Prevalence, antimicrobial susceptibility and plasmid profiling of Vibrio spp. isolated from cultured groupers in Peninsular Malaysia

Amalina, Nor Zulkiply; Silvaraj, Santha; Zulperi, Dzarifah; Amal, MNA; Yusof, Mohd Termizi; Zamri‐Saad, Mohd; Yasin, Ina Salwany Md

doi:10.21203/rs.2.16094/v2

Cited by 3 publications

(2 citation statements)

References 39 publications

(53 reference statements)

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“…Other studies from Iran, South‐East Asian countries and China reported prevalence values between 0% and 58.6%, with a slightly lower occurrence in fish than in crustacean (e.g. shrimps and prawns) (Elhadi et al., ; Yano et al., ; Gopal et al., ; Ji et al., ; Koralage et al., ; Raissy et al., ; Pan et al., ; Paydar and Thong, ; Tey et al., ; Wong et al., ; Tra et al., ; Amalina et al., ; Yan et al., ), while high detection rates were reported for fish from Mexico Gulf (37–51%) and for crabs from the Atlantic coast of USA (Tao et al., ; Baumeister et al., ; Rodgers et al., ). As for V. parahaemolyticus , according to a recent meta‐analysis of available studies, the prevalence of this species in seafood amounts to 48.3% (95% CI: 0.454–0.512) in crustaceans and to 51.0% (95% CI: 0.476–0.544) in fish and cephalopod (Odeyemi, ).…”

Section: Appendix a – Search Strategies And Outcome Of The Literature Searches Supporting The Hazard Identificationmentioning

confidence: 96%

The use of the so‐called ‘tubs’ for transporting and storing fresh fishery products

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2020

EFS2

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On‐land transport/storage of fresh fishery products (FFP) for up to 3 days in ‘tubs’ of three‐layered poly‐ethylene filled with freshwater and ice was compared to the currently authorised practice (fish boxes of high‐density poly‐ethylene filled with ice). The impact on the survival and growth of biological hazards in fish and the histamine production in fish species associated with a high amount of histidine was assessed. In different modelling scenarios, the FFP are stored on‐board in freshwater or seawater/ice (in tubs) and once on‐land they are ‘handled’ (i.e. sorted or gutted and/or filleted) and transferred to either tubs or boxes. The temperature of the FFP was assumed to be the most influential factor affecting relevant hazards. Under reasonably foreseeable ‘abusive’ scenarios and using a conservative modelling approach, the growth of the relevant hazards (i.e. Listeria monocytogenes, Aeromonas spp. and non‐proteolytic Clostridium botulinum), is expected to be < 0.2 log10 units higher in tubs than in boxes after 3 days when the initial temperature of the fish is 0°C (‘keeping’ process). Starting at 7°C (‘cooling‐keeping’ process), the expected difference in the growth potential is higher (< 1 log10 for A. hydrophila and < 0.5 log10 for the other two hazards) due to the poorer cooling capacity of water and ice (tub) compared with ice (box). The survival of relevant hazards is not or is negligibly impacted. Histamine formation due to growth of Morganella psychrotolerans under the ‘keeping’ or ‘cooling‐keeping’ process can be up to 0.4 ppm and 1.5 ppm higher, respectively, in tubs as compared to boxes after 3 days, without reaching the legal limit of 100 ppm. The water uptake associated with the storage of the FFP in tubs (which may be up to 6%) does not make a relevant contribution to the differences in microbial growth potential compared to boxes.

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Section: Appendix a – Search Strategies And Outcome Of The Literature Searches Supporting The Hazard Identificationmentioning

confidence: 96%

The use of the so‐called ‘tubs’ for transporting and storing fresh fishery products

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2020

EFS2

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“…as it resistant to at least two antibiotics. MAR index with value more than 0.2 indicate a 'highrisk' source of antibiotic contamination [20]. The location was in the urban area that affected by the residential area, industrial activities and aquaculture farm.…”

