceae clinical isolates were collected from the Central Referral Hospital, Sikkim. The isolates were used for identification and their antibiotic susceptibility tests were performed according to the Clinical and Laboratory Standard Institute (CLSI) guidelines. ESBL was detected by double-disc synergy test (DDST) and phenotypic confirmatory disc-diffusion test (PCDDT), AmpC detection by AmpC E-test, and boronic acid disc diffusion (BD) test. MBL was detected using the imipenem–imipenem/EDTA disc and carba-NP tests. Results Around 76% were considered MDR. ESBL was seen in 58% and 50.4% based on DDST and phenotypic confirmation disc-diffusion test (PCDDT), respectively. AmpC was detected in 11.8% and 13.1% using a commercial E-test and boronic acid test, respectively. MBL were identified in 12.8% and 14.8% based on MBL imipenem-EDTA and carba-NP tests, respectively. Co-occurrence of ESBL and AmpC, ESBL and MBL, AmpC and MBL was seen in 5.2%, 11.5%, 1.3%, respectively, whereas a combination of these three β-lactamases was observed in only 0.3% of 304 MDR isolates. Conclusion The findings highlight a high prevalence of β-lactamases and their co-production among the Enterobacteriaceae, mainly in Klebsiella pneumoniae and Escherichia coli isolates. The study further highlights the necessity to identify the MDR β-lactamases stains for effective therapy in severe as well as mild bacterial infections, thereby enabling to reduce the risk of MDR in hospital and community settings.

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Section: Discussioncontrasting

confidence: 63%

Phenotypic Detection of ESBL, AmpC, MBL, and Their Co-occurrence among MDR Enterobacteriaceae Isolates

et al. 2022

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“…According to Shahandeh Z et al study in Iran out of 259 isolated E. coli, the ESBL-producing E. coli were 137 (52.9%). 15 In our study, out of 139 stool samples tested, 106 E. coli isolates were obtained; out of those isolates, 33.96% were carriage of resistance to one or more antibiotics and MDR was 7.55%. Resistance was most frequently observed to Ampicillin (27.36%) and a study in Tamil Nadu done by Seidman JC et al showed that ≥1 antibiotic resistance rate of 63% and multiple drug resistance of 32%, respectively, in 119 E. coli colony pools (stool samples) analyzed.…”

Section: Resultsmentioning

confidence: 71%

Fecal Carriage of Multi-drug Resistant Escherichia coli in Healthy Children: a Community-based Study

Sharma,

Gurung,

Devkota

et al. 2023

jhas

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Introduction: Escherichia coli is enteric Gram-negative bacilli, earlier recognized as non-invasive commensal. Invasive strains of E. coli have been identified and are associated with a variety of human infections. Increasing drug resistance among E. coli has been reported. Multidrug resistance is the ability of an organism to resist at least one agent in three or more antimicrobial categories. The aim of this study was to detect fecal carriage of MDR E. coli isolates among healthy school children. Methods: A total of 139 stool samples were collected from Rastriya Secondary School, Purano Tudikhel, Pokhara-1. Isolation, identification, and Antibiotic susceptibility test pattern of E. coli isolates were done by standard microbiological techniques. Further Extended Spectrum Beta-Lactamase test was performed by Combined Disc Test and data analysis was done by using Microsoft Excel. Results: A total of 106 E. coli were isolated from the stool samples. MDR E. coli were found to be 8 (7.55%) and 7 E. coli isolates were resistant to Ceftazidime which was further tested for ESBL and 5 isolates were found to be ESBL producers. The resistance of E. coli to Ampicillin, Nitrofurantoin, Ceftriaxone, Ceftazidime, Ciprofloxacin, and Gentamicin was 27.36%, 43%, 10.38%, 6.60%, 6.60%, and 2.83% respectively. Only one isolate was resistant to all antibiotics used except Imipenem. Conclusion: E. coli is the main Gram-negative bacteria isolated from the stool sample and identified as the main carrier of antimicrobial resistance in species comparison of Enterobacteriaceae isolated from fecal samples. Therefore, quick surveillance is needed in order to effectively handle such MDR strains. The E. coli population susceptible to all antibiotics used was a much more diverse group than the resistant and ESBL-producing E. coli.

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“…This study showed the prevalence of MBL in K. pneumoniae and E. coli to be 15.4%, and 9.4% respectively giving an overall prevalence of 12%. [27]. Bora et al [19] reported a prevalence of 18.98% and 21.08% in E coli and K. pneumoniae respectively in Nepal [19].…”

Section: Discussionmentioning

confidence: 97%

Metallo-Beta-Lactamase Producing Isolates of Escherichia coli and Klebsiella pneumoniae and their Resistance Profiles in Enugu, Nigeria: A Threat to Public Health

Maduakor,

Eleazar,

Mba

et al. 2024

JAMB

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Background: A potential threat to public health is the rapidly spreading enterobacteriaceae, especially Escherichia coli and Klebsiella pneumoniae which produce metallo-beta-lactamases (MBL). This study evaluated the prevalence of metallo-beta-lactamase (MBL) from clinical and non-clinical sources in Enugu Metropolis. Methodology: The study was conducted in the Microbiology Laboratory of the University of Nigeria Teaching Hospital, Ituku-Ozalla between October 2020 and July 2021. A total of 150 isolates including 85 and 65 isolates of Escherichia coli and Klebsiella pneumoniae respectively was recovered. Standard microbiology procedures were used to identify and characterize the isolates. Antimicrobial susceptibility was done using the Kirby-Bauer disc diffusion technique. Phenotypic detection of Metallo-beta-lactamase production was determined using Combined Disk Tests. Results: Imipenem resistance was detected in 22 (25.9%) isolates of E. coli and 18 (27.7%) isolates of K. pneumoniae. Of the 22 strains of E. coli that were imipenem resistant, 8 (9.4%) and 14 (16.5%) were found to be MBL producers and non-MBL respectively. Of the 18 strains of Klebsiella pneumoniae that were imipenem resistant, 10 (15.4%) were MBL producers and 8 (12.3%) were non-MBL producers. The highest prevalence of MBL was recovered from urine sources in both E. coli and K. pneumonieae. All MBL-producing isolates were multidrug resistant. Conclusion: The overall prevalence of MBL in this study was 12.0%. Public health is at risk due to the occurrence of metallo-beta-lactamase. Antimicrobial stewardship and the implementation of infection control strategies are required to halt the spread of these resistant bacteria in the environment. The use of antibiotics should be with utmost prudence.

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Prevalence Escherichia coli, Klebsiella and Enterobacter Species and AmpC-producing Enterobacteriaceae in Clinical Specimens of Hospitals Affiliated to Babol University of Medical Sciences, Iran using Phenotypic and Molecular Methods

Cited by 6 publications

References 27 publications

Phenotypic Detection of ESBL, AmpC, MBL, and Their Co-occurrence among MDR Enterobacteriaceae Isolates

Phenotypic Detection of ESBL, AmpC, MBL, and Their Co-occurrence among MDR Enterobacteriaceae Isolates

Fecal Carriage of Multi-drug Resistant Escherichia coli in Healthy Children: a Community-based Study

Metallo-Beta-Lactamase Producing Isolates of Escherichia coli and Klebsiella pneumoniae and their Resistance Profiles in Enugu, Nigeria: A Threat to Public Health

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