Prevalence, antimicrobial susceptibility and plasmid profiling of Vibrio spp. isolated from cultured groupers in Peninsular Malaysia

Abstract

BackgroundNumerous prevalence studies of Vibrio spp. infection in fish have been extensively reported worldwide, including Malaysia. Unfortunately, information on the prevalence of Vibrio spp. in groupers (Epinephelus spp.) is limited. In this study, groupers obtained from nine farms located at different geographical regions in Malaysia were sampled for the presence of pathogenic Vibrio spp. and their susceptibility profiles against seven antibiotics.ResultsOut of 270 grouper samples, 195 (72%) were detected with the presence of Vibrio spp. Vibrio communis showed highest prevalence in grouper (28%), followed by V. parahaemolyticus (25%), V. alginolyticus (19%), V. vulnificus (14%), V. rotiferianus (3%), Vibrio sp. (3%), V. campbellii (2%), V. mytili (2%), V. furnissii (2%), V. harveyi (1%), V. tubiashii (1%), V. fluvialis (0.3%) and V. diabolicus (0.3%). Assessment on the antibiotic susceptibility profiles of the Vibrio spp. revealed that majority of the isolates were susceptible to tetracycline, streptomycin, erythromycin and bacitracin, but resistance to ampicillin, penicillin G and vancomycin. The mean MAR index of the Vibrio isolates was 0.51, with 85% of the isolates showed MAR index value of higher than 0.2. Results indicate that the Vibrio spp. were continuously exposed to antibiotics. Furthermore, the plasmid profiles of Vibrio spp. showed that 38.7% of the isolates harbored plasmid with molecular weight of more than 10 kb, while 61.3% were without plasmid. During curing process, Vibrio spp. lost their plasmid, but remained resistant to ampicillin, penicillin G, bacitracin and vancomycin while a few isolates remained resistant to erythromycin, streptomycin and tetracycline. The results suggested that the resistance to antibiotics in isolated Vibrio spp. might be due to chromosomal and plasmid borne.ConclusionsThis study demonstrates the prevalence of Vibrio spp. in groupers and the distribution of multidrug resistance strains that could be of concern to the farmers in Malaysia. In addition, data from this study can be further used in fish disease management plan.

Vibrio alginolyticus in marine foods of animal origin from establishments in the port of Chabihau, Yucatan, Mexico

“…The results obtained in the present work are consistent with the results obtained in studies carried out by other authors (Elhadi et al, 2004;Jacobs et al, 2017;Vu et al, 2018;Amalina et al, 2019;Sadat et al, 2020).…”

“…This study demonstrates the prevalence of Vibrio spp., in groupers and the distribution of multidrug resistance strains that could be of concern to the farmers in Malaysia. In addition, data from this study can be further used in fish disease management plan (Amalina et al, 2019).…”

Antibiotic resistant Vibrio harveyi isolated from shrimp pond and anti-Vibrio activity of Combretum quadrangulare Kurz. and Mimosa pudica extracts

“…The findings were also in agreement with studies of Parvathi et al (2011) which presented various antibiotic especially penicillin and bacitracin. In contrast to previous studies, it is reported that Vibrio isolates (56%) were susceptible against bacitracin (Parvathi et al, 2011;Amalina et al, 2019).…”

Diversity, Relative Abundance, and Functional Genes of Intestinal Microbiota of Tiger Grouper (Epinephelus fuscoguttatus) and Asian Seabass (Lates calcarifer) Reared in A Semi-Closed Hatchery in Dry and Wet Seasons

“…Huang et al, 2018). Methyl-accepting chemotaxis protein (MCP) (K03406) was present in Asian seabass, and was associated with the genus Vibrio, which is known for its pathogenicity and disease outbreaks (Amalina et al, 2019;Mohamad et al, 2019aMohamad et al, , 2019b. MCP is a sensory transducer that controls exopolysaccharides (EPS) production (Xu et al, 2011).…”