Section: Mar Index Analysismentioning

confidence: 99%

Isolation of Antibiotic Resistant Bacteria from Rivers in Terengganu, Malaysia

Hassan

Yern

Zain

2020

International Journal of Life Sciences and Biotechnology

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Infectious disease cause by antibiotic resistant bacteria has been more difficult to treat because the ability of these bacteria to avoid the effect of antibiotic medication. Nowadays, water environment especially river has become the reservoir for the dissemination of antibiotic resistant bacteria due to antibiotic contamination derived from human activities. The exposure to antibiotic resistance bacteria in water increase the health risks associated with the waterborne bacteria in animals and humans. Therefore, this study was carried out to investigate the presence of antibiotic resistant bacteria from two rivers; Ibai River and Terengganu River in Terengganu. A total of 24 bacterial strains were isolated from both rivers. Antibiotic susceptibility of the bacteria isolates was assayed according to the Kirby-Bauer disk diffusion method using six types of antibiotics such as ampicillin, rifampicin, gentamicin, tetracycline, ciprofloxacin and chloramphenicol. The results of this study indicate that 100% of bacterial strains were resistant to at least two antibiotics tested. The multiple antibiotic resistant (MAR) index of these bacteria was calculated and 23 bacterial strains show MAR index of more than 20%. Among the 23 isolates, a total of 10 bacterial strains (2A, 2B, 2C, 2I, 3A, 3B, 4A, 4C, 4D and 4E) were successfully identified as Chromobacterium violaceum, Enterobacter tabaci, Bacillus wiedmannii, Acinetobacter baumannii, Klebsiella quasipneumoniae subsp. quasipneumoniae, Chromobacterium aquaticum, Cupriavidus metallidurans, Weeksella massiliensis, Cupriavidus necator and Acinetobacter nosocomialis. The results from this study show that both of the rivers are exposed to high level of antibiotics compound which may lead the bacteria to develop mechanism of antibiotic resistant. This may cause a serious threat to the public health and environment.

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Diversity, Relative Abundance, and Functional Genes of Intestinal Microbiota of Tiger Grouper (Epinephelus fuscoguttatus) and Asian Seabass (Lates calcarifer) Reared in A Semi-Closed Hatchery in Dry and Wet Seasons

Sutra¹,

Saadu²,

Hashim³

et al. 2021

JTAS

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Grouper and Asian seabass are among the economically important cultured marine fish in Malaysia. However, fry productions in large scale tend to introduce stress that changes the fish microbiota and increases susceptibility to diseases. Currently, high-throughput sequencing is used to study fish microbiota and their respective gene functions. In this study, we investigate the diversity, abundance and functional genes of intestinal microbiota of tiger grouper and Asian seabass that were reared in a semi-closed hatchery during dry and wet seasons. Intestinal samples were collected from tiger grouper and Asian seabass of different sizes before proceeded to DNA extraction. The extracted DNA were then subjected to 16S rRNA gene amplicon sequencing using the Illumina Miseq platform targeting V3 and V4 regions for determination of the bacterial diversity, abundance and functional genes in both seasons. The results revealed that intestinal microbiota of Asian seabass were dominated by the phylum Proteobacteria and order Vibrionales in both seasons. Meanwhile, intestinal microbiome of tiger groupers were shifted from domination of phylum Firmicutes and order Clostridiales in dry season to Proteobacteria and order Lactobacillales in wet season. PICRUSt analysis revealed that the functional genes that were dominantly present were the genes encoded for metabolism, genetic information processing, environmental information processing, cellular process and human diseases. Remarkably, SIMPER analysis showed several potential metagenomics biomarker genes in dry and wet seasons. This study revealed the importance of utilizing amplicon metagenomics approaches in microbiome studies for better identification of the microbial profiling in aquaculture systems.

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Prevalence, antimicrobial susceptibility and plasmid profiling of Vibrio spp. isolated from cultured groupers in Peninsular Malaysia

Cited by 3 publications

References 39 publications

The use of the so‐called ‘tubs’ for transporting and storing fresh fishery products

The use of the so‐called ‘tubs’ for transporting and storing fresh fishery products

Isolation of Antibiotic Resistant Bacteria from Rivers in Terengganu, Malaysia

Diversity, Relative Abundance, and Functional Genes of Intestinal Microbiota of Tiger Grouper (Epinephelus fuscoguttatus) and Asian Seabass (Lates calcarifer) Reared in A Semi-Closed Hatchery in Dry and Wet Seasons

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